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Cell Membrane Permeability and Ultrastructural Effects of Difenzoquat on Wild Oats (Avena fatua)

Published online by Cambridge University Press:  12 June 2017

Kiet M. Thai ,
Sakti Jana  and
Larry C. Fowke
Kiet M. Thai
Affiliation:
Crop Sci. Plant Ecol. Dep., Univ. Sask., Saskatoon, Sask., Canada S7N OWO
Sakti Jana
Affiliation:
Crop Sci. Plant Ecol. Dep., Univ. Sask., Saskatoon, Sask., Canada S7N OWO
Larry C. Fowke
Affiliation:
Biol. Dep., Univ. Sask., Saskatoon, Sask., Canada S7N OWO
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Abstract

Effects of difenzoquat on wild oats grown under controlled environmental conditions were studied. Seedling height and fresh weight were significantly reduced 5 days after postemergence treatment. Dose-dependent increase in cell membrane permeability was detected after a 12-h exposure to the herbicide. Scanning electron micrographs showed normal leaf hairs and cuticular wax but swollen guard cells 10 days after treatment. Ultrastructural changes occurred before the visible symptoms. The primary effect of difenzoquat appears to be the disruption of the tonoplast and plasmalemma. The tonoplast showed greater damage than the plasmalemma. Secondary effects included damage to mitochondria and chloroplasts. Mitochondria were swollen and often ruptured, but the effect did not increase with the duration of exposure. Chloroplasts became spherical in shape, and their contents were also affected. The changes included: accumulation and then disappearance of starch granules, swelling of frets, fusion of granal thylakoids, detachment and then rupture of the outer membrane of the envelope, and clumping of ribosomes. By contrast, natural senescence caused greater injury of the plasmalemma than the tonoplast, a marked increase in size of plastoglobuli, and loss of starch grains without early accumulation.

Keywords

Difenzoquat, 1,2-dimethyl-3,5-diphenyl-1H-pyrazoliumwild oats, Avena fatua L. # AVEFAElectrolyte leakageelectron microscopynatural senescenceAVEFA
Type
Weed Control and Herbicide Technology
Information
Weed Science , Volume 37 , Issue 1 , January 1989 , pp. 98 - 106
Copyright
Copyright © 1989 by the Weed Science Society of America 

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References

Literature Cited

1. Anderson, J. L. and Schaelling, J. P. 1970. Effects of pyrazon on bean chloroplast ultrastructure. Weed Sci. 18:455–459.Google Scholar
2. Anderson, J. L. and Thomson, W. W. 1973. The effects of herbicides on the ultrastructure of plant cells. Residue Rev. 47:167–189.Google Scholar
3. Ashton, F. M., Bisalputra, T., and Risley, E. B. 1966. Effect of atrazine on Chlorella vulgaris . Am. J. Bot. 52:217–219.Google Scholar
4. Balke, N. E. 1985. Herbicide effects on membrane functions. Pages 113–140 in Duke, S. O., ed. Weed Physiology, Vol. 2, Herbicide Physiology. CRC Press, Boca Raton.Google Scholar
5. Bartels, P. G. 1985. Effects of herbicides on chloroplast and cellular development. Pages 64–90 in Duke, S. O., ed. Weed Physiology, Vol. 2, Herbicide Physiology. CRC Press, Boca Raton.Google Scholar
6. Brezeanu, A. G., Davis, D. G., and Shimabukuro, R. H. 1976. Ultrastructural effects and translocation of methyl-2-(4-(2,4-dichlorophenoxy)phenoxy) propanoate in wheat (Triticum aestivum) and wild oat (Avena fatua). Can. J. Bot. 54:2038–2048.Google Scholar
7. Butler, R. D. and Simon, E. W. 1971. Ultrastructural aspects of senescence in plants. Adv. Gerontol. Res. 3:73–129.Google Scholar
8. Castelfranco, P. and Bisalputra, T. 1965. Inhibitory effect of amitrole on Scenedesmus quadricauda . Am. J. Bot. 52:222–227.Google Scholar
9. Chow, P.N.P. 1982. Wild oat (Avena fatua) herbicide studies: I. Physiological response of wild oat to five postemergence herbicides. Weed Sci. 30:1–6.Google Scholar
10. DeKock, P. C. and Innes, A. M. 1970. The effect of amitrole on duckweed. Can. J. Bot. 48:1285–1288.Google Scholar
11. Friesen, H. A. and Litwin, O. B. 1975. Selective control of wild oats in barley with AC 84777. Can. J. Plant Sci. 55:927–934.Google Scholar
12. Harris, N. and Dodge, A. D. 1972a. The effect of paraquat on flax cotyledon leaves: Changes in fine structure. Planta 104: 201–209.Google Scholar
13. Harris, N. and Dodge, A. D. 1974b. The effect of paraquat on flax cotyledon leaves: Physiological and biochemical changes. Planta 104:210–219.Google Scholar
14. Linck, A. J. 1971. Effect on cytology and fine structure of plant cells. Pages 83–125 in Audus, L. M., ed. Herbicides: Physiology, Biochemistry, Ecology. Vol. 1. Academic Press, New York.Google Scholar
15. Pallet, K. E. 1982. The contact activity of difenzoquat in two United Kingdom spring wheat cultivars. Weed Res. 22:329–335.Google Scholar
16. Pallet, K. E. and Caseley, J. C. 1980. Differential inhibition of DNA synthesis in difenzoquat tolerant and susceptible United Kingdom spring wheat cultivars. Pestic. Biochem. Physiol. 14:144–152.Google Scholar
17. Prendeville, G. N. and Warren, G. F. 1977. Effect of four herbicides and two oils on leaf-cell membrane permeability. Weed Res. 30:251–258.Google Scholar
18. Thai, K. M., Jana, S., and Naylor, J. M. 1985. Variability for response to herbicides in wild oat (Avena fatua) populations. Weed Sci. 33:829–835.Google Scholar
19. Vanstone, D. E. and Stobbe, E. H. 1977. Electrolytic conductivity – a rapid measure of herbicide injury. Weed Sci. 25:352–354.Google Scholar