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Enhancement of Energy Conservation by Hill Reaction Inhibitors in Isolated Spinach (Spinacia oleracea) Chloroplast Fragments

Published online by Cambridge University Press:  12 June 2017

G. J. Bethlenfalvay  and
P. A. Castelfranco
G. J. Bethlenfalvay
Affiliation:
Univ. of California, Davis, CA 95616
P. A. Castelfranco
Affiliation:
Univ. of California, Davis, CA 95616
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Abstract

The effect of diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], desmedipham [ethyl m-hydroxycarbanilate carbanilate(ester)], propanil (3′,4′-dichloropropionanilide), and dibromothymoquinone (DBMIB) (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) on proton translocation and photophosphorylation in isolated spinach (Spinacia oleracea L.) chloroplast fragments was investigated. In the absence of added cofactors, O2, or artificial electron acceptors, cyclic electron transport occurred, which was coupled to energy conservation. Under aerobic conditions O2 acted as the terminal acceptor in non-cyclic electron transport. Proton translocation and photophosphorylation in the cyclic process were enhanced by diuron, desmedipham, and propanil, while in the non-cyclic process they were inhibited by all three herbicides. DBMIB inhibited proton translocation and photophosphorylation in both processes. Proton translocation and its enhancement increased with increasing light intensities. The finding that the plastoquinone (PQ) antagonist DBMIB disrupted cyclic as well as noncyclic electron flow, while diuron enhanced the cyclic and inhibited the noncyclic process, indicated that the acceptor site for endogenously-cycling electrons must lie between the active site of diuron inhibition and PQ, The close similarity in the behavior of diuron, desmedipham, and propanil suggests that their site of action is the same.

Type
Research Article
Information
Weed Science , Volume 26 , Issue 1 , January 1978 , pp. 84 - 89
Copyright
Copyright © 1978 by the Weed Science Society of America 

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References

Literature Cited

1. Avron, M. and Chance, B. 1966. Relation of photophosphorylation to electron transport in isolated chloroplasts. Brookhaven Symp. Biol. 19:149–160.Google Scholar
2. Avron, M. and Neuman, J. 1968. Photophosphorylation in chloroplasts. Annu. Rev. Plant Physiol. 19:137–160.CrossRefGoogle Scholar
3. Avron, M. 1975. The electron transport chain in chloroplasts. Pages 373–386 in Govindjee, ed. Bioenergetics of Photosynthesis. Academic Press, New York.Google Scholar
4. Böhme, H. and Cramer, W. A. 1972. Localization of a site of energy coupling between plastoquinone and cytochrome f in the electron transport chain of spinach chloroplasts. Biochemistry 11:1155–1160.Google Scholar
5. Böhme, H., Reiner, S., and Trebst, A. 1971. The effect of dibromothymoquinone, an antagonist of plastoquinone on non-cyclic and cyclic electron flow systems in isolated chloroplasts. Z. Naturforsch. 26b:341–352.Google Scholar
6. Forti, G. and Zanetti, J. 1969. The electron pathway of cyclic photophosphorylation. Pages 1213–1216 in Metzner, H., ed. Progress in Photosynthesis Research. Vol. III. Tubingen.Google Scholar
7. Gould, J. M., Izawa, S., and Good, N. E. 1973. A site of photophosphorylation close to photosystem II. Proc. Fed. Am. Soc. Exp. Biol. 43:632.Google Scholar
8. Gould, J. M. 1975. The phosphorylation site associated with the oxidation of exogenous donors of electrons to photosystem I. Biochim. Biophys. Acta 387:135–148.Google Scholar
9. Gould, J. M. and Izawa, S. 1973. Photosystem II electron transport and phosphorylation with dibromothymoquinone as the electron acceptor. Eur. J. Biochem. 37:185–192.Google Scholar
10. Hansch, C. 1969. Theoretical considerations of the structure-activity relationship in photosynthetic inhibitors. Pages 1685–1692 in Metzner, H., ed. Progress in Photosynthesis Research. Vol. III. Tubingen.Google Scholar
11. Hauska, G., Oettmeier, W., Reimer, S., and Trebst, A. 1975. Shuttles of artificial electron donors for Photosystem I across the thylakoid membrane. Z. Naturforsch. 30c: 37–45.CrossRefGoogle Scholar
12. Hauska, G., Reimer, S., and Trebst, A. 1974. Native and artificial energy conserving sites in cyclic photophosphorylation systems. Biochim. Biophys. Acta 357:1–13.Google Scholar
13. Hauska, G. and Prince, R. 1974. Artificial proton translocation by lipophilic, quinoid hydrogen carriers in chloroplasts and liposomes. FEBS Lett. 41:35–39.Google Scholar
14. Heber, U. 1973. Electronentransport zum Sauerstoff und ATP Verbrauch in der Photosynthese. Ber. Dtsch. Bot. Ges. 86:187–195.Google Scholar
15. Heber, U. 1969. Conformational changes in chloroplasts induced by illumination of leaves in vivo. Biochim. Biophys. Acta 180:302–319.Google Scholar
16. Heber, U. and French, C. S. 1968. Effects of oxygen on the electron transport chain of photosynthesis. Planta 79:99–112.Google Scholar
17. Hind, G. and Jagendorf, A. T. 1963. Separation of light and dark states in photophosphorylation. Proc. Nat. Acad. Sci. U.S.A. 49:715–722.Google Scholar
18. Izawa, S., Gould, J. M., Ort, D. R., Felker, P., and Good, N. E. 1973. Electrontransport and photophosphorylation in chloroplasts as a function of the electron acceptor. Biochim. Biophys. Acta 305:119–128.CrossRefGoogle Scholar
19. Jagendorf, A. T. and Hind, G. 1963. Studies on the mechanism of photophosphorylation. Pages 699–710 in Kok, B., ed. Photosynthetic Mechanisms of Green Plants. Publication 1145, Natl. Acad. Sci., Natl. Res. Council, Washington, D.C. Google Scholar
20. Junge, W. and Ausländer, W. 1974. The electric generator in photosynthesis in green plants. 1. Vectorial and protolytic properties of the electron transport chain. Biochim. Biophys. Acta 333:59–70.Google Scholar
21. Kaiser, W. and Urbach, W. 1973. Endogene zyklische Photophosphorylierung in isolierten Chloroplasten. Ber. Dtsch. Bot. Ges. 86:213–226.Google Scholar
22. Krause, G. H. 1973. Einfluss des Sauerstoffs auf den Energiezustand der belichteten Blattzelle. Ber. Dtsch. Bot. Ges. 86:197–202.Google Scholar
23. Lamprecht, W. and Trautschold, I. 1963. ATP determination with hexokinase and glucose-6-phosphate dehydrogenase. Pages 543–551 in Bergmeyer, H. V., ed. Methods in Enzymatic Analysis.Google Scholar
24. Mehler, A. H. 1951. Studies of the reactions of illuminated chloroplasts. 1. Mechanism of reduction of oxygen and other Hill reagents. Arch. Biochem. Biophys. 33:65–77.Google Scholar
25. Migniac-Maslow, M. 1971. Etude de la photophosphorylation endogene des chloroplastes isole d epinard. Biochim. Biophys. Acta 234:353–359.Google Scholar
26. Mitchell, P. 1966. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol. Rev. Cambridge Philos. Soc. 41:445–502.Google Scholar
27. Ort, D. R. 1975. Quantitative relationship between photosystem I electron transport and ATP formation. Arch. Biochem. Biophys. 166:629–638.Google Scholar
28. Reinwald, E., Stiehl, H. H., and Rumberg, B. 1968. Correlation between plastoquinone reduction, field formation, and proton translocation in photosynthesis. Z. Naturforsch. 23b:1616–1625.Google Scholar
29. Rensen Van, J. J. S. 1969. Polyphosphate formation in Scenedesmus in relation to photosynthesis. Pages 1769–1776 in Metzner, H., ed. Progress in Photosynthesis Research. Vol. III. Tubingen.Google Scholar
30. Schürmann, B., Buchanan, B., and Arnon, D. I. 1972. The role of cyclic photophosphorylation in photosynthetic carbon dioxide assimilation by isolated chloroplasts. Biochim. Biophys. Acta 267:111–124.Google Scholar
31. Trebst, A. 1974. Energy conservation in photosynthetic electron transport in chloroplasts. Annu. Rev. Plant Physiol. 25:423–458.CrossRefGoogle Scholar
32. Trebst, A. and Reimer, S. 1973. Properties of photoreductions by photosystem II in isolated chloroplasts. An energy conserving step in the photoreduction of benzoquinones by photosystem II in the presence of dibromothymoquinone. Biochim. Biophys. Acta 305:129–139.CrossRefGoogle ScholarPubMed
33. Walker, D. A. 1971. Cellular and subcellular preparations; Chloroplasts: aqueous. Pages 211–220 in San Pietro, A., ed. Methods of Enzymology. Vol. 23.Google Scholar
34. Witt, H. T. 1974. Primary acts of energy conservation in the functional membrane of photosynthesis. Annals N.Y. Acad. Sci. 227:203–206.Google Scholar