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Effects of 2,4-D and Other Herbicides on Oxidative Phosphorylation in Mitochondria from Cabbage

Published online by Cambridge University Press:  12 June 2017

Prabhakar D. Lotlikar ,
LeMar F. Remmert  and
Virgil H. Freed
Prabhakar D. Lotlikar
Affiliation:
Department of Agricultural Chemistry, Oregon Agricultural Experiment Station and Department of Chemistry, Oregon State University, Corvallis
LeMar F. Remmert
Affiliation:
Department of Agricultural Chemistry, Oregon Agricultural Experiment Station and Department of Chemistry, Oregon State University, Corvallis
Virgil H. Freed
Affiliation:
Department of Agricultural Chemistry, Oregon Agricultural Experiment Station and Department of Chemistry, Oregon State University, Corvallis
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Abstract

The effects of 22 herbicides on oxidative phosphorylation in mitochondria from cabbage (Brassica oleracea var. capitata L.) have been studied. Carbamates, phenoxyacids, 3,5-dimethyltetrahydro-l,3,5,2H-thiadiazine-2-thione (DMTT), N-1-napthylphthalamic acid (NPA), 2,3,6-trichlorobenzoic acid (2,3,6-TBA), 3-(p-chlorophenyl)-1,1-dimethylurea (monuron), and 2-chloro-2-N,N-diallylacetamide (CDAA) inhibited phosphorylation to a greater extent than oxygen uptake in cabbage mitochondria. The compounds 1,2-dihydropyridazine-3,6-dione (MH), 2,2-dichloropropionic acid (dalapon), 2,2,3-trichloropropionic acid (hereinafter referred to as 2,2,3-TPA), sodium chlorate, and 3-amino-l,2,4-triazole (amitrole) at concentrations as high as 1 × 10–2 M did not have a large effect on oxidative phosphorylation in cabbage mitochondria. Respiration which was stimulated by 2,4-dinitrophenol (DNP) or adenosine diphosphate (ADP) was inhibited by 2,4-dichlorophenoxyacetic acid (2,4-D). Although 2,4-D did not affect Mg++-stimulated adenosine triphosphatase (ATPase), it inhibited an oleate-stimulated ATPase activity and the ATP-32Pi exchange reaction in cabbage mitochondria. The results suggest that 2,4-D may inhibit respiration in cabbage mitochondria by an effect on a reaction involved in coupling phosphorylation with electron transport.

Type
Research Article
Information
Weed Science , Volume 16 , Issue 2 , April 1968 , pp. 161 - 165
Copyright
Copyright © Weed Science Society of America 

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References

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