Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-22T19:54:04.520Z Has data issue: false hasContentIssue false
  • English
  • Français

A detoxification gene in transgenic Nicotiana tabacum confers 2,4-D tolerance

Published online by Cambridge University Press:  12 June 2017

David I. Last  and
Danny J. Llewellyn
David I. Last
Affiliation:
CSIRO Division of Plant Industry, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

Transgenic Nicotiana tabacum with tolerance to 2,4-D has previously been produced using a bacterial 2,4-D-dioxygenase gene (tfdA) driven by the 35S promoter of cauliflower mosaic virus. Using promoters from the Pisum sativum plastocyanin gene (petE) and an Arabidopsis thaliana histone gene (H4A), we demonstrate that similar protection from 2,4-D can be obtained in transgenic N. tabacum by targeting expression of tfdA to either meristematic tissues or chloroplast-containing tissues. As with the 35S promoter constructs, the plants are tolerant but not completely resistant; very young seedlings in particular are only slightly protected. However, the levels of tolerance observed could offer a useful degree of protection from accidental spray drift.

Keywords

2,4-DNicotiana tabacum L., tobaccoPisum sativum L., peaCrop injuryherbicide resistancetargeted gene expressiontransgenic plantspromoter
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 47 , Issue 4 , August 1999 , pp. 401 - 404
Copyright
Copyright © 1999 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

An, G., Watson, B. D., Stachel, S., Cordon, M. P., and Nester, E. W. 1985. New cloning vehicles for transformation of higher plants. EMBO J. 4:277–284.Google Scholar
Atanassova, R., Chaubet, N., and Gigot, C. 1992. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis . Plant J. 2:291–300.CrossRefGoogle ScholarPubMed
Bayley, C., Trolinder, N., Ray, C., Morgan, M., Quisenberry, J. E., Ow, D. W. 1992. Engineering 2,4-D resistance in cotton. Theoret. Appl. Genet. 83:645–649.Google Scholar
Chaboute, M. E., Chaubet, N., Philipps, G., Ehling, M., and Gigot, C. 1987. Genomic organisation and nucleotide sequences of two histone H3 and two histone H4 genes in Arabidopsis thaliana . Plant Mol. Biol. 8:179–191.CrossRefGoogle Scholar
Charles, G. W. 1991. A grower survey of weeds and herbicide use in the New South Wales cotton industry. Aust. J. Exper. Agric. 31:387.Google Scholar
Chaudhury, A. M. and Signer, E. R. 1989. Non-destructive transformation of Arabidopsis . Plant Mol. Biol. Reporter 7:258–265.CrossRefGoogle Scholar
Don, R. H., Weightman, A. J., Knackmuss, H.-J., and Timmis, K. N. 1985. Transposon mutagenesis and cloning analysis of the pathway for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4). J. Bacteriol. 161:85–90.Google Scholar
Fukumori, F. and Hausinger, R. P. 1993. Alcaligenes eutrophus JMP134 “2,4-dichlorophenoxyacetate monooxygenase” is an α-ketoglutaratedependent dioxygenase. J. Bacteriol. 175:2083–2086.CrossRefGoogle Scholar
Guilley, H., Dudley, R. K., Jonard, G., Balazs, E., and Richards, K. E. 1982. Transcription of cauliflower mosaic virus DNA: detection of promoter sequences and characterization of transcripts. Cell 30:763–773.Google Scholar
Howard, E. A., Walker, J. C., Dennis, E. S., and Peacock, W. J. 1987. Regulated expression of an alcohol dehydrogenase 1 chimeric gene introduced into maize protoplasts. Planta 170:535–540.Google Scholar
Janssen, B-J. and Gardner, R. 1989. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium . Plant Mol. Biol. 14:61–72.Google Scholar
Jefferson, R. A., Kavanagh, T. A., and Bevan, M. W. 1987. GUS fusions: b-glucuronidase, a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901–3907.CrossRefGoogle Scholar
Kirschman, J. A. and Harris Cramer, J. 1988. Two new tools: multi-purpose cloning vectors that carry the kanamycin or spectinomycin/streptomycin resistance markers. Genet. 68:163–165.Google Scholar
Last, D. I. and Gray, J. C. 1989. Plastocyanin is encoded by a single-copy gene in the pea haploid genome. Plant Mol. Biol. 12:655–666.CrossRefGoogle ScholarPubMed
Lyon, B. R., Llewellyn, D. J., Huppatz, J. L., Dennis, E. S., and Peacock, W. J. 1989. Expression of a bacterial gene in transgenic tobacco confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid. Plant Mol. Biol. 13:533–540.CrossRefGoogle Scholar
Lyon, B. R., Cousins, Y., Llewellyn, D. J., Dennis, E. S. 1993. Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid. Transg. Res. 2:162–169.Google Scholar
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15:473–497.Google Scholar
Odell, J., Nagy, F., and Chua, N-H. 1985. Identification of DNA sequences required for the activity of a plant promoter: the CaMV promoter. Nature 313:810–812.Google Scholar
Perlak, F. J., Fuchs, R. L., Dean, D. A., McPherson, S. L., Fischoff, D. A. 1991. Modification of the coding sequence enhances plant expression of insect control protein genes. Proc. Natl. Acad. Sci. USA 88:3324–3328.CrossRefGoogle ScholarPubMed
Pwee, K.-H. and Gray, J. C. 1993. The pea plastocyanin promoter directs cell-specific but not full light regulated expression in transgenic tobacco. Plant J. 3:437–449.Google Scholar
Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Plainview, NY: Cold Spring Harbour Laboratory Press, pp. 1.2–1.110, 5.1–5.95.Google Scholar
Streber, W. R. and Willmitzer, L. 1989. Transgenic tobacco plants expressing a bacterial detoxifying enzyme are resistant to 2,4-D. Bio/Technology 7:811–816.Google Scholar
Vieira, J. and Messing, J. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153:3–11.Google Scholar