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Effect of Picloram on Organogenesis in Detached Leaves of Echeveria elegans Bgr.

Published online by Cambridge University Press:  12 June 2017

M. V. S. Raju  and
R. Grover
M. V. S. Raju
Affiliation:
Dep. Biol., Univ. Regina
R. Grover
Affiliation:
Dep. Biol., Univ. Regina
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Abstract

The effect of picloram (4-amino-3,5,6-trichloropicolinic acid) on regeneration of detached leaves of Echeveria elegans Bgr. was studied. Picloram stimulated callus production at all concentrations. Low concentrations of 1 to 15 ppmw picloram did not affect shoot organogenesis and the same, however, stimulated rhizogenesis. Inhibition of both root- and shoot-organogenesis was noticeable at concentrations below 20 ppmw and above. Roots increased in number at all picloram concentrations below 40 ppmw, the maximum number being at concentration of 15 to 20 ppmw of the herbicide. Observations indicated that root elongation was considerably suppressed. Picloram-induced callus contained mature parenchyma cells devoid of contents and considerable amount of tissue disruption was noticed. Irregular vascular differentiation and obliteration of tissues particularly in the region of tracheary elements, were obvious. Although normal apical organization was lacking in root primordia and in arrested short roots, these elongated normally into mature roots when they were transferred to water, thus indicating that apical meristems were not severely affected by picloram.

Type
Research Article
Information
Weed Science , Volume 24 , Issue 2 , March 1976 , pp. 175 - 182
Copyright
Copyright © 1976 by the Weed Science Society of America 

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References

Literature Cited

1. Chang, G. and Foy, C.L. 1971. Effect of picloram on germination and seedling development of four species. Weed Sci. 19:58–64.CrossRefGoogle Scholar
2. Chen, L.G., Switzer, C.M., and Fletcher, R.A. 1972. Nucleic acid and protein changes induced by auxin-like herbicides. Weed Sci. 20:53–55.CrossRefGoogle Scholar
3. Crafts, A.S. and Crisp, C.E. 1971. Phloem transport in plants. W.H. Freeman and Co., San Francisco. 481 pp.Google Scholar
4. Davis, E. and Maclachlan, G.A. 1969. Generation of cellulase activity during protein synthesis by pea microsomes in vitro . Archiv. Biochem. Biophys. 129:581–587.CrossRefGoogle Scholar
5. Diaz-Colon, J.D., Bovey, R.W., Davis, F.S., and Baur, J.R. 1972. Comparative effects and concentration of picloram, 2,4,5-T and dicamba in tissue culture. Physiol. Plant. 27:60–64.CrossRefGoogle Scholar
6. Eisinger, W.R. and Morre, D.J. 1971. Growth-regulator properties of picloram, 4-amino-3,5,6-trichloropicolinic acid. Can. J. Bot. 49:889–897.CrossRefGoogle Scholar
7. Eisinger, W.R., Morre, D.J., and Hess, C.E. 1966. Promotion of plant growth by Tordon herbicide. Down Earth 21(4):8–10.Google Scholar
8. Greenwood, M.S. and Hodgson, D.H. 1970. The effect of picloram (4-amino-3,5,6-trichloropicolinic acid) on root regeneration: a comparison with IAA and other auxins. Amer. J. Bot. (Abs.) 57:762.Google Scholar
9. Hamaker, J.W., Johnston, J.H., Martin, T.R., and Redemann, C.T. 1963. A picolinic acid derivative; a plant growth regulator. Science 141:363.CrossRefGoogle ScholarPubMed
10. Johansen, D.A. 1940. Plant Microtechnique. McGraw-Hill, New York. 523 pp.Google Scholar
11. Kefford, N.P. and Caso, H.O. 1966. A potent auxin with unique chemical structure 4-amino-3,5,6-trichloropicolinic acid. Bot. Gaz. 127:159–163.CrossRefGoogle Scholar
12. Klingman, G.C. and Guedez, H. 1967. Picloram and its effect on field-grown tobacco. Weeds 15:142–146.CrossRefGoogle Scholar
13. Kreps, L.B. and Alley, H.P. 1967. Histological abnormalities induced by picloram on Canada thistle roots. Weeds 15:56–59.CrossRefGoogle Scholar
14. Lee, G.A., Dobrenz, A.K., and Alley, H.P. 1967. Preliminary investigations of the effect of Tordon and 2,4-D on leaf and root tissue of Canada thistle. Down Earth 23(2):21–23.Google Scholar
15. Malhotra, S.S. and Hanson, J.B. 1970. Picloram sensitivity and nucleic acids in plants. Weed Sci. 18:1–4.CrossRefGoogle Scholar
16. National Research Council of Canada. 1974. Picloram: The Effects of its Use as a Herbicide on Environmental Quality. Nat. Res. Council of Canada. No. 13684, 128 pp.Google Scholar
17. Raju, M.V.S. and Hines, J.E. 1973. Regenerative studies on the detached leaves of Echeveria elegans. Regeneration and growth in leaves. Can. J. Bot. 51:451–456.CrossRefGoogle Scholar
18. Raju, M.V.S. and Mann, H.E. 1970. Regenerative studies on the detached leaves of Echeveria elegans. Anatomy and regeneration of leaves in sterile culture. Can. J. Bot. 48:1887–1891.CrossRefGoogle Scholar
19. Raju, M.V.S. and Mann, H.E. 1971. Regenerative studies on the detached leaves of Echeveria elegans. Patterns of regeneration of leaves in sterile culture. Can. J. Bot. 49:2015–2021.CrossRefGoogle Scholar
20. Wu, C.C., Kozolowski, T.T., Evert, R.F., and Sasaki, S. 1971. Effects of direct contact of Pinus resinosa seeds and young seedlings with 2,4-D or picloram on seedling development. Can. J. Bot. 49:1737–1741.CrossRefGoogle Scholar