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Determination of Arsenical Herbicide Residues in Plant Tissues

Published online by Cambridge University Press:  12 June 2017

F. B. Anastasia
Affiliation:
Plant Sci. Lab., Fort Detrick, Frederick, Maryland
W. A. Wells
Affiliation:
Plant Sci. Lab., Fort Detrick, Frederick, Maryland

Abstract

Paper chromatographic separation of hydroxydimethylarsine oxide (cacodylic acid), monosodium methanearsonate (MSMA), sodium arsenate, and sodium arsenite was achieved with the aid of four solvent systems. Aqueous extracts of plant tissues removed essentially all the arsenicals applied, but methanolic fractionation was required before the extracts could be analyzed by paper chromatographic procedures. A standard nitric-sulfuric acid digestion procedure was employed for arsenic analyses, but great care was taken to avoid sulfuric-acid-induced charring by first adding relatively large amounts of nitric acid to drive off chlorides present. Depending upon the amount of chloride present, substantial losses of arsenic as arsine chlorides were observed if the samples charred. Five minutes in fuming sulfuric acid to completely break the carbon-arsenic bonds was another critical requirement for the quantitative determination of arsenic from cacodylic acid and MSMA. The silver diethyldithiocarbamate colorimetric method was useful for detecting as little as 0.6 μg or as much as 20 μg of arsenic per sample.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. American Conference of Government Industrial Hygienists. 1958. Manual of analytical methods–Determination of arsenic in air and biological materials. 1014 Broadway, Cincinnati, Ohio. 20 p.Google Scholar
2. Babko, A. K., Chalaya, Z. I., and Mikitchenko, V. F. 1966. The determination of microquantities of arsenic with butylrhodamine. (Transl. from Russian) Zavo. Lab. 32:270273.Google Scholar
3. Bruno, M. and Delluco, V. 1956. Separazione e dossaggio di microquantita di arsenico e di fosforo. Ric. Sci. 26:33373341.Google Scholar
4. Fischer, R. B. and Peters, D. G. 1968. Quantitative Chemical Analysis. 3d Edition. Saunders Co., Philadelphia. 883 p.Google Scholar
5. Miketukova, V., Kohlicek, J., and Kacl, K. 1968. Separation of arsenite and arsenate ions by paper chromatography. Study of a methanol-ammonia-water solvent system. J. Chromatogr. 34:284288.Google Scholar
6. Milton, R. F. and Waters, W. A. 1955. Methods of quantitative micro-analysis. 2d Edition. Edward Arnold, Ltd., London. 742 p.Google Scholar
7. Nelson, F. and Kraus, K. A. 1955. Anion-exchange studies. XVIII. Germanium and arsenic in HCl solutions. J. Amer. Chem. Soc. 77:45084509.Google Scholar
8. Oguma, K. 1967. The separation of arsenic (V) and (III) by thin-layer chromatography. Talanta 14:685687.Google Scholar
9. Overby, L. R., Bocchieri, S. F., and Frederickson, R. L. 1965. Chromatographic, electrophoretic and ion exchange identification of radioactive organic and inorganic arsenicals. J. Assoc. Offic. Agr. Chem. 48:1722.Google Scholar
10. Powers, G. W. Jr., Martin, R. L., Piehl, F. J., and Griffin, J. M. 1959. Arsenic in naphthas. Anal. Chem. 31:15891593.Google Scholar
11. Sachs, R. M. and Michael, J. L. 1971. Comparative phytotoxicity among four arsenical herbicides. Weed Sci. 19: (In Press).Google Scholar
12. Sckerl, M. M. and Frans, R. E. 1969. Translocation and metabolism of MAA−14C in Johnsongrass and cotton. Weed Sci. 17:421427.Google Scholar
13. Wilkinson, R. E. and Hardcastle, W. S. 1969. Plant and soil arsenic analyses. Weed Sci. 17:536537.Google Scholar