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Aryloxyalkanoate Dioxygenase-12 Soybean Protein Expression

Published online by Cambridge University Press:  20 January 2017

Andrew P. Robinson ,
D. M. Simpson ,
Kerrm Yau ,
Sarah Canada  and
William G. Johnson
Andrew P. Robinson
Affiliation:
Department of Botany and Plant Pathology, 915 W. State Street, Purdue University, West Lafayette, IN 47907
D. M. Simpson
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
Kerrm Yau
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
Sarah Canada
Affiliation:
Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268
William G. Johnson*
Affiliation:
Department of Botany and Plant Pathology, 915 W. State Street, Purdue University, West Lafayette, IN 47907
*
Corresponding author's E-mail: [email protected]
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Abstract

New trait technology incorporating 2,4-D resistance in soybean is dependent upon the ability of the plant to metabolize 2,4-D by the aryloxyalkanoate dioxygenase-12 protein (AAD-12). Our objectives were to determine AAD-12 expression during the daytime, throughout the leaf canopy, and before and after 2,4-D treatment for the events DAS-68416-4 and DAS-21606-3. Field experiments were conducted near Wanatah, IN in 2009 and Fowler, IN in 2009, 2010, and 2011. During the daytime, total AAD-12 expression was lowest between 12:30 and 15:30, averaging 161 ng cm−2, as compared to an average of 245 ng cm−2 in the morning and 243 ng cm−2 in the evening. The youngest fully emerged trifoliate in the DAS-68416-4 event had the highest AAD-12 expression, with means ranging from 369 to 390 ng cm−2, while the older leaves maintained a lower level of expression, 171 to 211 ng cm−2. The youngest leaves of event DAS-21606-3 had the highest level of AAD-12 expression (205 to 225 ng cm−2), while the level of AAD-12 was lower in older leaves (71 to 149 ng cm−2). In general, 2,4-D treatments did not reduce AAD-12 expression at 3, 7, 14, and 21 days after treatment; however, in a few instances AAD-12 expression was increased or decreased by 8 to 11% after 2,4-D treatment. Expression of AAD-12 was between 152 to 390 ng cm−2 for DAS-68416-4 and from 71 to 244 ng cm−2 for DAS-21606-3.

Keywords

2,4-DGlycine max (L.) Merr., soybean.2,4-dichlorophenoxyacetic acid2,4-D-tolerant soybeantransgenic soybean
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 63 , Issue 1 , March 2015 , pp. 229 - 234
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Bruns, H, Craig, A (2003) Nitrogen fertility effects on Bt δ-endotoxin and nitrogen concentrations of maize during early growth. Agron J. 95:207 Google Scholar
Chen, S, Wu, J, Zhou, B, Huang, J, Zhang, R (2000) On the temporal and spatial expression of Bt toxin protein in Bt transgenic cotton. Acta Gossypii Sinica 12:189–193 Google Scholar
Dong, H, Li, W (2007) Variability of endotoxin expression in Bt transgenic cotton. J Agron Crop Sci. 193:21–29 Google Scholar
Dunlap, JC (1999) Molecular bases for circadian clocks. Cell 96:271–290 Google Scholar
Finnegan, EJ, Llewellyn, DJ, Fitt, GP (1998) What's happening to the expression of the insect protection in field-grown Ingard cotton. Pp 291–297 in Proceedings of the 9th Australian Cotton Conference Broadbeach, Queensland Australian Cotton Growers' Research Association Google Scholar
Greenplate, JT (1999) Quantification of Bacillus thuringiensis insect control protein Cry1Ac over time in Bollgard cotton fruit and terminals. J Econ Entomol 92:1377–1383 Google Scholar
Hanna, SO, Conley, SP, Shaner, GE, Santini, JB (2008) Fungicide application timing and row spacing effect on soybean canopy penetration and grain yield. Agron J. 100:1488–1492 Google Scholar
Kay, SA, Millar, AJ (1995) New models in vogue for circadian clocks. Cell 83:361–364 Google Scholar
Luo, Z, Dong, H, Li, W, Ming, Z, Zhu, Y (2008) Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton. Crop Prot 27:1485–1490 Google Scholar
McClung CR 920060 Plant circadian rhythms. Plant Cell 18:792–803 Google Scholar
Padgette, SR, Kolacz, KH, Delannay, X, Re, DB, LaVallee, BJ, Tinius, CN, Rhodes, WK, Otero, YI, Barry, GF, Eichholtz, DA (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci. 35:1451–1460 Google Scholar
Robinson, AP, Johnson, WG, Simpson, DM (2010) Effect of postemergence applications of 2,4-D on the yield components of DHT soybean. Proceedings North Central Weed Science Society 65:80 Google Scholar
Székács, A, Lauber, É, Juracsek, J, Darvas, B (2010a) Cry1Ab toxin production of MON 810 transgenic maize. Environ Toxicol Chem. 29:182–190 Google Scholar
Székács, A, Lauber, É, Takács, E, Darvas, B (2010b) Detection of Cry1Ab toxin in the leaves of MON 810 transgenic maize. Anal Bioanalyt Chem. 396:2203–2211 Google Scholar
Taylor, NB, Fuchs, RL, MacDonald, J, Shariff, AR, Padgette, SR (1999) Compositional analysis of glyphosate-tolerant soybeans treated with glyphosate. J Agric Food Chem. 47:4469–4473 Google Scholar
Vierstra, RD (1993) Protein degradation in plants. Ann Rev Plant Biol. 44:385–410 Google Scholar
Wright, TR, Shan, G, Walsh, TA, Lira, JM, Cui, C, Song, P, Zhuang, M, Arnold, NL, Lin, G, Yau, K, Russell, SM, Cicchillo, RM, Peterson, MA, Simpson, DM, Zhou, N, Ponsamuel, J, Zhang, Z (2010) Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes. Proc Natl Acad Sci USA 107:20240–20245 Google Scholar