Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-22T19:46:13.901Z Has data issue: false hasContentIssue false
  • English
  • Français

Biological and biochemical detection techniques for glufosinate

Published online by Cambridge University Press:  20 January 2017

Xiao-Hui Zhou  and
Ching-Yuh Wang
Xiao-Hui Zhou
Affiliation:
Department of Agronomy, National Chung-Hsing University, 250 Kuokuang Road, Taichung, Taiwan
Get access Rights & Permissions [Opens in a new window]

Abstract

Biological and biochemical methods, based on glufosinate inhibitory effects on plant growth and nitrogen metabolism, were examined for their applications to detect this herbicide. Dose–response analysis of radicle growth inhibition showed that, among six vegetables tested, Chinese mustard and edible amaranth were the most sensitive to glufosinate. Field mustard and cruciferous Ching-Geeng were found to be more sensitive to this herbicide than the other four vegetables when three-leaf seedlings were tested in another bioassay. In three-leaf seedlings of Ching-Geeng, accumulation of ammonium, a biochemical marker for glufosinate toxicity because of its inhibition of glutamine synthetase, showed a linear regression to the log-transformed concentrations of glufosinate ranging from 7.5 × 10−5 to 1.5 × 10−3 M. For the detection of glufosinate lower than 7.5 × 10−6 M, a linear regression was observed between ammonium accumulation and the applied concentration, instead of the log-transformed value, of glufosinate. A similar relationship was observed between the accumulation in Ching-Geeng seedlings of glyoxylate, another biochemical marker, and glufosinate but with a narrower range than that for ammonium accumulation. The applicability of ammonium accumulation in three-leaf seedlings of Ching-Geeng to detect glufosinate residue in water and soil was confirmed by high-performance liquid chromatography (HPLC).

Keywords

GlufosinateChinese mustard, Brassica rapa L.field mustard, Brassica campestris L.cabbage, Brassica oleracea L. var. CapitataChing-Geeng, Brassica chinensis L., ‘Ching-Geeng’edible amaranth, Amaranthus tricolor L.Bioassaybiochemical markerherbicide detectionherbicide persistence
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 54 , Issue 3 , June 2006 , pp. 413 - 418
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

AGBIOS. 2006. GM database: Information on Genetically Modified Approved Products. http://64.26.159.139/dbase.php.Google Scholar
Barry, J. S., Swanton, C. J., Mersey, B. G., and Hall, J. C. 1992. Glufosinate (phosphinothricin) inhibition of nitrogen metabolism in barley and green foxtail plants. J. Plant Physiol 139:605–610.Google Scholar
Bradshaw, L. D., Barrett, M., and Poneleit, C. G. 1994. Inheritance of bentazon susceptibility in a corn (Zea mays) line. Weed Sci 42:641–647.Google Scholar
Coetzer, E. and Al-Khatib, K. 2001. Photosynthetic inhibition and ammonium accumulation in Palmer amaranth after glufosinate application. Weed Sci 49:454–459.Google Scholar
D'Halluin, K., DeBlock, M., Janssens, J., Leemans, J., Reynaerts, A., and Botterman, J. 1992. The bar gene as a selectable marker in plant engineering. Method. Enzymol 216:415–441.CrossRefGoogle ScholarPubMed
Fauci, M., Bezdicek, D. F., Caldwell, D., and Finch, R. 2002. Development of plant bioassay to detect herbicide contamination of compost at or below practical analytical detection limits. Bull. Environ. Contam. Toxicol 68:79–85.Google Scholar
Gallina, M. A. and Stephenson, G. R. 1992. Dissipation of [14C]glufosinate ammonium in two Ontario soils. J. Agric. Food Chem 40:165–168.CrossRefGoogle Scholar
Gonzalez-Moro, B., Lacuesta, M., Begerril, J. M., Gonzalez-Murua, C., and Muñoz- Rueda, A. 1997. Glycolate accumulation causes a decrease of photosynthesis by inhibiting RUBISCO activity in maize. J. Plant Physiol 150:388–394.Google Scholar
Gonzalez-Moro, B., Lacuesta, M., Royuela, M., Muñoz-Rueda, A., and Gonzalez- Murua, C. 1993. Comparative study of the inhibition of photosynthesis caused by aminooxyacetic acid and phosphinothricin in Zea mays . J. Plant Physiol 142:161–166.Google Scholar
Hori, Y., Fujisawa, M., Shimada, K., Sato, M., Honda, M., and Hirose, Y. 2002. Enantioselective analysis of glufosinate using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate and reverse-phase liquid chromatography. J. Chromatogr. B 776:191–198.CrossRefGoogle Scholar
Ismail, B. S. and Ahmad, A. R. 1994. Attenuation of the herbicidal activities of glufosinate-ammonium and imazapyr in two soils. Agric. Ecosys. Environ 47:279–285.Google Scholar
Kataoka, H., Ryu, S., Sakiyama, N., and Mslita, M. 1996. Simple and rapid determination of the herbicides glyphosate and glufosinate in river water, soil and carrot samples by gas chromatography with flame photometric detection. J. Chromatogr. A 726:253–258.CrossRefGoogle Scholar
Li, Y. Z. and Wang, C. Y. 2005. 2-aminobutyric acid as a chemical marker for the detection of sulfonylurea herbicide. Weed Technol 19:176–182.CrossRefGoogle Scholar
Petrarulo, M., Pellegrino, S., Bioanco, O., Marangella, M., and Linari, F. 1988. High-performance liquid chromatography determination of glyoxylic acid in urine. J. Chromatogr. A 432:97–46.Google Scholar
Sancho, J. V., Hernańdez, F., López, F. J., Hogendoorn, E. A., Dijkman, E., and van Zoonen, P. 1996. Rapid determination of glufosinate, glyphosate and aminomethyl-phosphonic acid in environmental water samples using precolumn fluorogenic labeling and coupled-column liquid chromatography. J. Chromatogr. A 737:75–83.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicides dose–response relationship. Weed Technol 9:218–227.Google Scholar
Seelye, J. F., Borst, W. M., King, G. A., Hannan, P. J., and Maddocks, D. 1995. Glutamine synthetase activity, ammonium accumulation and growth of callus cultures of Asparagus officinalis L. exposed to high ammonium or phosphinothricin. J. Plant Physiol 146:686–692.Google Scholar
Sellers, B. A., Smeda, R. J., and Li, J. 2004. Glutamine synthetase activity and ammonium accumulation is influenced by time of glufosinate application. Pestic. Biochem. Physiol 78:9–20.CrossRefGoogle Scholar
Steckel, G. J., Wax, L. M., Simmons, F. W., and Phillips, W. H. 1997. Glufosinate efficacy on annual weeds is influenced by rate and growth stage. Weed Technol 11:484–488.Google Scholar
Wang, C. Y. 2002. Effect of glyphosate on tuber sprouting and growth of purple nutsedge (Cyperus rotundus L). Weed Technol 16:477–481.Google Scholar
Wendler, C., Putzer, M. A., and Wild, A. 1992. Effect of phosphinothricin (glufosinate) and inhibitors of photorespiration on photosynthesis and ribulose-1,5-bisphosphate carboxylase activity. J. Plant Physiol 139:666–671.Google Scholar
You, W. and Barker, A. V. 2002. Herbicidal action of root-applied glufosinate ammonium on tomato plants. J. Am. Soc. Hortic. Sci 127:200–204.Google Scholar