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Acifluorfen Action on Growth and Phenolic Metabolism in Soybean (Glycine max) Seedlings

Published online by Cambridge University Press:  12 June 2017

Robert E. Hoagland
Robert E. Hoagland*
Affiliation:
South. Weed Sci. Lab., U.S. Dep. Agric., Agric. Res. Serv., Stoneville, MS 38776
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Abstract

Three-day-old soybean seedlings were treated with acifluorfen via liquid culture [50 μM in dark, 1 μM in light (200 μE·m–2·s–1)]. Root elongation in the dark was inhibited only slightly (6%) after 96 h. In the light, acifluorfen inhibited root elongation after 48 h; after 96 h, inhibition was 32%. Hypocotyl length was not affected in either the light or dark. Soluble hydroxyphenolic content per axis was unaffected in the dark but was reduced by about 13 to 25% at 24 to 96 h in the light. Extractable phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity per axis was unaltered by herbicide in the dark. A brief transient increase in PAL activity (4 to 24 h) in axes of light-grown seedlings in the presence of acifluorfen was followed by reduced enzyme levels compared to light-grown controls at 72 and 96 h. Although light is required for maximal herbicide action, there was no effect on anthocyanin or chlorophyll accumulation or on the chlorophyll a/b ratio in hypocotyls or light-grown seedlings.

Keywords

Acifluorfen, 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acidsoybean, Glycine max (L.) Merr. ‘Hill’Acifluorfendiphenyl ether herbicidesenzymeherbicide actionphenylalanine ammonialyase [EC 4.3.1.5]phenylpropanoid metabolismsoybean
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 37 , Issue 6 , November 1989 , pp. 743 - 747
Copyright
Copyright © 1989 by the Weed Science Society of America 

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References

Literature Cited

1. Amrhein, N. and Zenk, M. H. 1971. Untersuchungen gur rolle der phenylalanine-ammonium-lyase (PAL) bie der regulation der flavonoidsynthese in buchweigen (Fagopyrum esculentum Moench.). Z. Pflanzenphysiol. 64:145–168.Google Scholar
2. Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris . Plant Physiol. 24:–15.CrossRefGoogle Scholar
3. Ashton, F. M. and Crafts, A. S. 1981. Mode of Action of Herbicides. John Wiley and Sons, New York. 525 pp.Google Scholar
4. Betz, B., Schafer, E., and Hahlbrock, K. 1978. Light-induced phenylalanine ammonia-lyase in cell suspension cultures of Petroselinum hortense. Quantitative comparison rates of synthesis and degradation. Arch. Biochem. Biophys. 190:126–135.CrossRefGoogle ScholarPubMed
5. Camm, E. L. and Towers, G.H.N. 1977. Phenylalanine ammonia-lyase. Pages 169–188 in Reinhold, L., Harborne, J. B., and Swain, T., eds. Progress in Phytochemistry. Vol. 4. Pergamon Press, Oxford.Google Scholar
6. Cosio, E. G., Weissenboeck, G., and McClure, J. W. 1985. Acifluorfen-induced isoflavonoids and enzymes of their biosynthesis in mature soybean leaves. Plant Physiol. 78:14–19.Google Scholar
7. Duke, S. O. 1985. Biosynthesis of phenolic compounds; chemical manipulation in higher plants. Pages 113–131 in Thompson, A. C., ed. The Chemistry of Allelopathy. ACS Symp. Ser. No. 268. Am. Chem. Soc., Washington, DC.Google Scholar
8. Duke, S. O. and Hoagland, R. E. 1978. Effects of glyphosate on metabolism of phenolic compounds. I. Induction of phenylalanine ammonia-lyase activity in dark-grown maize roots. Plant Sci. Lett. 11: 185–190.CrossRefGoogle Scholar
9. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1979. Effects of glyphosate on metabolism of phenolic compounds. IV. Phenylalanine ammonia-lyase activity, free amino acids, and soluble hydroxyphenolic compounds in axes of light-grown soybeans. Physiol. Plant. 46: 307–317.Google Scholar
10. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1980. Effects of glyphosate on metabolism of phenolic compounds. V. L-α-aminooxy-β-phenylpropionic acid and glyphosate effects on phenylalanine ammonia-lyase in soybean seedlings. Plant Physiol. 65:17–21.Google Scholar
11. Duke, S. O. and Naylor, A. W. 1974. Effects of light on phenylalanine ammonia-lyase activity in dark-grown Zea mays (L.) seedlings. Plant Sci. Lett. 2:289–293.Google Scholar
12. Frear, D. S., Swanson, H. R., and Mansager, E. R. 1983. Acifluorfen metabolism in soybean: diphenylether bond cleavages and the formation of homoglutathione, cysteine and glucose conjugates. Pestic. Biochem. Physiol. 20:299–310.CrossRefGoogle Scholar
13. Gaba, V., Cohen, N., Shalltiel, Y., Bene-Amotz, A., and Gressel, J. 1988. Light-requiring acifluorfen action in the absence of bulk photosynthetic pigments. Pestic. Biochem. Biophys. 31:1–12.Google Scholar
14. Gillham, D. J. and Dodge, A. D. 1987. Studies into the action of the diphenyl ether herbicides. Part II. The interaction with photosynthetic electron transport reactions. Pestic. Sci. 19:25–34.Google Scholar
15. Havir, E. A. and Hanson, K. R. 1970. L-Phenylalanine ammonia-lyase (potato tubers). Pages 575–581 in Tabor, H. and Tabor, C. W., eds. Methods in Enzymology. Vol. 17A. Academic Press, New York.Google Scholar
16. Hoagland, R. E. 1980. Effects of glyphosate on metabolism of phenolic compounds: VI. Effects of glyphosate and glyphosate metabolites on phenylalanine ammonia-lyase activity, growth, and protein, chlorophyll, and anthocyanin levels in soybean (Glycine max) seedlings. Weed Sci. 28:393–400.CrossRefGoogle Scholar
17. Hoagland, R. E. 1981. Acifluorfen action on growth and phenylpropanoid metabolism in axes of soybean (Glycine max) seedlings. Plant Physiol. Suppl. 67:112.Google Scholar
18. Hoagland, R. E. and Duke, S. O. 1981. Effects of herbicides on growth and extractable phenylalanine ammonia-lyase activity in light- and dark-grown soybean (Glycine max) seedlings. Weed Sci. 29:433–439.Google Scholar
19. Hoagland, R. E. and Duke, S. O. 1983. Relationships between phenylalanine ammonia-lyase activity and physiological responses of soybean (Glycine max) seedlings to herbicides. Weed Sci. 31:845–852.Google Scholar
20. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1978. Effects of glyphosate on metabolism of phenolic compounds. II. Influence on soluble hydroxyphenolic compound, free amino acid and soluble protein levels in dark-grown maize roots. Plant Sci. Lett. 13:291–299.CrossRefGoogle Scholar
21. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1979. The effect of glyphosate on metabolism of phenolic compounds. III. Phenylalanine ammonia-lyase activity, free amino acids, soluble protein, and hydroxyphenolic compounds in axes of dark-grown soybeans. Physiol. Plant. 46:357–366.Google Scholar
22. Hoagland, R. E., Hunter, D. J., and Salin, M. L. 1986. Acifluorfen and oxidized ubiquinone in soybean mitochondria. Plant Cell Physiol. 27: 11–15.Google Scholar
23. Hyodo, H., Kuroda, H., and Yang, S. F. 1978. Induction of phenylalanine ammonia-lyase and increases in phenolics in lettuce leaves in relation to the development of russet spotting caused by ethylene. Plant Physiol. 62:31–35.Google Scholar
24. Jangaard, N. O. 1974. The effect of herbicides, plant growth regulators and other compounds on phenylalanine and ammonia-lyase activity. Phytochemistry 13:1769–1775.Google Scholar
25. Johnson, W. O., Kollman, G. T., Swithenbank, C., and Yih, R. H. 1978. RH-6201 (Blazer): A new broad spectrum herbicide for postemergence use in soybeans. J. Agric. Food Chem. 26:285–286.CrossRefGoogle Scholar
26. Kömives, T. and Casida, J. E. 1982. Diphenylether herbicides: Effects of acifluorfen on phenylpropanoid biosynthesis and phenylalanine ammonia-lyase activity in spinach. Pestic. Biochem. Physiol. 18: 191–196.Google Scholar
27. Kömives, T. and Casida, J. E. 1983. Acifluorfen increases the leaf content of phytoalexins and stress metabolites in several crops. J. Agric. Food Chem. 31:751–755.Google Scholar
28. Matringe, M. and Scalla, R. 1988. Studies on the mode of action of acifluorfen-methyl in nonchlorophyllous soybean cells. Plant Physiol. 86:619–622.Google Scholar
29. Matsunaka, S. 1976. Diphenyl ethers. Pages 709–739 in Kearney, P. C. & Kaufman, D. D., eds. Herbicides-Chemistry, Degradation and Mode of Action. Vol. 2. Marcel-Dekker, New York.Google Scholar
30. Murphy, J. B. and Kies, M. W. 1960. Note on a spectrophotometric determination of proteins in dilute solutions. Biochim. Biophys. Acta 45:382–384.CrossRefGoogle Scholar
31. Nurit, F., Ravanel, P., and Tissut, M. 1988. The photodependent effort of LS 82556 and acifluorfen in cucumber cotyledon pieces: The possible indirect involvement of photosynthesis. Pestic. Biochem. Biophys. 31:67–73.Google Scholar
32. Orr, G. L. and Hess, F. D. 1982. Proposed site(s) of action of new diphenylether herbicides. Pages 131–152 in Moreland, D. E., St. John, J. B., and Hess, F. D., eds. Biochemical Responses Induced by Herbicides. ACS Symp. Ser. No. 181. Am. Chem. Soc., Washington, DC.Google Scholar
33. Osman, S. F. and Fett, W. F. 1983. Isoflavone glucoside stress metabolites of soybean leaves. Phytochemistry 22:1921–1923.Google Scholar
34. Singleton, Y. L. and Rossi, J. A. Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotunstic acid reagents. Am. J. Enol. Vitic. 16:144–158.Google Scholar
35. Smith, D. A. 1982. Toxicity of phytoalexins. Pages 218–252 in Bailey, J. A. and Mansfield, J. W., eds. Phytoalexins. John Wiley and Sons, New York.Google Scholar
36. Smith, H., Billett, E. E., and Giles, A. B. 1977. Photocontrol of gene expression in higher plants. Pages 93–127 in Smith, H., ed. Regulation of Enzyme Synthesis and Activity in Higher Plants. Academic Press, New York.Google Scholar
37. Suzuki, T., Holden, I., and Casida, J. E. 1981. Diphenyl ether herbicides remarkably elevate the content in Spinacia oleracea of (E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxy-3-meth-oxy-phenyl)ethyl]-2-propenamide. J. Agric. Food Chem. 29:992–995.Google Scholar
38. Weed Science Society of America. 1983. Herbicide Handbook. 5th ed. Champaign, IL.Google Scholar
39. Zucker, M. 1972. Light and enzymes. Annu. Rev. Plant Physiol. 23: 133–156.CrossRefGoogle Scholar