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Cell Cycle Population Kinetics of Pea Root Tip Meristems Treated with Propham

Published online by Cambridge University Press:  12 June 2017

Thomas L. Rost  and
David E. Bayer
Thomas L. Rost
Affiliation:
Dep. Bot., Univ. of California, Davis, CA 95616
David E. Bayer
Affiliation:
Dep. Bot., Univ. of California, Davis, CA 95616
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Abstract

Propham (Isopropyl carbanilate) at 10-5 M concentration does not inhibit DNA synthesis nor does it reduce the number of dividing cells. It does, however, induce a number of abnormal mitotic figures. At higher concentrations (10-3 M) propham inhibits DNA, RNA, and protein synthesis as well as stopping entry of cells into mitosis and causing those which divide to be aberrant. DNA and RNA synthesis are not inhibited for approximately 3 hr, while protein synthesis is reduced within 1 hr. The metabolic inhibitory effect of propham is reversible at least in roots treated for 8 hr. The most likely mode of action of propham is the inhibition of certain mitotic specific proteins, or possibly some kind of protein binding function as has been suggested in the literature.

Type
Research Article
Information
Weed Science , Volume 24 , Issue 1 , January 1976 , pp. 81 - 87
Copyright
Copyright © 1976 by the Weed Science Society of America 

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References

Literature Cited

1. Ashton, F.M., Penner, D., and Hoffman, S. 1968. Effect of several herbicides on proteolytic activity on squash seedlings. Weed Sci. 16:169–171.Google Scholar
2. Bartels, P.G. and Hilton, J.L. 1973. Comparison of trifluralin, oryzalin, pronamide, propham and colchicine treatments on microtubules. Pest. Biochem. Physiol. 3:462–472.Google Scholar
3. Briquet, M.V. and Wiaux, A.L. 1967. Herbicides and RNA synthesis of Pisum and Avena roots. Meded. Rijksfac. LandbWet. Gent, 32:1040–1049.Google Scholar
4. Canvin, D.T. and Friesen, G. 1959. Cytological effects of CDAA and IPC on germinating barley and peas. Weeds 7:153–156.Google Scholar
5. Ennis, W.B. Jr. 1948. Some cytological effects of O-Isopropyl N-Phenyl carbamate upon Avena . Amer. J. Bot. 35:15–21.Google Scholar
6. Hepler, P.K. and Jackson, W.T. 1969. Isopropyl N-phenyl-carbamate affects spindle microtubule orientation in dividing endosperm cells of Haemanthus hatherinae . J. Cell Sci. 5:727–743.CrossRefGoogle Scholar
7. Howard, A. and Pelc, S.R. 1953. Synthesis of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity Suppl. 6:261–273.Google Scholar
8. Keitt, G.W. Jr. 1967. On the method of action of carbamate herbicides. Physiol. Plant. 20:1076–1082.Google Scholar
9. Kihlman, B.A. 1966. Actions of chemicals on dividing cells. Prentice-Hall, Inc. Engleman Cliffs, N.J. 260 pp.Google Scholar
10. Mann, J.D., Jordan, L.S., and Day, B.E. 1965. The effects of carbamate herbicides on polymer synthesis. Weeds 13:63–66.Google Scholar
11. Mann, J.D., Jordan, L.S., and Day, B.E. 1965. A survey of herbicides for their effect upon protein synthesis. Plant Physiol. 40:840–843.Google Scholar
12. Mann, J.D., Cota-Robles, E., Yung, K-H., Pu, M., and Haid, H. 1967. Phenylurethane herbicides: Inhibitors of changes in metabolic state. I. Botanical aspects. Biochim. Biophys. Acta 138:133–139.Google Scholar
13. Mann, J.D. and Storey, W.B. 1966. Rapid action of carbamate herbicides upon plant cell nuclei. Cytologia 31:203–207.CrossRefGoogle Scholar
14. Mazia, D. 1974. The cell cycle. Sci. Amer. 230:54–64.Google Scholar
15. Mitchison, J.M. 1971. Page 201 in The biology of the cell cycle. Cambridge Univ. Press.Google Scholar
16. Moreland, D.E., Malhotra, S.S., Gruenhagen, R.D., and Shokrah, E.H. 1969. Effects of herbicides on RNA and protein synthesis. Weed Sci. 17:556–563.Google Scholar
17. Rost, T.L. and Van't Hof, J. 1973. The comparative radio-sensitivity, RNA and protein metabolism of cells inhibited and uninhibited from DNA synthesis in sucrose deficient cultures of sunflower root meristems (Helianthus annum). Amer. J. Bot 60:172–181.Google Scholar
18. Van't Hof, J. 1966. Experimental control of DNA synthesizing and dividing cells in excised root tips of Pisum . Amer. J. Bot. 53:970–976.Google Scholar
19. Van't Hof, J. and Kovacs, C.J. 1972. Mitotic cycle regulation in the meristem of cultured roots: The principal control point hypothesis. (from Conf. The dynamics of meristem cell populations to be in Advances in Experimental Medicine and Biology. Plenum Press.).Google Scholar
20. Webster, P.L. and Van't Hof, J. 1970. DNA synthesis and mitosis in meristems: Requirements for RNA and protein synthesis. Amer. J. Bot. 57:130–139.Google Scholar
21. White, P.R. 1943. A handbook of plant tissue culture. Cattell, Lancaster, PA. 277 pp.Google Scholar
22. Wilson, G.B. 1966. Cell division and the mitotic cycle. Reinhold Pub. Corp. N.Y. 111 pp.Google Scholar