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Effect of Paraquat on Dark-Grown Phaseolus vulgaris Cells

Published online by Cambridge University Press:  12 June 2017

Hagit Zer ,
Mordechai Chevion  and
Israel Goldberg
Hagit Zer
Affiliation:
Dep. Cellular Biochem., Hebrew Univ.-Hadassah Med. School, Jerusalem 91010 Israel
Mordechai Chevion
Affiliation:
Dep. Cellular Biochem., Hebrew Univ.-Hadassah Med. School, Jerusalem 91010 Israel
Israel Goldberg
Affiliation:
Dep. Applied Microbiol., Hebrew Univ.-Hadassah Med. School, Jerusalem 91010 Israel
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Abstract

Paraquat is known to affect all green plants and other eukaryotic organisms including mammalian cells. The aim of this study was to improve the understanding of paraquat toxicity in nonphotosynthetic plant cells using dark-grown kidney bean cells in tissue culture. It is shown that uptake of paraquat is an active process, and that paraquat inhibits cell growth, reduces DNA synthesis, and inhibits the activity of hydroxypyruvate reductase while enhancing the activity of glutathione reductase which is involved in cellular defense against oxidant stress. Additionally, it is demonstrated that iron ions are involved in paraquat toxicity. We conclude that uptake of paraquat into cells is via polyamine channels and that the deleterious effects of paraquat on these nonphotosynthetic cells are mediated by iron.

Keywords

Paraquat, 1,1’ dimethyl-4,4’ bipyridinium ion, kidney bean(Phaseolus vulgaris L. ‘BRTIL WAX’)Free radicalssperminepolyaminesiron
Type
Physiology, Chemistry and Biochemistry
Information
Weed Science , Volume 41 , Issue 4 , December 1993 , pp. 528 - 533
Copyright
Copyright © 1994 by the Weed Science Society of America 

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