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Use of PCR-based molecular markers to identify weedy Amaranthus species

Published online by Cambridge University Press:  12 June 2017

Denise K. Wetzel ,
Michael J. Horak  and
Daniel Z. Skinner
Denise K. Wetzel
Affiliation:
Department of Agronomy, Kansas State University, Manhattan, KS 66506
Daniel Z. Skinner
Affiliation:
USDA-ARS, Department of Agronomy, Kansas State University, Manhattan, KS 66506
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Abstract

Weedy species of the genus Amaranthus, commonly referred to as pigweeds, have increased in frequency and severity over the past few years. Identification of these weeds is difficult because of similar morphological characteristics among species and variation within species. Studies were initiated to develop a molecular marker identification system utilizing restriction enzyme analysis of amplified ribosomal DNA (rDNA). A set of polymerase chain reaction (PCR) markers was developed to distinguish 10 weedy species of pigweeds. Restriction-site variation, utilizing five endonucleases, within the internal transcribed spacers (ITS) of the rDNA allowed for the positive identification of eight species and one pair of species. These markers will be useful for biological and ecological studies on the genus.

Keywords

Amaranthus spp. AMASSITS1 and ITS2Amaranthus albus L. AMAAL, tumble pigweedAmaranthus arenicola I.M. Johnst. AMAAR, sandhills amaranthAmaranthus blitoides S. Wats. AMABL, prostrate pigweedAmaranthus hybridus L. AMACH, smooth pigweedAmaranthus palmeri S. Wats. AMAPA, Palmer amaranthAmaranthus powellii S. Wats. AMAPO, Powell amaranthAmaranthus retroflexus L. AMARE, redroot pigweedAmaranthus rudis Sauer AMATA, common waterhempAmaranthus spinosus L. AMASPspiny amaranthAmaranthus tuberculatus (Moq.) J.D. Sauer AMATUtall waterhemp
Type
Weed Biology and Ecology
Information
Weed Science , Volume 47 , Issue 5 , October 1999 , pp. 518 - 523
Copyright
Copyright © 1999 by the Weed Science Society of America 

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References

Literature Cited

Chan, K. F. and Sun, M. 1997. Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of Amaranthus . Theor. Appl. Genet. 95:865–873.CrossRefGoogle Scholar
Dellaporta, S., Woods, J., and Hicks, J. 1983. A plant DNA minipreparation, version II. Plant Mol. Biol. Rep. 1:19–21.CrossRefGoogle Scholar
Great Plains Flora Association. 1986. Flora of the Great Plains. Lawrence, KS: University Press of Kansas, pp. 179–184.Google Scholar
Hauptli, H. and Jain, S. 1984. Allozyme variation and evolutionary relationships of grain amaranths (Amaranthus spp.). Theor. Appl. Genet. 69:153–169.CrossRefGoogle ScholarPubMed
Horak, M. J., Peterson, D. E., Chessman, D. J., and Wax, L. M. 1994. Pigweed Identification: A Pictoral Guide to the Common Pigweeds of the Great Plains. Manhattan, KS: Kansas State University. 12 p.Google Scholar
Jorgensen, R. A. and Cluster, P. D. 1988. Modes and tempos in the evolution of nuclear ribosomal DNA: new characters for evolutionary studies and new markers for genetic and population studies. Ann. Mo. Bot. Gard. 75:1238–1247.CrossRefGoogle Scholar
Kirkpatrick, B. A. 1995. Interspecific Relationships Within the Genus Amaranthus (Amaranthaceae). Ph.D. dissertation. Texas A&M University, College Station, TX. 87 p.Google Scholar
Klingman, T. E. and Oliver, L. R. 1994. Palmer amaranth (Amaranthus palmeri) interference in soybeans (Glycine max). Weed Sci. 42:568–573.Google Scholar
Knezevic, S.Z., Horak, M. J., and Vanderlip, R. L. 1997. Relative time of redroot pigweed (Amaranthus retroflexus L.) emergence is critical in pigweed–sorghum [Sorghum bicolor (L.) Moench] competition. Weed Sci. 45:502–508.CrossRefGoogle Scholar
Lanoue, K. Z., Wolf, P. G., Browning, S., and Hood, E. E. 1996. Phylogenetic analysis of restriction-site variation in wild and cultivated Amaranthus species (Amaranthaceae). Theor. Appl. Genet. 93:722–732.CrossRefGoogle ScholarPubMed
Mayo, C. M., Horak, M. J., Peterson, D. E., and Boyer, J. E. 1995. Differential control of four Amaranthus species by six postemergence herbicides in soybean (Glycine max). Weed Technol. 9:141–147.CrossRefGoogle Scholar
Murphy, S. D., Yankubu, Y., Weise, S. F., and Swanton, C. J. 1996. Effect of planting patterns and inter-row cultivation on competition between corn (Zea mays) and late emerging weeds. Weed Sci. 44:856–870.CrossRefGoogle Scholar
Murray, M. J. 1940. The genetics and sex determination in the family Amaranthaceae. Genetics 25:409–431.CrossRefGoogle ScholarPubMed
Sauer, J. D. 1955. Revision of the dioecious amaranths. Madrono 13:5–46.Google Scholar
Sauer, J. D. 1967. The grain amaranths and their relatives: a revised taxonomic and geographic survey. Ann. Mo. Bot. Gard. 54:103–137.CrossRefGoogle Scholar
Scott, L. J., Lange, C. L., Graham, G. C., and Yeates, D. K. 1998. Genetic diversity and origin of siam weed (Chromolaena odorata) in Australia. Weed Technol. 12:27–31.CrossRefGoogle Scholar
Sweat, J. K., Horak, M. J., Peterson, D. E., Lloyd, R. W., and Boyer, J. E. 1998. Herbicide efficacy on four Amaranthus species in soybean (Glycine max). Weed Technol. 12:315–321.CrossRefGoogle Scholar
Transue, D. K., Fairbanks, D. J., Robison, L. R., and Andersen, W. R. 1994. Species identification by RAPD analysis of grain amaranth genetic resources. Crop Sci. 34:1385–1389.CrossRefGoogle Scholar
Wax, L. M. 1995. Pigweeds of the Midwest—distribution, importance and management. Proc. Iowa Integrated Crop Manag. Conf. 7:239–242.Google Scholar
Webster, T. M. and Coble, H. D. 1997. Changes in the weed species composition of the Southern United States: 1974 to 1995. Weed Technol. 11:308–317.CrossRefGoogle Scholar
White, T. J., Brims, T., Lee, S., and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315–322 in PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press.Google Scholar
Williams, J.G.K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535.CrossRefGoogle ScholarPubMed