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Assessment of two nondestructive assays for detecting glyphosate resistance in horseweed (Conyza canadensis)

Published online by Cambridge University Press:  20 January 2017

Clifford H. Koger ,
Dale L. Shaner ,
W. Brien Henry ,
Talia Nadler-Hassar ,
Walter E. Thomas  and
John W. Wilcut
Dale L. Shaner
Affiliation:
USDA-ARS, Water Management Research Unit, 2150 Centre Avenue, Building D, Suite 320, Fort Collins, CO 80526
W. Brien Henry
Affiliation:
USDA-ARS, Central Plains Research Station, 40335 County Road GG, Akron, CO 80720
Talia Nadler-Hassar
Affiliation:
Colorado State University, Biological Science and Pest Management, Weed Science Lab, Fort Collins, CO 80527
Walter E. Thomas
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John W. Wilcut
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
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Abstract

Two rapid, nondestructive assays were developed and tested for their potential in differentiating glyphosate-resistant from glyphosate-susceptible biotypes of horseweed. In one assay, leaves of glyphosate-resistant and -susceptible corn, cotton, and soybean plants, as well as glyphosate-resistant and -susceptible horseweed plants, were dipped in solutions of 0, 300, 600, and 1,200 mg ae L−1 glyphosate for 3 d, and subsequent injury was evaluated. In the second assay, plant sensitivity to glyphosate was evaluated in vivo by incubating excised leaf disc tissue from the same plants used in the first assay in 0.7, 1.3, 2.6, 5.3, 10.6, 21.1, 42.3, and 84.5 mg ae L−1 glyphosate solutions for 16 h and measuring shikimate levels with a spectrophotometer. The leaf dip assay differentiated between glyphosate-resistant and -susceptible crops and horseweed biotypes. The 600 mg L−1 rate of glyphosate was more consistent in differentiating resistant and susceptible plants compared with the 300 and 1,200 mg L−1 rates. The in vivo assay detected significant differences between susceptible and glyphosate-resistant plants of all species. Shikimate accumulated in a glyphosate dose–dependent manner in leaf discs from susceptible crops, but shikimate did not accumulate in leaf discs from resistant crops, and levels were similar to nontreated leaf discs. Shikimate accumulated at high (≥ 21.1 mg ae L−1) concentrations of glyphosate in leaf discs from all horseweed biotypes. Shikimate accumulated at low glyphosate concentrations (≤ 10.6 mg L−1) in leaf discs from susceptible horseweed biotypes but not in resistant biotypes. Both assays were able to differentiate resistant from susceptible biotypes of horseweed and could have utility for screening other weed populations for resistance to glyphosate.

Keywords

Glyphosatehorseweed, Conyza canadensis (L.) Cronq. ERICAcorn, Zea mays L. ‘Dekalb 687RR’, ‘Pioneer 31B13’cotton, Gossypium hirsutum L. ‘Delta and Pine Land 444RR’, ‘Suregrow 747’soybean, Glycine max (L.) Merr. ‘Delta and Pine Land 4748’, ‘Asgrow 4702RR’Absorbancebioassayglyphosate resistanceherbicide resistanceleaf dipleaf discshikimate
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 53 , Issue 4 , August 2005 , pp. 438 - 445
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Baerson, S. R., Rodriguez, D. J., Tan, M., Feng, Y., Biest, N. A., and Dill, G. M. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:1265–1275.Google Scholar
Beckie, H. J., Heap, I. M., Smeda, R. J., and Hall, L. M. 2000. Screening for herbicide resistance in weeds. Weed Technol 14:428–445.Google Scholar
Blackshaw, R. E. and Harker, K. N. 2002. Selective weed control with glyphosate in glyphosate-resistant spring wheat (Triticum aestivum). Weed Technol 16:885–892.Google Scholar
Boutsalis, P. 2001. Syngenta quick-test: a rapid whole-plant test for herbicide resistance. Weed Technol 15:257–263.Google Scholar
Bresnahan, G. A., Manthey, F. A., Howatt, K. A., and Chakraborty, M. 2003. Glyphosate applied preharvest induces shikimic acid accumulation in hard red spring wheat (Triticum aestivum). J. Agric. Food Chem 51:4004–4007.CrossRefGoogle ScholarPubMed
Brunoehler, R. 2004. Gauging growth of glyphosate resistance. Corn Soybean Dig. February 15:23–24.Google Scholar
Chachalis, D., Reddy, K. N., Elmore, C. D., and Steele, M. L. 2001. Herbicide efficacy, leaf structure, and spray droplet contact angle among Ipomoea species and smallflower morningglory. Weed Sci 49:628–634.Google Scholar
Cromartie, T. H. and Polge, N. D. 2000. An improved assay for shikimic acid and its use as a monitor for the activity of sulfosate. Proc. Weed Sci. Soc. Am 40:291.Google Scholar
Escorial, M. C., Sixto, H., Gracia-Baudin, J. M., and Chueca, M. C. 2001. A rapid method to determine cereal plant response to glyphosate. Weed Technol 15:697–702.CrossRefGoogle Scholar
Faircloth, W. H., Patterson, M. G., Monks, C. D., and Goodman, W. R. 2001. Weed management programs for glyphosate-tolerant cotton (Gossypium hirsutum). Weed Technol 15:544–551.CrossRefGoogle Scholar
Feng, P. C. C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and CaJacob, C. A. 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498–505.CrossRefGoogle Scholar
Gower, S. A., Loux, M. M., and Cardina, J. et al. 2003. Effect of postemergence glyphosate application timing on weed control and grain yield in glyphosate-resistant corn: results of a 2-yr multistate study. Weed Technol 17:821–828.Google Scholar
Heap, I. 2004. The international survey of herbicide resistant weeds. www.weedscience.com.Google Scholar
Henry, W. B., Koger, C. H., and Shaner, D. L. 2004. Shikimate accumulation in conventional corn and soybean as affected by sub-lethal rates of glyphosate. Proc. Weed Sci. Soc. Am 44:27.Google Scholar
Hoagland, D. R. and Arnon, D. I. 1950. The water culture method for growing plant without soil. Berkley, CA: University of California, California Agricultural Experiment Station Circ. 347.Google Scholar
Koger, C. H., Poston, D. H., Hayes, R. M., and Montgomery, R. F. 2004a. Glyphosate-resistant horseweed (Conyza canadensis) in Mississippi. Weed Technol 18:820–825.Google Scholar
Koger, C. H., Poston, D. H., and Reddy, K. N. 2004b. Effect of glyphosate spray coverage on control of pitted morningglory (Ipomoea lacunosa). Weed Technol 18:124–130.CrossRefGoogle Scholar
Koger, C. H., Price, A. J., and Reddy, K. N. 2005. Weed control and cotton (Gossypium hirsutum) response to combinations of glyphosate and trifloxysulfuron. Weed Technol 19:113–121.CrossRefGoogle Scholar
Koger, C. H. and Reddy, K. N. 2005. Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci 53:84–89.Google Scholar
Lee, L. J. and Ngim, J. 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica (L.) Gaertn) in Malaysia. Pest Manag. Sci 56:336–339.Google Scholar
Lorraine-Colwill, D. F., Powles, S. B., Hawkes, T. R., Hollinshead, P. H., Warner, S. A. J., and Preston, C. 2003. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol 74:62–72.Google Scholar
Main, C. L., Pantalone, V. R., and Mueller, T. C. 2004. A novel approach to determine the glyphosate tolerant trait in soybeans. J. Agric. Food Chem 52:1224–1227.Google Scholar
Mueller, T. C., Massey, J. H., Hayes, R. M., Main, C. L., and Stewart, C. N. Jr. 2003. Shikimate accumulation in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq). J. Agric. Food Chem 51:680–684.CrossRefGoogle ScholarPubMed
Perez, A. and Kogan, M. 2003. Glyphosate-resistant Lolium multiflorum in Chilean orchards. Weed Res 43:12–19.Google Scholar
Pline, W. A., Wilcut, J. W., Duke, S. O., Edmisten, K. L., and Wells, R. 2002. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate-resistant and nonglyphosate-resistant cotton (Gossypium hirsutum L). J. Agric. Food Chem 50:506–512.Google Scholar
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrsss (Lolium rigidum) in Australia. Weed Sci 46:604–607.Google Scholar
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci 47:405–411.Google Scholar
Reddy, K. N. and Koger, C. H. 2005. Herbicide-resistant crops and weed management. Pages xx–xx in Singh, H. P. ed. Handbook of Sustainable Weed Management. Binghamton, NY: Haworth. In press.Google Scholar
[SAS] Statistical Analysis Systems. 1998. SAS/STAT User's Guide. Release 7.00. Cary, NC: Statistical Analysis Systems Institute. 1028 p.Google Scholar
Scott, G. H., Askew, S. D., and Wilcut, J. W. 2002. Glyphosate systems for weed control in glyphosate-tolerant cotton (Gossypium hirsutum). Weed Technol 16:191–198.Google Scholar
Seber, G. A. F. and Wild, C. J. 1989. Nonlinear Regression. New York: John Wiley. 768 p.Google Scholar
Shaner, D. L., Nadler-Hassar, T., Henry, W. B., and Koger, C. H. 2005. A rapid in vivo EPSPS assay with excised leaf discs. Weed Technol. In press.Google Scholar
Shaw, D. R. and Arnold, J. C. 2002. Weed control from herbicide combinations with glyphosate. Weed Technol 16:1–6.CrossRefGoogle Scholar
Simarmata, M. and Penner, D. 2004. Role of EPSP synthase in glyphosate resistance in rigid ryegrass (Lolium rigidum Gaud). Weed Sci. Soc. Am. Abstr 44:34.Google Scholar
Singh, B. J. and Shaner, D. E. 1998. Rapid determination of glyphosate injury to plants and identification of glyphosate-resistant plants. Weed Technol 12:527–530.Google Scholar
[USDA-NASS] U.S. Department of Agriculture–National Agricultural Statistics Service. 2004. Acreage. http://usda.mannlib.cornell.edu/reports/nassr/field/pcp-bba/acrg0604.pdf.Google Scholar
VanGessel, M. J. 2001. Glyphosate-resistant horseweed from Delaware. Weed Sci 49:703–705.Google Scholar
Wilcut, J. W. and Askew, S. D. 1999. Chemical weed control. Pages 627–661 in Ruberson, J. R. ed. Handbook of Pest Management. New York: Marcel Dekker.Google Scholar