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Bromoxynil resistance in transgenic potato clones expressing the bxn gene

Published online by Cambridge University Press:  12 June 2017

Charlotte V. Eberlein ,
Mary J. Guttieri  and
Jody Steffen-Campbell
Mary J. Guttieri
Affiliation:
University of Idaho, Aberdeen, ID 83210
Jody Steffen-Campbell
Affiliation:
USDA/ARS Western Regional Research Center, Albany, CA 94710
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Abstract

To broaden the spectrum of herbicides useful in potato production, the bxn gene for bromoxynil resistance, which encodes a nitrilase specific for bromoxynil, was introduced into ‘Lemhi Russet’ potato by Agrobacterium tumefaciens-mediated transformation. In GR50 studies, transformed potato clones were at least 70-fold more resistant to bromoxynil than the untransformed control. Resistance was due to rapid metabolism of bromoxynil to 3,5-dibromo-4-hydroxybenzoic acid, followed by conjugation to polar compounds. In yield trials, the best performing transgenic clones had total tuber yields equal to the untreated, untransformed control, but U.S. No. 1 tuber yields were 15 to 30% lower than the untreated, untransformed control. Tubers from three out of four transgenic clones had specific gravities, percent solids, and fry color similar to or better than the untreated, untransformed control. The data suggest that lower U.S. No. 1 yields in the transgenic clones were due to somaclonal variation and that expression of the bxn transgene had no consistent, detrimental effect on internal tuber quality.

Keywords

Bromoxynil, 3,5-dibromo-4-hydroxybenzonitrilepotato, Solanum tuberosum L. cv. Lemhi RussetGenetic engineeringherbicide resistancemetabolismnitrilase
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 46 , Issue 2 , April 1998 , pp. 150 - 157
Copyright
Copyright © 1998 by the Weed Science Society of America 

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References

Literature Cited

Ahrens, W. H., ed. 1994. Herbicide Handbook. 7th ed. Champaign, IL: Weed Science Society of America. 352 p.Google Scholar
An, G., Watson, B. D., Stachel, S., Gordon, M. P., and Nester, E. W. 1985. New cloning vehicles for transformation of higher plants. EMBO J. 4: 277–284.Google Scholar
Belknap, W. R., Corsini, D., Pavek, J. J., Snyder, G. W., and Rockhold, D. R. 1994a. Field performance of transgenic potatoes. Pages 233–244 in Belknap, W. R., Vayda, M. E., and Park, W. D., eds. Molecular and Cellular Biology of the Potato. 2nd ed. Wallingford: C.A.B. International.Google Scholar
Belknap, W. R., Corsini, D., Pavek, J. J., Snyder, G. W., Rockhold, D. R., and Vayda, M. E. 1994b. Field performance of transgenic Russet Burbank and Lemhi Russet potatoes. Am. Potato J. 71: 285–296.Google Scholar
Bevan, M., Barnes, W. M., and Chilton, M. D. 1983. Structure and expression of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 11: 369–385.Google Scholar
Buckland, J. L., Collins, R. F., and Pullin, E. M. 1973. Metabolism of bromoxynil octanoate in growing wheat. Pestic. Sci. 4: 149–162.Google Scholar
Chang, H. H. and Chan, M. T. 1991. Improvement of potato (Solanum tuberosum L.) transformation by Agrobacterium in the presence of silver thiosulfate. Bot. Bull. Acad. Sci. 32: 63–70.Google Scholar
Eberlein, C. V., Guttieri, M. J., and Fletcher, F. E. 1993. Broadleaf weed control in potatoes (Solanum tuberosum) with postemergence directed herbicides. Weed Technol. 7: 298–303.CrossRefGoogle Scholar
Feldman, K. A. 1991. T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum. Plant J. 1: 71–82.CrossRefGoogle Scholar
Garbarino, J. E., Rockhold, D. R., and Belknap, W. R. 1992. Expression of stress-responsive ubiquitin genes in potato tubers. Plant Mol. Biol. 20: 235–244.Google Scholar
Haderlie, L. C. and Poulson, M. 1991. 1990 Weed Control Survey. Proc. Univ. Idaho Winter Commodity Schools 23: 149–154.Google Scholar
Hoekema, A., Hirsch, P. R., Hooykaas, P.J.J., and Schilperoort, R. A. 1983. A binary plant vector strategy based on separation of the vir- and T-region of Agrobacterium tumefaciens Ti plasmid. Nature 303: 179–180.Google Scholar
Kleinschmidt, G. D., Kleinkopf, G. E., Westermann, D. T., and Zalewski, J. C. 1984. Specific Gravity of Potatoes. Moscow, ID: University of Idaho CIS No. 609.Google Scholar
McBride, K. E., Kenny, J. W., and Stalker, D. M. 1986. Metabolism of the herbicide bromoxynil by Klebsiella pneumoniae subsp. ozaenae. Appl. Environ. Microbiol. 52: 325–330.Google Scholar
McBride, K. E. and Summerfelt, K. 1990. Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 14: 269–276.Google Scholar
Rickey, T. M. and Belknap, W. R. 1991. Comparison of the expression of several stress responsive genes in potato tubers. Plant Mol. Biol. 16: 1009–1018.Google Scholar
Rietveld, R. C., Hasegawa, P. M., and Bressan, R. A. 1991. Somaclonal variation in tuber disc-derived populations of potato. I. Evidence of genetic stability across tuber generations and diverse locations. Theor. Appl. Genet. 82: 430–440.Google Scholar
Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular Cloning. 2nd ed. Plainview: New York: Cold Spring Harbor Laboratory Press. pp. 1.25–1.85.Google Scholar
Snyder, G. W. and Belknap, W. R. 1993. A modified method for routine Agrobacterium-mediated transformation of in vitro grown potato microtubers. Plant Cell Rep. 12: 324–327.Google Scholar
Stalker, D. M., Kiser, J. A., Baldwin, G., Coulombe, B., and Houck, C. M. 1996. Cotton weed control using the bxn system. Pages 93–105 in Duke, S. O., ed. Herbicide Resistant Crops. Boca Raton, FL: CRC Press.Google Scholar
Stalker, D. M., Malyj, L. D., and McBride, K. E. 1988a. Purification and properties of a nitrilase specific for the herbicide bromoxynil and corresponding nucleotide sequence analysis of the bxn gene. J. Biol. Chem. 263: 6310–6314.Google Scholar
Stalker, D. M., McBride, K. E., and Malyj, L. D. 1988b. Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science 242: 419–423.Google Scholar
Thimann, K. V. and Mahadevan, S. 1964. Nitrilase. I. Occurrence, preparation, and general properties of the enzyme. Arch. Biochem. Biophys. 105: 133–141.Google Scholar
Verwoerd, T. C., Dekker, B.M.M., and Hoekema, A. 1989. A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 17: 2362.Google Scholar