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Two-tier witchweed (Striga hermonthica) resistance in wild pearl millet (Pennisetum glaucum) 29Aw

Published online by Cambridge University Press:  15 February 2021

Olivier Dayou Open the ORCID record for Olivier Dayou [Opens in a new window] ,
Willy Kibet Open the ORCID record for Willy Kibet [Opens in a new window] ,
Patroba Ojola Open the ORCID record for Patroba Ojola [Opens in a new window] ,
Prakash Irappa Gangashetty Open the ORCID record for Prakash Irappa Gangashetty [Opens in a new window] ,
Susann Wicke Open the ORCID record for Susann Wicke [Opens in a new window]  and
Steven Runo Open the ORCID record for Steven Runo [Opens in a new window]
Olivier Dayou
Affiliation:
Master’s Student, Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, Nairobi, Kenya
Willy Kibet
Affiliation:
Research Associate, Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, Nairobi, Kenya
Patroba Ojola
Affiliation:
Lecturer, Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, Nairobi, Kenya
Prakash Irappa Gangashetty
Affiliation:
Scientist, Pearl Millet Breeding International Crops Research Institute for the Semi-Arid Tropics, Niamey, Niger
Susann Wicke
Affiliation:
Associate Professor, Plant Evolutionary Biology, Institute for Evolution and Biodiversity, University of Münster, Münster, Germany; current: Full Professor, Evolutionary Botany and Biodiversity, Institute for Biology, Humboldt University Berlin, Berlin, Germany
Steven Runo*
Affiliation:
Associate Professor, Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, Nairobi, Kenya
*
Author for correspondence: Steven Runo, Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, P.O. Box 43844, 00100 GPO, Nairobi, Kenya. Email: [email protected]
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Abstract

The parasitic plant purple witchweed [Striga hermonthica (Delile) Benth.] poses a serious threat to cereal production in sub-Saharan Africa. Under natural infestation, the wild pearl millet [Pennisetum glaucum (L.) R. Br.] line 29Aw demonstrates resistance against the parasite, but the mechanism of its resistance is unknown. Striga resistance can be due to: (1) low induction of Striga germination (pre-attachment resistance) or (2) inhibition of parasite attachment and development (post-attachment resistance). Germination bioassays and root chamber (rhizotron) resistance-screening assays were used to determine the extent of pre- and post-attachment Striga resistance in 29Aw compared with the Striga-susceptible ‘SOSAT-C88-P10’ variety. Regarding pre-attachment resistance, 29Aw stimulated 10-fold less Striga seed germination at a maximum germination distance of 7.96 ± 2.75 mm from the host root compared with 35.94 ± 2.88 mm in SOSAT-C88-P10. Post-attachment resistance revealed 10- to 19-fold fewer and 2.5-fold shorter Striga seedlings with 28-fold less biomass growing on 29Aw compared with SOSAT-C88-P10. Microscopic analysis showed that Striga penetration in 29Aw was blocked at the endodermal and cortical levels. Post-attachment resistance in 29Aw was further supported by fewer (22%) Striga–host vascular connections in 29Aw compared with 79% in SOSAT-C88-P10. Together, these findings demonstrate that 29Aw harbors both pre- and post-attachment resistance mechanisms against S. hermonthica.

Keywords

Striga post-attachment resistanceStriga pre-attachment resistance
Type
Research Article
Information
Weed Science , Volume 69 , Issue 3 , May 2021 , pp. 300 - 306
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Copyright
© The Author(s), 2021. Published by Cambridge University Press on behalf of the Weed Science Society of America

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Footnotes

Associate Editor: Bhagirath Chauhan, The University of Queensland

References

Amusan, IO, Rich, PJ, Menkir, A, Housley, T, Ejeta, G (2008) Resistance to Striga hermonthica in a maize inbred line derived from Zea diploperennis. New Phytol 178:157–166 CrossRefGoogle Scholar
Arnaud, MC, Véronési, C, Thalouarn, P (1999) Physiology and histology of resistance to Striga hermonthica in Sorghum bicolor var. Framida. Functional Plant Biol 26:63–70 CrossRefGoogle Scholar
Atera, EA, Itoh, K, Onyango, JC (2011) Evaluation of ecologies and severity of Striga weed on rice in sub-Saharan Africa. Agric Biology J North Am 2:753–760 CrossRefGoogle Scholar
Awad, AA, Sato, D, Kusumoto, D, Kamioka, H, Takeuchi, Y, Yoneyama, K (2006) Characterization of strigolactones, germination stimulants for the root parasitic plants Striga and Orobanche, produced by maize, millet and sorghum. Plant Growth Regul 48:221–227 Google Scholar
Badu-Apraku, B, Adewale, S, Paterne, A, Gedil, M, Asiedu, R (2020) Identification of QTLs controlling resistance/tolerance to Striga hermonthica in an extra-early maturing yellow maize population. Agronomy 10:1168 CrossRefGoogle Scholar
Bellis, ES, Kelly, EA, Lorts, CM, Gao, H, DeLeo, VL, Rouhan, G, Budden, A, Bhaskara, GB, Hu, Z, Muscarella, R, Timko, MP, Nebie, B, Runo, SM, Chilcoat, ND, Juenger, TE, et al. (2020) Genomics of sorghum local adaptation to a parasitic plant. Proc Natl Acad Sci USA 117:4243–4251 CrossRefGoogle ScholarPubMed
Cameron, DD, Coats, AM, Seel, WE (2006) Differential resistance among host and non-host species underlies the variable success of the hemi-parasitic plant Rhinanthus minor . Ann Bot 98:1289–1299 CrossRefGoogle ScholarPubMed
Cissoko, M, Boisnard, A, Rodenburg, J, Press, MC, Scholes, JD (2011) New Rice for Africa (NERICA) cultivars exhibit different levels of post-attachment resistance against the parasitic weeds Striga hermonthica and Striga asiatica. New Phytol 192:952–963 CrossRefGoogle Scholar
Dörr, I (1997) How Striga parasitizes its host: a TEM and SEM study. Ann Bot 79:463–472 CrossRefGoogle Scholar
Echevarria-Zomeno, S, Perez-de-Luque, A, Jorrin, J, Maldonado, AM (2006) Pre-haustorial resistance to broomrape (Orobanche cumana) in sunflower (Helianthus annuus): cytochemical studies. J Exp Bot 57:4189–4200 CrossRefGoogle ScholarPubMed
Ejeta, G (2007) The Striga scourge in Africa: a growing pandemic. Pages 3–16 in Ejeta, G, Gressel, J, eds. Integrating New Technologies for Striga Control. Singapore: World Scientific CrossRefGoogle Scholar
Gobena, D, Shimels, M, Rich, PJ, Ruyter-Spira, C, Bouwmeester, H, Kanuganti, S, Mengiste, T, Ejeta, G (2017) Mutation in sorghum LOW GERMINATION STIMULANT 1 alters strigolactones and causes Striga resistance. Proc National Acad Sci USA 114:4471–4476 CrossRefGoogle ScholarPubMed
Goldwasser, Y, Hershenhorn, J, Plakhine, D, Kleifeld, Y, Rubin, B (1999) Biochemical factors involved in vetch resistance to Orobanche aegyptiaca . Physiol Mol Plant Pathol 54:87–96 CrossRefGoogle Scholar
Gurney, AL, Grimanelli, D, Kanampiu, F, Hoisington, D, Scholes, JD, Press, MC (2003) Novel sources of resistance to Striga hermonthica in Tripsacum dactyloides, a wild relative of maize. New Phytol 160:557–568 CrossRefGoogle ScholarPubMed
Gurney, AL, Slate, J, Press, MC, Scholes, JD (2006) A novel form of resistance in rice to the angiosperm parasite Striga hermonthica . New Phytol 169:199–208 CrossRefGoogle ScholarPubMed
Haussmann, BIG, Hess, DE, Omanya, GO, Folkertsma, RT, Reddy, BVS, Kayentao, M, Welz, HG, Geiger, HH (2004) Genomic regions influencing resistance to the parasitic weed Striga hermonthica in two recombinant inbred populations of sorghum. Theor Appl Genet 109:1005–1016 CrossRefGoogle ScholarPubMed
Hess, DE, Ejeta, G, Butler, LG (1992) Selecting sorghum genotypes expressing a quantitative biosynthetic trait that confers resistance to Striga . Phytochemistry 31:493–497 CrossRefGoogle Scholar
Hudson, JP (1967) Sand and Water Culture Methods Used in the Study of Plant Nutrition. East Malling, Maidstone, Kent, UK: Experimental Agriculture. 547 pGoogle Scholar
[ICRISAT] International Crops Research Institute for the Semi-Arid Tropics (2021) Pearl Millet: Facts and Figures. http://exploreit.icrisat.org/profile/Pearl%20Millet/178. Accessed: March 6, 2021Google Scholar
Izge, AU, Song, IM (2013). Pearl millet breeding and production in Nigeria: problems and prospects. Journal of Environmental Issues and Agriculture in Developing Countries 5:25 Google Scholar
Jamil, M, Rodenburg, J, Charnikhova, T, Bouwmeester, HJ (2011) Pre-attachment Striga hermonthica resistance of New Rice for Africa (NERICA) cultivars based on low strigolactone production. New Phytol 192:964–975 CrossRefGoogle Scholar
Jukanti, AK, Gowda, CLL, Rai, KN, Manga, VK, Bhatt, RK (2016) Crops that feed the world 11. Pearl millet (Pennisetum glaucum L.): an important source of food security, nutrition and health in the arid and semi-arid tropics. Food Secur 8:307–329 CrossRefGoogle Scholar
Kanampiu, F, Makumbi, D, Mageto, E, Omanya, G, Waruingi, S, Musyoka, P, Ransom, J (2018) Assessment of management options on Striga infestation and maize grain yield in Kenya. Weed Sci 66:516–524 CrossRefGoogle ScholarPubMed
Kountche, BA, Hash, CT, Dodo, H, Laoualy, O, Sanogo, MD, Timbeli, A, Vigouroux, Y, This, D, Nijkamp, R, Haussmann, BIG (2013) Development of a pearl millet Striga-resistant genepool: response to five cycles of recurrent selection under Striga-infested field conditions in West Africa. Field Crop Res 154:82–90 CrossRefGoogle Scholar
Li, J, Timko, MP (2009) Gene-for-gene resistance in Striga-cowpea associations. Science 325:1094–1094 CrossRefGoogle ScholarPubMed
Lopez, L, Bellis, ES, Wafula, E, Hearne, SJ, Honaas, L, Ralph, PE, Timko, MP, Unachukwu, N, dePamphilis, CW, Lasky, JR (2019) Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica). Weed Sci 67:397–411 CrossRefGoogle Scholar
Matusova, R, Rani, K, Verstappen, FWA, Franssen, MCR, Beale, MH, Bouwmeester, HJ (2005) The strigolactone germination stimulants of the plant-parasitic Striga and Orobanche spp. are derived from the carotenoid pathway. Plant Physiol 139:920–934 CrossRefGoogle ScholarPubMed
Mbuvi, DA, Masiga, CW, Kuria, E, Masanga, J, Wamalwa, M, Mohamed, A, Odeny, DA, Hamza, N, Timko, MP, Runo, S (2017) Novel sources of witchweed (Striga) resistance from wild sorghum accessions. Front Plant Sci 8:271 CrossRefGoogle ScholarPubMed
Mohamed, A, Ellicott, A, Housley, TL, Ejeta, G (2003) Hypersensitive response to infection in sorghum. Crop Sci 43:1320–1324 CrossRefGoogle Scholar
Musselman, LJ, Hepper, FN (1986) The witchweeds (Striga, Scrophulariaceae) of the Sudan Republic. Kew Bull 41:205 CrossRefGoogle Scholar
Mutinda, SM, Masanga, J, Mutuku, JM, Runo, S, Alakonya, A (2018) KSTP 94, an open-pollinated maize variety has post attachment resistance to purple witchweed (Striga hermonthica). Weed Sci 66:525–529 CrossRefGoogle Scholar
Omanya, GO, Weltzien-rattunde, E, Sogodogo, D, Sanogo, M, Hanssens, N, Guero, Y, Zangre, R (2007) Participatory varietal selection with improved pearl millet in West Africa. Exp Agric 43:5–19 CrossRefGoogle Scholar
Parker, C (2009) Observations on the current status of Orobanche and Striga problems worldwide. Pest Manag Sci 65:453–459 CrossRefGoogle ScholarPubMed
Runo, S, Kuria, EK (2018) Habits of a highly successful cereal killer, Striga . PLoS Pathog 14:e1006731–e1006736 CrossRefGoogle ScholarPubMed
Swarbrick, PJ, Scholes, JD, Press, MC, Slate, J (2009) A major QTL for resistance of rice to the parasitic plant Striga hermonthica is not dependent on genetic background. Pest Manag Sci 65:528–532 Google Scholar
Wilson, JP, Hess, DE, Hanna, WW (2007) Resistance to Striga hermonthica in wild accessions of the primary gene pool of Pennisetum glaucum. Phytopathology 90:1169–1172 CrossRefGoogle Scholar
Wilson, JP, Hess, DE, Hanna, WW, Kumar, KA, Gupta, SC (2004) Pennisetum glaucum subsp. monodii accessions with Striga resistance in West Africa. Crop Prot 23:865–870 CrossRefGoogle Scholar
Yoneyama, K, Arakawa, R, Ishimoto, K, Kim, HI, Kisugi, T, Xie, X, Nomura, T, Kanampiu, F, Yokota, T, Ezawa, T, Yoneyama, K (2015) Difference in Striga-susceptibility is reflected in strigolactone secretion profile, but not in compatibility and host preference in arbuscular mycorrhizal symbiosis in two maize cultivars. New Phytol 206:983–989 CrossRefGoogle Scholar
Yoshida, S, Shirasu, K (2009) Multiple layers of incompatibility to the parasitic witchweed, Striga hermonthica . New Phytol 183:180–189 CrossRefGoogle ScholarPubMed