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Effect of Herbicides on In Vivo Nitrate and Nitrite Reduction

Published online by Cambridge University Press:  12 June 2017

R.L. Finke
Affiliation:
Dep. Of Agron. And Soils, Washington State Univ., Pullman, WA 99163
R.L. Warner
Affiliation:
Dep. Of Agron. And Soils, Washington State Univ., Pullman, WA 99163
T.J. Muzik
Affiliation:
Dep. Of Agron. And Soils, Washington State Univ., Pullman, WA 99163

Abstract

The effects of herbicides on in vivo nitrate and nitrite reduction were determined by vacuum infiltrating sections of barley (Hordeum vulgare L.) or bean (Phaseolus vulgaris L.) leaves with solutions containing nitrate and herbicides. Herbicides causing a reduction of nitrite accumulation in the dark were considered to have inhibitory effects upon nitrate reduction and those causing an accumulation of nitrite in the light were considered to inhibit nitrite reduction. Only dinoseb (2-sec-butyl-4,6-dinitrophenol) and potassium azide significantly reduced nitrate reduction in both barley and bean. All of the herbicides which inhibit photosynthesis inhibited nitrite reduction but had no significant effect on nitrate reduction in barley and bean. Nitrite reduction in an atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] resistant pigweed (Amaranthus retroflexus L.) biotype was not affected by any triazine tested. However, these triazines significantly inhibited nitrite reduction in barley, bean, and the susceptible pigweed biotype. The results suggest that the in vivo nitrate reductase technique may be a useful technique for identifying chemicals which inhibit the flow of electrons to ferredoxin, thereby inhibiting nitrite reduction in light.

Type
Research Article
Copyright
Copyright © 1977 by the Weed Science Society of America 

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