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Target-Site Resistance to Fenoxaprop-P-ethyl in Keng Stiffgrass (Sclerochloa kengiana) from China

Published online by Cambridge University Press:  24 May 2017

Haitao Gao ,
Jiaxing Yu ,
Lang Pan ,
Xibao Wu  and
Liyao Dong
Haitao Gao
Affiliation:
Graduate Student, Graduate Student, Ph.D Student, Graduate Student, and Professor, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing 210095, China.
Jiaxing Yu
Affiliation:
Graduate Student, Graduate Student, Ph.D Student, Graduate Student, and Professor, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing 210095, China.
Lang Pan
Affiliation:
Graduate Student, Graduate Student, Ph.D Student, Graduate Student, and Professor, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing 210095, China.
Xibao Wu
Affiliation:
Graduate Student, Graduate Student, Ph.D Student, Graduate Student, and Professor, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing 210095, China.
Liyao Dong*
Affiliation:
Graduate Student, Graduate Student, Ph.D Student, Graduate Student, and Professor, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing 210095, China.
*
*Corresponding author's E-mail: [email protected]
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Abstract

Keng stiffgrass is a serious farmland grass weed distributed globally in winter wheat fields and rice–wheat double-cropping areas. The intensive use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has led to the evolution of resistance in a growing number of grass weeds. In this study, whole-plant pot bioassay experiments were conducted to establish that a Keng stiffgrass population from eastern China, JYJD-2, has evolved high-level resistance to fenoxaprop-P-ethyl and moderate resistance to quizalofop-P-ethyl and pinoxaden. Using the derived cleaved amplified polymorphic sequence method, a tryptophan-to-cysteine mutation at codon position 1999 (W1999C) was detected in the ACCase gene of the resistant population JYJD-2. Of the 100 JYJD-2 plants tested, we found 47 heterozygous resistant and 53 homozygous sensitive individuals. In vitro ACCase assays revealed that the IC50 value of the ACCase activity of the resistant population JYJD-2 was 6.48-fold higher than that of the susceptible population JYJD-1. To the best of our knowledge, this is the first report of the occurrence of W1999C mutation in the ACCase gene of fenoxaprop-P-ethyl–resistant Keng stiffgrass. This study confirmed the resistance of Keng stiffgrass to the ACCase inhibitor fenoxaprop-P-ethyl, cross-resistance to other ACCase inhibitors, and the resistance being conferred by specific ACCase point mutations at amino acid position 1999.

Keywords

fenoxaprop-P-ethylquizalofop-P-ethylpinoxadenKeng stiffgrass, Sclerochloa kengiana (Ohwi) Tzvel.rice, Oryza sativa L.wheat, Triticum aestivum L.ACCase activityAcetyl-CoA carboxylasederived cleaved amplified polymorphic sequencePseudosclerochloa kengiana (Ohwi) Tzvel.target-site resistance
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 65 , Issue 4 , July 2017 , pp. 452 - 460
Copyright
© Weed Science Society of America, 2017 

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Footnotes

Associate Editor for this paper: Vijay Nandula, USDA–ARS.

References

Literature Cited

Beckie, HJ, Tardif, FJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:15–28 Google Scholar
Beckie, HJ, Warwick, SI, Sauder, CA (2011) Basis for herbicide resistance in Canadian populations of wild oat (Avena fatua). Weed Sci 60:10–18 CrossRefGoogle Scholar
Bi, Y, Liu, W, Guo, W, Li, L, Yuan, G, Du, L, Wang, J (2016) Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China. Pestic Biochem Physiol 126:22–27 Google Scholar
Cui, H, Li, X, Wang, G, Wang, J, Wei, S, Cao, H (2012) Acetolactate synthase proline (197) mutations confer tribenuron-methyl resistance in Capsella bursa-pastoris populations from China. Pestic Biochem Physiol 102:229–232 CrossRefGoogle Scholar
Délye, C (2005) Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53:728–746 Google Scholar
Délye, C, Calmès, É, Matéjicek, A (2002a) SNP markers for black-grass (Alopecurus myosuroides Huds.) genotypes resistant to acetyl CoA-carboxylase inhibiting herbicides. Theor Appl Genet 104:1114–1120 Google Scholar
Délye, C, Matéjicek, A, Gasquez, J (2002b) PCR-based detection of resistance to acetyl–CoA carboxylase–inhibiting herbicides in black–grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud). Pest Manag Sci 58:474–478 Google Scholar
Délye, C, Pernin, F, Michel, S (2011) “Universal” PCR assays detecting mutations in acetyl-coenzyme A carboxylase or acetolactate synthase that endow herbicide resistance in grass weeds. Weed Res 51:353–362 Google Scholar
Délye, C, Zhang, XQ, Chalopin, C, Michel, S, Powles, SB (2003) An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors. Plant Physiol 132:1716–1723 Google Scholar
Délye, C, Zhang, XQ, Michel, S, Matéjicek, A, Powles, SB (2005) Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass. Plant Physiol 137:794–806 Google Scholar
Heap, I (2016) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: March 16, 2016Google Scholar
Heap, I, Knight, R (1986) The occurrence of herbicide cross-resistance in a population of annual ryegrass (Lolium rigidum) resistant to diclofop-methyl. Aust J Agric Res 37:149–156 CrossRefGoogle Scholar
Hochberg, O, Sibony, M, Rubin, B (2009) The response of ACCase-resistant Phalaris paradoxa populations involves two different target site mutations. Weed Res 49:37–46 CrossRefGoogle Scholar
Jang, S, Marjanovic, J, Gornicki, P (2013) Resistance to herbicides caused by single amino acid mutations in acetyl-CoA carboxylase in resistant populations of grassy weeds. New Phytol 197:1110–1116 Google Scholar
Kaundun, SS (2010) An aspartate to glycine change in the carboxyl transferase domain of acetyl-CoA carboxylase and non-target-site mechanism(s) confer resistance to ACCase inhibitor herbicides in a Lolium multiflorum population. Pest Manag Sci 66:1249–1256 Google Scholar
Kaundun, SS, Bailly, GC, Dale, RP, Hutchings, SJ, McIndoe, E (2013) A novel W1999S mutation and non-target site resistance impact on acetyl-CoA carboxylase inhibiting herbicides to varying degrees in a UK Lolium multiflorum population. PLoS ONE 8:e58012 CrossRefGoogle Scholar
Li, L, Du, L, Liu, W, Yuan, G, Wang, J (2014) Target-site mechanism of ACCase-inhibitors resistance in American sloughgrass (Beckmannia syzigachne Steud.) from China. Pestic Biochem Physiol 110:57–62 CrossRefGoogle ScholarPubMed
Liu, W, Harrison, DK, Chalupska, D, Gornicki, P, O’Donnell, CC, Adkins, SW, Haselkorn, R, Williams, RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci USA 104:3627–3632 Google Scholar
Neff, MM, Turk, E, Kalishman, M (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet 18:613–615 CrossRefGoogle ScholarPubMed
Pan, L, Li, J, Xia, W, Zhang, D, Dong, L (2015a) An effective method, composed of LAMP and dCAPS, to detect different mutations in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations. Pestic Biochem Physiol 117:1–8 Google Scholar
Pan, L, Li, J, Zhang, W, Dong, L (2015c) Detection of the I1781L mutation in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.), based on the loop-mediated isothermal amplification method. Pest Manag Sci 71:123–130 Google Scholar
Pan, L, Li, J, Zhang, T, Zhang, D, Dong, L (2015b) Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne . Weed Res 55:609–620 Google Scholar
Petit, C, Bay, G, Pernin, F, Delye, C (2010) Prevalence of cross- or multiple resistance to the acetyl-coenzyme A carboxylase inhibitors fenoxaprop, clodinafop and pinoxaden in black-grass (Alopecurus myosuroides Huds.) in France. Pest Manag Sci 66:168–177 Google Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347 Google Scholar
Rao, N, Dong, L, Li, J, Zhang, H (2008) Influence of environmental factors on seed germination and seedling emergence of American Sloughgrass (Beckmannia syzigachne). Weed Sci 56:529–533 Google Scholar
Scarabel, L, Panozzo, S, Varotto, S, Sattin, M (2011) Allelic variation of the ACCase gene and response to ACCase-inhibiting herbicides in pinoxaden-resistant Lolium spp. Pest Manag Sci 67:932–941 CrossRefGoogle ScholarPubMed
Shukla, A, Leach, GE, Devine, MD (1997) High-level resistance to sethoxydim conferred by an alteration in the target enzyme, acetyl-CoA carboxylase, in Setaria faberi and Setaria viridis . Plant Physiol Biochem 35:803–807 Google Scholar
Stoltenberg, DE, Gengenbach, BG (1989) Effect of sethoxydim and haloxyfop on acetyl-coenzyme A carboxylase activity in Festuca species. Weed Sci 37:512–516 Google Scholar
Tang, W, Zhou, F, Chen, J, Zhou, X (2014) Resistance to ACCase-inhibiting herbicides in an Asia Minor bluegrass (Polypogon fugax) population in China. Pestic Biochem Physiol 108:16–20 CrossRefGoogle Scholar
Tardif, FJ, Powles, SB (1994) Herbicide multiple-resistance in a Lolium rigidum biotype is endowed by multiple mechanisms: isolation of a subset with resistant acetyl-CoA carboxylase. Physiol Plant 91:488–494 Google Scholar
Warwick, SI, Sauder, CA, Beckie, HJ (2010) Acetolactate synthase (ALS) target-site mutations in ALS inhibitor-resistant Russian thistle (Salsola tragus). Weed Sci 58:244–251 Google Scholar
Xu, H, Li, J, Zhang, D, Cheng, Y, Jiang, Y, Dong, L (2014a) Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus). Pest Manag Sci 70:1894–1901 Google Scholar
Xu, H, Zhang, W, Zhang, T, Li, J, Wu, X, Dong, L (2014b) Determination of ploidy level and isolation of genes encoding acetyl-CoA carboxylase in Japanese foxtail (Alopecurus japonicus). PLoS ONE 9:e114712 Google Scholar
Xu, H, Zhu, X, Wang, H, Li, J, Dong, L (2013) Mechanism of resistance to fenoxaprop in Japanese foxtail (Alopecurus japonicus) from China. Pestic Biochem Physiol 107:25–31 CrossRefGoogle ScholarPubMed
Yang, C, Dong, L, Li, J, Moss, SR (2007) Identification of Japanese foxtail (Alopecurus japonicus) resistant to haloxyfop using three different assay techniques. Weed Sci 55:537–540 Google Scholar
Yu, Q, Ahmad-Hamdani, MS, Han, H, Christoffers, MJ, Powles, SB (2013) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species. Heredity 110:220–231 Google Scholar
Yu, Q, Cairns, A, Powles, S (2007a) Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta 225:499–513 Google Scholar
Yu, Q, Collavo, A, Zheng, MQ, Owen, M, Sattin, M, Powles, SB (2007b) Diversity of acetyl-coenzyme A carboxylase mutations in resistant Lolium populations: evaluation using clethodim. Plant Physiol 145:547–558 Google Scholar
Yuan, G, Liu, W, Bi, Y, Du, L, Guo, W, Wang, J (2015) Molecular basis for resistance to ACCase-inhibiting herbicides in Pseudosclerochloa kengiana populations. Pestic Biochem Physiol 119:9–15 Google Scholar
Zhang, H, Yang, Z, Shen, Y, Tong, L (2003) Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase. Science 299:2064–2067 Google Scholar
Zhang, ZP (2003) Development of chemical weed control and integrated weed management in China. Weed Biol Manag 3:197–203 Google Scholar