Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-25T21:22:42.375Z Has data issue: false hasContentIssue false

Cytological Effects of Ultra-High Temperatures on Corn

Published online by Cambridge University Press:  12 June 2017

T. C. Ellwanger Jr.
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
S. W. Bingham
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
W. E. Chappell
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
S. A. Tolin
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061

Abstract

Corn (Zea mays L. ‘Funk's G-83’) seedling leaves exposed to flame-generated ultra-high temperatures produced in flame cultivation were fixed in glutaraldehyde, post fixed in osmium tetroxide, and embedded in Araldite. In the light microscope, bundle sheath cells of flamed tissue were more heavily stained with Azure II and less vacuolated than were nonflamed cells. Heated mesophyll cells contained swollen, disrupted, and granular chloroplasts. Examination of flamed tissue by electron microscopy revealed granular, dispersed cytaplasm and altered membrane systems. Chloroplast lamellar systems and envelopes, tonoplasts, and plasmalemmas were disintegrated in both bundle sheath and mesophyll cells.

Type
Research Article
Copyright
Copyright © 1973 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Alexandrov, V. Y. 1964. Cytophysiological and cytoecological investigations of heat resistance of plant cells toward the action of high and low temperature. Quar. Rev. Biol. 39:3577.Google Scholar
2. Boyer, J. S. 1970. Differing sensitivity of photosynthesis to low leaf water potentials in corn and soybean. Plant Physiol. 46:236239.Google Scholar
3. Daniell, J. W., Chappell, W. E., and Couch, H. B. 1969. Effect of sublethal and lethal temperatures on plant cells. Plant Physiol. 44:16841689.Google Scholar
4. Edwards, F. E. 1964. History and progress of flame cultivation. Natural Gas Processors Assoc. Annu. Symp. Proc. 1:36.Google Scholar
5. Ellwanger, T. C., Bingham, S. W., and Chappell, W. E. 1973. Physiological effects of ultra-high temperatures on corn. Weed Sci. 21: (this issue).Google Scholar
6. Esau, K. 1965. Plant Anatomy. John Wiley and Sons, Inc., New York. 767 p.Google Scholar
7. Jeon, K. W. 1965. Simple method for staining and preserving epoxy resin embedded animal tissue sections for light microscopy. Life Sci. 4:18391841.Google Scholar
8. Luft, J. H. 1961. Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9:409414.Google Scholar
9. Rabinowitch, E. I. 1956. Photosynthesis, Volume II, Part 2, Interscience Publishers, Inc., New York. 877 p.Google Scholar
10. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17:208212.Google Scholar
11. Vanderkooi, G. and Green, D. 1971. New insights into biological membrane structure. Bioscience 21:409415.Google Scholar
12. Woo, K. C., Anderson, J. M., Boardman, N. K., Downton, W. J. S., Osmund, C. B., and Thorne, S. W. 1970. Deficient photosystem II in agranal bundle sheath chloroplasts of c4 plants. Proc. Nat. Acad. Sci. U.S.A. 67:1825.Google Scholar