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A Cucumber Bioassay Test for the Soil Residues of Certain Herbicides

Published online by Cambridge University Press:  12 June 2017

Clyde C. Dowler
Clyde C. Dowler*
Affiliation:
Crops Research Division, Agr. Res. Serv., U. S. Dep. of Agr., Federal Experiment Station, Mayaguez, Puerto Rico
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Abstract

A bioassay standard curve injury rating, using cucumbers (Cucumis sativus L.) as the indicator plant, was developed for quantitatively determining soil residues of 5-bromo-3-sec-butyl-6-methyluracil (bromacil), 6-dichloro-o-anisic acid (dicamba), 3(3,4-dichlorophenyl)-1,1-dimethylurea (diuron), (2,3,6-trichlorophenyl)-acetic acid (fenac), 4-amino-3,5,6-trichloropicolinic acid (picloram), and 2,4-bis(isopropylamino)-6-methoxy-s-triazine (prometone). The herbicide concentrations ranged from 0.001 to 2.187 ppmw. The presence of all the herbicides could be detected at 0.009 ppmw and the herbicides killed cucumbers at 2.187 ppmw. Cucumbers could be used to effectively measure a greater concentration range of fenac than the other herbicides. A highly significant negative correlation existed between injury rating and fresh plant weight. However, concentrations of the herbicides were more easily detected by a visual injury rating on abnormal growth characteristics than by fresh plant weight.

Type
Research Article
Information
Weed Science , Volume 17 , Issue 3 , July 1969 , pp. 309 - 310
Copyright
Copyright © 1969 Weed Science Society of America 

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References

Literature Cited

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