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DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): implications for hybrid detection

Published online by Cambridge University Press:  20 January 2017

Mark R. Jeschke ,
Patrick J. Tranel  and
A. Lane Rayburn
Mark R. Jeschke
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
A. Lane Rayburn
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
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Abstract

Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations.

Keywords

Smooth pigweed, Amaranthus hybridus L. AMACHtall waterhemp, Amaranthus tuberculatus (Moq.) Sauer AMATUDNA contentflow cytometryhybridizationintraspecific variation
Type
Research Article
Information
Weed Science , Volume 51 , Issue 1 , February 2003 , pp. 1 - 3
Copyright
Copyright © Weed Science Society of America 

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