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Comparison of Chlorophyll Fluorescence and Fresh Weight as Herbicide Bioassay Techniques

Published online by Cambridge University Press:  12 June 2017

David R. Shaw ,
Thomas F. Peeper  and
D. L. Nofziger
David R. Shaw
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
Thomas F. Peeper
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
D. L. Nofziger
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
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Abstract

The sensitivities of chlorophyll fluorescence and fresh weight as bioassay techniques for the determination of metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one], diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], and atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] concentrations in soil were compared. The ratio of the initial inflection point (I) to the variable fluorescence maximum (P) of intact oat (Avena sativa L. ‘Chilocco’) leaves was determined for plants seeded directly into herbicide-treated soil and for those transplanted into treated soil after 14 days of growth in nutrient solution. Using the chlorophyll fluorescence of transplanted oats bioassay, 0-, 0.13-, 0.25-, and 0.50-ppm concentrations could be distinguished from one another within 8 h for metribuzin, within 24 h for diuron, and within 48 h for atrazine. These distinctions between rates could not be made 17 days after seeding into treated soil when using fresh weight as the bioassay indicator. Chlorophyll fluorescence of oats seeded directly into treated soil was also a reliable technique, but required much more time to attain sufficient plant size for convenient chlorophyll fluorescence determinations.

Keywords

Avena sativametribuzin, diuronatrazine
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 33 , Issue 1 , January 1985 , pp. 29 - 33
Copyright
Copyright © 1985 by the Weed Science Society of America 

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References

Literature Cited

1. Ahrens, W. H., Arntzen, C. J., and Stoller, E. W. 1981. Chlorophyll fluorescence assay for the determination of triazine resistance. Weed Sci. 29:316–322.CrossRefGoogle Scholar
2. Bowes, J., Crofts, A. R., and Arntzen, C. J. 1980. Redox reactions on the reducing side of photosystem II in chloroplasts with altered herbicide binding properties. Arch. Biochem. Biophys. 200:303–308.CrossRefGoogle ScholarPubMed
3. Brewer, P. E., Arntzen, C. J., and Slife, F. W. 1979. Effects of atrazine, cyanazine, and procyazine on the photochemical reactions of isolated chloroplasts. Weed Sci. 37:300–308.CrossRefGoogle Scholar
4. Devlin, R. M., Murkowski, A. J., Zbiec, I. I., Karczmarczyk, S. J., and Skorska, E. M. 1983. Influence of buthidazole, diuron, and atrazine on some light reactions of photosynthesis. Weed Sci. 31:879–883.CrossRefGoogle Scholar
5. Etienne, A. L., Lemasson, C., and Lavorel, J. 1974. Quenching de la chlorophylle in vivo par le m-nitrobenzene. Biochim. Biophys. Acta 33:288–300.CrossRefGoogle Scholar
6. Fischer, M. L. 1983. Investigations on the differential tolerance of wheat cultivars to metribuzin. Ph.D. Diss., Okla. State Univ., Diss. Abstr. Int. 44:2033.Google Scholar
7. Forbush, B. and Kok, B. 1968. Reaction between primary and secondary electron acceptors of photosystem II of photosynthesis. Biochim. Biophys. Acta 162:243–253.CrossRefGoogle ScholarPubMed
8. Hoagland, D. R. and Arnon, D. I. 1950. The water culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ. 547.Google Scholar
9. Marriage, P. B. 1975. Detection of triazine and urea herbicide residues by various characteristics of oat seedling in bioassays. Weed Res. 15:291–298.CrossRefGoogle Scholar
10. Miles, C. D. and Daniels, D. J. 1973. A rapid screening technique for photosynthetic mutants of higher plants. Plant Sci. Lett. 1:227–240.CrossRefGoogle Scholar
11. Murata, N., Nishimura, M., and Takamiya, A. 1966. Fluorescence of chlorophyll in photosynthetic systems. Biochim. Biophys. Acta. 120:23–33.CrossRefGoogle ScholarPubMed
12. Papageorgiou, G. 1975. Chlorophyll fluorescence: an intrinsic probe of photosynthesis. Pages 319–371 in Govindjee, , ed. Bioenergetics of Photosynthesis. Academic Press, New York.CrossRefGoogle Scholar
13. Richard, E. P. Jr., Goss, J. R., Arntzen, C. J., and Slife, F. W. 1983. Determination of herbicide inhibition of photosynthetic electron transport by fluorescence. Weed Sci. 31:361–367.CrossRefGoogle Scholar
14. Schreiber, U., Groberman, L., and Vidaver, W. 1975. Portable, solid-state fluorometer for the measurement of chlorophyll fluoresence induction in plants. Rev. Sci. Instrum. 46:538–542.CrossRefGoogle Scholar
15. Schreiber, U., Vidaver, W., Runeckles, V. C., and Rosen, P. 1978. Chlorophyll fluorescence assay for ozone injury in intact plants. Plant Physiol. 61:80–84.CrossRefGoogle ScholarPubMed
16. Sheets, T. J., Crafts, A. S., and Drever, H. R. 1962. Influence of soil properties on the phytotoxicity of the s-triazine herbicides. J. Agric. Food Chem. 10:458–462.CrossRefGoogle Scholar
17. Smillie, R. M. and Nott, R. 1982. Salt tolerance in crop plants monitored by chlorophyll fluorescence in vivo . Plant Physiol. 70:1049–1054.CrossRefGoogle ScholarPubMed
18. Van Assche, C. J. and Carles, P. M. 1982. Photosystem II inhibiting chemicals. Pages 1–21 in Moreland, D. E., St. John, J. B., and Hess, F. D., eds. Biochemical Responses Induced by Herbicides. Am. Chem. Soc., Washington, DC.Google Scholar