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Use of Alcaligenes eutrophus as a Source of Genes for 2,4-D Resistance in Plants

Published online by Cambridge University Press:  12 June 2017

E. J. Perkins ,
C. M. Stiff  and
P. F. Lurquin
E. J. Perkins
Affiliation:
Program in Genetics and Cell Biology, Washington State Univ., Pullman, WA 99164-4350 U.S.A.
C. M. Stiff
Affiliation:
Program in Genetics and Cell Biology, Washington State Univ., Pullman, WA 99164-4350 U.S.A.
P. F. Lurquin
Affiliation:
Program in Genetics and Cell Biology, Washington State Univ., Pullman, WA 99164-4350 U.S.A.
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Abstract

The soil bacterium Alcaligenes eutrophus is able to degrade phenoxy herbicides such as 2,4-D [(2,4-dichlorophenoxy)acetic acid]. It has been shown that several degradative genes are located on a large plasmid harbored by this organism. We have demonstrated by HPLC and gas chromatography that 2,4-dichlorophenol is a major metabolite resulting from 2,4-D degradation by A. eutrophus. A portion of the large plasmid carrying the 2,4-D catabolic genes has been cloned and mapped by restriction endonuclease digestion. Overlapping subclones have been produced and were used in conjugation experiments in order to identify the first gene involved in 2,4-D detoxification. Once sequenced, this gene will be transferred to dicotyledonous plant cells using available vector systems.

Keywords

2,4-D monooxygenase geneplant cell transformationgas chromatography2,4-dichlorophenol toxicity
Type
Research Article
Information
Weed Science , Volume 35 , Issue S1: Genetic Engineering for Herbicide Resistance , 1987 , pp. 12 - 18
Copyright
Copyright © 1987 by the Weed Science Society of America 

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