Removing the epicuticular wax from mature pads (cladophylls) of Lindheimer pricklypear increased picloram absorption by four- to sixfold in the laboratory, while the addition of surfactant had little effect on absorption. Absorption decreased with increasing pH of the picloram solution, indicating that picloram diffused through the cuticle as the undissociated molecule. Picloram entered detached pads at the areoles more readily than through the surrounding cuticle. In the glasshouse, whole plants consisting of an old, mature pad supporting a young, growing pad absorbed picloram very slowly whether picloam was applied as a spray to old or young pads or to the soil. About 90 and 80% of the applied picloram remained on the waxy surface of old and new pads, respectively, and about 2% of the applied picloram was recovered from within the epicuticular wax after 30 days. Picloram concentrations within pads treated in the glasshouse were greater when the herbicide was applied to new pads (4.6 μg/g) than old pads (1.9 μg/g) after 30 days. More picloram was translocated basipetally from treated new pads to untreated old pads than in the opposite direction, but concentrations in untreated pads were low (<1 μg/g). Little picloram was absorbed by roots, compared to pads, and little was translocated into or out of roots. These results conflict with the view that the effectiveness of picloram for pricklypear control is attributable to extensive root uptake and acropetal transport. However, observations of plants 6 months after treatment indicated that soil applications were more effective than sprays in the glasshouse.