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An Ultrastructural Study of the Effects of 2,4-D on Tobacco Leaves

Published online by Cambridge University Press:  12 June 2017

J. A. White  and
D. D. Hemphill
J. A. White
Affiliation:
Electron Microscope Laboratory, Dep. of Plant Path., Univ. of Mo.—Columbia, Columbia, Missouri, 65201
D. D. Hemphill
Affiliation:
Univ. of Mo.—Columbia, Columbia, Missouri, 65201
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Abstract

Tobacco (Nicotiana tabacum L. ‘Samsun N. N.’) leaves were treated with (2,4-dichlorophenoxy)acetic acid (2,4-D) at a concentration of 500 mg/L by the syringe infiltration technique and then sampled at regular intervals. Electron microscopic examination of the treated tissue revealed that the response of the mesophyll cells to 2,4-D was related to the age of the leaf at the time of treatment. Expanding leaves from the upper portion of the plant exhibited a high degree of tolerance to 2,4-D. Mature, fully-expanded leaves from the lower part of the plant were highly sensitive to the herbicide, with the mesophyll cells undergoing rapid structural breakdown. This breakdown involved a rupturing and disintegration of the tonoplast, plasmalemma, and membranes of chloroplasts and mitochondria.

Type
Research Article
Information
Weed Science , Volume 20 , Issue 5 , September 1972 , pp. 478 - 481
Copyright
Copyright © Weed Science Society of America 

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References

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