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Transfer and Expression of ALS Inhibitor Resistance from Palmer Amaranth (Amaranthus palmeri) to an A. spinosus × A. palmeri Hybrid

Published online by Cambridge University Press:  20 January 2017

William T. Molin ,
Vijay K. Nandula ,
Alice A. Wright  and
Jason A. Bond
William T. Molin*
Affiliation:
Crop Production Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776
Vijay K. Nandula
Affiliation:
Crop Production Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776
Alice A. Wright
Affiliation:
Crop Production Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776
Jason A. Bond
Affiliation:
Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776
*
Corresponding author's E-mail: [email protected]
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Abstract

Transfer of herbicide resistance among closely related weed species is a topic of growing concern. A spiny amaranth × Palmer amaranth hybrid was confirmed resistant to several acetolactate synthase (ALS) inhibitors including imazethapyr, nicosulfuron, pyrithiobac, and trifloxysulfuron. Enzyme assays indicated that the ALS enzyme was insensitive to pyrithiobac and sequencing revealed the presence of a known resistance conferring point mutation, Trp574Leu. Alignment of the ALS gene for Palmer amaranth, spiny amaranth, and putative hybrids revealed the presence of Palmer amaranth ALS sequence in the hybrids rather than spiny amaranth ALS sequences. In addition, sequence upstream of the ALS in the hybrids matched Palmer amaranth and not spiny amaranth. The potential for transfer of ALS inhibitor resistance by hybridization has been demonstrated in the greenhouse and in field experiments. This is the first report of gene transfer for ALS inhibitor resistance documented to occur in the field without artificial/human intervention. These results highlight the need to control related species in both field and surrounding noncrop areas to avoid interspecific transfer of resistance genes.

Keywords

ImazethapyrnicosulfuronpyrithiobactrifloxysulfuronPalmer amaranth Amaranthus palmeri S. Watsspiny amaranth (Amaranthus spinosus L.)Acetolactate synthaseALS enzymecross resistancehybridmutation
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 64 , Issue 2 , June 2016 , pp. 240 - 247
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Associate editor for this paper: Chris Preston, University of Adelaide.

References

Literature Cited

Franssen, AS, Skinner, DZ, Al-Khatib, K, Horak, MJ, Kulakow, PA (2001) Interspecific hybridization and gene flow of ALS resistance in Amaranthus species. Weed Sci 49:598–606 Google Scholar
Gaines, TA, Ward, SM, Bukun, B, Preston, C, Leach, JE, Westra, P (2011) Interspecific hybridization transfers a previously unknown glyphosate resistance mechanism in Amaranthus species. Evol Appl 5:29–38 Google Scholar
Gaines, TA, Zhang, W, Wang, D, Bukun, B, Chisholm, ST, Shaner, DL, Nissen, SJ, Patzoldt, WL, Tranel, PJ, Culpepper, AS, Grey, TL, Webster, TM, Vencill, WK, Sammons, RD, Jiang, J, Preston, C, Leach, JE, Westra, P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci USA 107:1029–1034 Google Scholar
Heap, IM (2015) International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed August 24, 2015Google Scholar
Kearse, M, Moir, R, Wilson, A, Stones-Havas, S, Cheung, M, Sturrock, S, Buxton, S, Cooper, A, Markowitz, S, Duran, C, Thierer, T, Ashton, B, Mentjies, P, Drummond, A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649 Google Scholar
Mississippi State University (2015) Weed Control Guidelines for Mississippi. Mississippi State, MS: Mississippi State University. 240 pGoogle Scholar
Nandula, VK, Messersmith, CG (2000) Mechanism of wild oat (Avena fatua L.) resistance to imazamethabenz-methyl. Pestic Biochem Physiol 68:148–155 Google Scholar
Nandula, VK, Reddy, KN, Koger, CH, Poston, DH, Rimando, AM, Duke, SO, Bond, JA, Ribeiro, DN (2012) Multiple resistance to glyphosate and pyrithiobac in Palmer amaranth (Amaranthus palmeri) from Mississippi and response to flumiclorac. Weed Sci 60:179–188 Google Scholar
Nandula, VK, Wright, AA, Bond, JA, Ray, JD, Eubank, TW, Molin, WT (2014) EPSPS amplification in glyphosate-resistant spiny amaranth (Amaranthus spinosus): a case of gene transfer via interspecific hybridization from glyphosate-resistant Palmer amaranth (Amaranthus palmeri ). Pest Manag Sci 70:1902–1909 Google Scholar
Paterson, AH, Brubaker, CL, Wendel, JF (1993) A rapid method for extraction of cotton genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep 11:122–127 Google Scholar
Ray, TB (1984) Site of action of chlorsulfuron. Plant Physiol 75:827–831 Google Scholar
Sambrook, J, Fritsch, EF, Maniatis, T (1989) Molecular Cloning: A Laboratory Manual. 2nd edn, Volume 1. Plainview, NY Cold Spring Harbor Laboratory Press. Pp. 82–84 Google Scholar
Shaner, DL, ed (2014) Herbicide Handbook. 10th edn. Lawrence, KS Weed Science Society of America. 513 pGoogle Scholar
Sibony, M, Rubin, B (2003) Molecular basis for multiple resistance to acetolactate synthase-inhibiting herbicides and atrazine in Amaranthus blitoides (prostrate pigweed). Planta 216:1022–1027 Google Scholar
Sosnoskie, LM, Kichler, JM, Wallace, RD, Culpepper, AS (2011) Multiple resistance in Palmer amaranth to glyphosate and pyrithiobac confirmed in Georgia. Weed Sci 59:321–325 Google Scholar
Sprague, CL, Stoller, EW, Wax, LM, Horack, MJ (1997) Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) resistance to selected ALS-inhibiting herbicides. Weed Sci 45:192–197 Google Scholar
Teaster, ND, Hoagland, RE (2014) Characterization of glyphosate resistance in cloned Amaranthus palmeri plants. Weed Biol Manag 14:1–10 Google Scholar
Trucco, F, Jeschke, MR, Rayburn, AL, Tranel, PJ (2005a) Promiscuity in weedy amaranths: high frequency of female tall waterhemp (Amaranthus tuberculatus) × smooth pigweed (A. hybridus) hybridization under field conditions. Weed Sci 53:46–54 Google Scholar
Trucco, F, Rayburn, AL, Tranel, PJ (2005b) Amaranthus hybridus can be pollinated frequently by A. tuberculatus under field conditions. Heredity 94:64–70 Google Scholar
Trucco, F, Tatum, T, Rayburn, AL, Tranel, PJ (2009) Out of the swamp:unidirectional hybridization with weedy species may explain the prevalence of Amaranthus tuberculatus as a weed. New Phytol 184:819–827 Google Scholar
Whaley, CM, Wilson, HP, Westwood, JH (2006) ALS resistance in several smooth pigweed (Amaranthus hybridus) biotypes. Weed Sci 54:828–832 Google Scholar