Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-22T19:34:35.306Z Has data issue: false hasContentIssue false
  • English
  • Français

An Investigation on the Mechanism of Action of Propachlor

Published online by Cambridge University Press:  12 June 2017

W. B. Duke ,
F. W. Slife ,
J. B. Hanson  and
H. S. Butler
W. B. Duke
Affiliation:
Univ. of Illinois, Urbana, IL 61801 Cornell Univ., Ithaca, NY
F. W. Slife
Affiliation:
Univ. of Illinois, Urbana, IL 61801
J. B. Hanson
Affiliation:
Univ. of Illinois, Urbana, IL 61801
H. S. Butler
Affiliation:
Univ. of Illinois, Urbana, IL 61801
Get access Rights & Permissions [Opens in a new window]

Abstract

Studies were conducted to examine over time the effects of propachlor (2-chloro-N-isopropylacetanilide) on the growth of cucumber (Cucumus sativus L. ‘Straight Eight’) roots and associated biosynthetic reactions. Complete inhibition of root elongation occurred within 16 hr after exposure to propachlor. Inhibition of growth was not found to be a result of an effect on ATP formation or respiration. Protein biosynthesis was reduced several hours before the observed inhibition of growth therefore implicating it as the causal factor. Inhibition of protein synthesis occurred prior to an observed reduction in RNA synthesis suggesting that the primary effect of propachlor is on protein biosynthesis and that its effect on nucleic acid synthesis is secondary. It is concluded that the primary mechanism of action of propachlor is its effect on nascent protein biosynthesis.

Type
Research Article
Information
Weed Science , Volume 23 , Issue 2 , March 1975 , pp. 142 - 147
Copyright
Copyright © 1975 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Cleland, R. 1965. Auxin-induced changes in the presence of actinomycin-D. Plant Physiol. 40:595–600.Google Scholar
2. Colombo, G., Felicetti, L., and Bagolioni, C. 1966. Inhibition of protein synthesis in reticulocytes by antibiotics. 1966. Biochim. Biophys. Acta 119:109–119.Google Scholar
3. Devlin, R.M. and Cunningham, R.P. 1970. The inhibition of gibberellic acid induction of barley endosperms by certain herbicides. Weed Res. 10:316–320.Google Scholar
4. Dhillon, N.S. and LaMar Anderson, J. 1972. Morphological anatomical and biochemical effects of propachlor on seedling growth. Weed Res. 12:182–189.Google Scholar
5. Felicetti, L., Colombo, B., and Bagolioni, C. 1966. Inhibition of protein synthesis in reticulocytes by antibiotics II. Site of action of cycloheximide, streptorutacin A, and pactamycin. Biochim. Biophys. Acta 119:120–129.Google Scholar
6. Goldberg, I.H., Rabenowitz, M., and Reich, E. 1962. Basis of actinomycin action. 1. DNA binding and inhibition of RNA-polymerase synthetic reactions by actinomycin. Proc. Nat. Acad. Sci. 48:2094–2101.Google Scholar
7. Gruenhagen, R.D. and Moreland, D.E. 1971. Effects of herbicides on ATP levels in excised soybean hypocotyls. Weed Sci. 19:319–323.Google Scholar
8. Jaworski, E.G. 1956. Biochemical action of CDAA, a new herbicide. Science 123:847–848.Google Scholar
9. Jaworski, E.G. 1969. Analysis of the mode of action of herbicidal ∝-chloroacetamides. J. Agr. Food Chem. 17:165–171.CrossRefGoogle Scholar
10. Kenefick, D.G. and Hanson, J.B. 1966. The site of olygomycin in corn mitochondria. Biochem. Biophys. Res. Comm. 5:125–129.Google Scholar
11. Key, J.C. 1964. Ribonucleic acid and protein synthesis as essential processes for cell elongation. Plant Physiol. 39:265–370.Google Scholar
12. Leis, J.P. and Keller, E.B. 1970. Protein chain initiating methionine rRNA's in chloroplasts and cytoplasm of wheat leaves. Proc. Nat. Acad. Sci. 67:1593–1599.Google Scholar
13. Lockhart, J.A. 1965. An analysis of irreversible plant cell elongation. J. Theor. Biol. 8:264–275.Google Scholar
14. Lowery, P.H., Rosebrough, N.J., Fair, A.C., and Randall, R.J. 1951. Protein measurements with the folin-phenol reagent. J. Biol. Chem. 193–275.Google Scholar
15. Mann, J.D., Jordan, L.S., and Day, B.E. 1965. A survey of herbicides for their effect on protein synthesis. Plant Physiol. 40:840–843.Google Scholar
16. Mans, R.J. and Novelli, G.D. 1960. A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein. Biochem. and Biophys. Res. Comm. 3:540–543.Google Scholar
17. Moreland, D.E., Malhotra, S.S., Gruenhagen, R.D., and Shokrah, E.H. 1969. Effect of herbicides on RNA and protein synthesis. Weed Sci. 17:556–562.Google Scholar
18. Nathans, D. 1964. Puromycin inhibition of protein synthesis: Incorporation of puromycin into peptide chains. Proc. Natl. Acad. Sci. 51:595–592.Google Scholar
19. Nooden, L.D. and Thimann, K.V. 1966. Action of inhibitors of RNA and protein synthesis on cell enlargement. Plant Physiol. 41:157–164.Google Scholar
20. Wallace, J.M. and Ts'o, P.O.P. 1961. Nucleotide composition of various ribosomes. Biochem. Biophys. Res. Comm. 4:125–129.CrossRefGoogle Scholar
21. Weeks, D.P. and Marcus, A. 1969 Polyribosome isolation in the presence of diethyl pyrocarbonate. Plant Physiol. 44:1291–1294.CrossRefGoogle ScholarPubMed
22. Wettstein, F.O. and Noll, H. 1965. Binding of transfer RNA to ribosome engaged in protein synthesis. Number and properties or ribosomal binding sites. J. Mol. Biol. 11:35–53.Google Scholar
23. Wiskich, J.T. and Bonner, W.D. 1963. Preparation and properties of sweet potatoe mitochondria. Plant Physiol. 38:594–604.Google Scholar