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A Simple In-Season Bioassay for Detecting Glyphosate Resistance in Grass and Broadleaf Weeds Prior to Herbicide Application in the Field

Published online by Cambridge University Press:  20 January 2017

Shiv S. Kaundun ,
Sarah-Jane Hutchings ,
Suzanne C. Harris ,
Lucy V. Jackson ,
Rekha Shashi-Kiran ,
Richard P. Dale  and
Eddie McIndoe
Shiv S. Kaundun*
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Sarah-Jane Hutchings
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Suzanne C. Harris
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Lucy V. Jackson
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Rekha Shashi-Kiran
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Richard P. Dale
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
Eddie McIndoe
Affiliation:
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom
*
Corresponding author's E-mail: [email protected]
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Abstract

The implementation of a successful glyphosate resistance management strategy requires a simple and cost-effective method for detecting resistance in key weeds. To date, however, glyphosate resistance is still routinely confirmed via laborious and time consuming whole-plant pot assays using seeds collected at the end of the growing season. Here, we describe a simple, early-season bioassay for detecting evolved glyphosate resistance in grass and broadleaf weeds. It involves transplanting suspected glyphosate resistant seedlings alongside known sensitive and resistant standards into agar containing informative rates of herbicide and recording percentage survival 14 d after plating. The method was validated using sensitive and resistant populations of Lolium, Eleusine, Conyza, and Amaranthus species encompassing the main glyphosate resistance mechanisms, namely, impaired translocation, EPSPS gene duplication, and mutations. The whole plant pot and agar-based seedling tests generated comparable resistance indices in dose-response assays and percentage survival at discriminating glyphosate rates. The method was applied successfully to detect resistance in a rigid ryegrass population collected from a French vineyard well before glyphosate was applied in the field for the current season. Additionally, the test was shown to be highly transferable to several other grass and broadleaf weeds that have evolved resistance to glyphosate. One major attribute of the method is that it is capable of detecting resistance regardless of the mechanism involved. In addition to being very simple, quick and, cost-effective, it allows determination of glyphosate resistance in weeds prior to field application. It thus offers the opportunity for an informed choice of herbicides for effective weed control.

Keywords

Glyphosatehorseweed, Conyza canadensis (L.) Cronq. ERICAItalian ryegrass, Lolium multiflorum Lam. LOLMUrigid ryegrass, Lolium rigidum (Gaudin)Eleusine indica (L.) Gaertn. ELEINcommon waterhemp, Amaranthus rudis J.D. SauerAMATA.Weed resistanceagar-based bioassayin-season resistance detection method
Type
Weed Management
Information
Weed Science , Volume 62 , Issue 4 , December 2014 , pp. 597 - 607
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Baerson, SR, Rodriguez, DJ, Tran, M, Feng, Y, Biest, NA, Dill, GM (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265–1275 Google Scholar
Baylis, AD (2000) Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag Sci. 56:299–308 Google Scholar
Beckie, H, Blackshaw, R, Low, R, Hall, L, Sauder, C, Martin, S, Brandt, R, Shirriff, S (2013) Glyphosate- and acetolactate synthase inhibitor–resistant Kochia (Kochia scoparia) in Western Canada. Weed Sci. 61:310–318 Google Scholar
Burgos, N, Tranel, PG, Strebig, JC, Davis, VM, Shaner, D, Norsworthy, JK, Ritz, C (2013) Review: confirmation of resistance to herbicides and evaluation of resistance levels. Weed Sci. 61:4–20 Google Scholar
Collavo, A, Sattin, M (2012) Resistance to glyphosate in Lolium rigidum selected in Italian perennial crops: bioevaluation, management and molecular bases of target-site resistance. Weed Res. 52:16–24 Google Scholar
Culpepper, AS, Grey, TL, Vencill, WK, Kichler, JM, Webster, TM, Brown, SM, York, AC, Davis, JW, Hanna, WW (2006) Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia. Weed Sci. 54:620–626 Google Scholar
de Carvalho, L, Cruz-Hipolito, H, González-Torralva, F, da Costa, P, Alves, A, Christoffoleti, P, de Prado, R (2011) Detection of sourgrass (Digitaria insularis) biotypes resistant to glyphosate in Brazil. Weed Sci. 52:171–176 Google Scholar
Dinelli, G, Marotti, I, Bonetti, A, Minelli, M, Catizone, P, Barnes, J (2006) Physiological and molecular insight on the mechanisms of resistance to glyphosate in Conyza canadensis (L.) cronq. biotypes. Pestic Biochem Physiol. 86:30–41 Google Scholar
Duke, S, Powles, S (2008) Glyphosate: A once-in-a-century herbicide. Pest Manag Sci. 64:319–325 Google Scholar
Duke, S, Powles, S (2009) Glyphosate-resistant crops and weeds; now and in the future. AgBioForum. 12:346–357 Google Scholar
Finney, DJ (1971) Probit analysis. 3rd ed. Cambridge Cambridge University Press. 333 pGoogle Scholar
Franz, J, Mao, M, Sikorski, J (1997) Glyphosate: A unique global herbicide. Washington, D.C. American Chemical Society. 653 pGoogle Scholar
Gaines, T, Cripps, A, Powles, S (2012) Evolved resistance to glyphosate in Junglerice (Echinochloa colona) from the tropical Ord river region in Australia. Weed Technol. 26:480–484 Google Scholar
Gaines, T, Zhang, W, Wang, D, Bukuna, B, Chisholm, S, Shaner, D, Nissen, S, Patzoldt, W, Tranel, P, Culpepper, A, Grey, T, Websterg, T, Vencill, W, Sammons, R, Jiang, J, Preston, C, Leach, J, Westra, P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci USA. 107:1029–1034 Google Scholar
Heap, IM (2014) International Survey of Herbicide-Resistant Weeds. http://www.weedscience.com. Accessed March 27, 2014Google Scholar
Jaworski, EG (1972) Mode of action of N-phosphonomethylglycine. Inhibition of aromatic amino acid biosynthsis. J Agric Food Chem. 20:1195–1198 Google Scholar
Kaundun, SS, Dale, R, Zelaya, , Dinelli, G, Maroti, I, Cairns, A, McIndoe, E (2011) A novel P106L mutation in the EPSPS target gene contributes to glyphosate resistance in a Lolium population. J Sci Agric Food Chem. 59:3227–3233 Google Scholar
Kaundun, SS, Hutchings, S-J, Bailly, G, Dale, R, Glanfield, P (2010) Syngenta ‘RISQ’ test: a novel in-season method for detecting resistance to post-emergence ACCase and ALS inhibitor herbicides in grass weeds. Weed Res. 51:284–293 Google Scholar
Kaundun, SS, Zelaya, I, Dale, R, Lycett, A, Carter, P, Sharples, P, McIndoe, E (2008) Importance of the P106S target site mutation in conferring resistance to glyphosate in an Eleusine indica biotype from the Philippines. Weed Sci. 56:637–646 Google Scholar
Koger, CH, Poston, DH, Hayes, RM, Montgomery, RF (2004) Glyphosate-resistant horseweed (Conyza canadensis) in Mississippi. Weed Technol. 18:820–825 Google Scholar
Koger, CH, Shaner, DL, Henry, WB, Nadler-Hassar, T, Thomas, WE, Wilcut, JW (2005) Assessment of two nondestructive assays for detecting glyphosate resistance in horseweed (Conyza canadensis). Weed Sci. 53:559–566 Google Scholar
Mohseni-Moghadam, M, Schroeder, J, Ashigh, J (2013) Mechanism of resistance and inheritance in glyphosate resistant Palmer amaranth (Amaranthus palmeri) populations from New Mexico, USA. Weed Sci. 27:85–91 Google Scholar
Mueller, TC, Massey, JH, Hayes, RM, Main, CL, Stewart, CNJ (2003) Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq.). J Agric Food Chem. 51:680–684 Google Scholar
Nandula, V, Ray, J, Ribeiro, D, Pan, Z, Reddy, K (2013) Glyphosate resistance in Tall Waterhemp (Amaranthus tuberculatus) from mississippi is due to both altered target site and non-target site mechanisms. Weed Sci. 61:374–383 Google Scholar
Ng, CH, Wickneswary, R, Salmijah, S, Teng, YT, Ismail, BS (2004) Glyphosate resistance in Eleusine indica (L.) Gaertn. from different origins and polymerase chain reaction amplification of specific alleles. Aust J Agric Res. 55:407–414 Google Scholar
Nol, L, Tsikou, D, Eid, M, Livieratos, I, Giannopolitis, C (2012) Shikimate leaf disc assay for early detection of glyphosate resistance in Conyza canadensis and relative transcript levels of EPSPS and ABC transporter genes. Weed Res. 52:233–241 Google Scholar
Norsworthy, JK (2013) Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) in Southern USA row-crop production: impact and current management strategies. Pages 92 in Proceedings of the Global Herbicide Resistance Challenge Conference. Perth, Australia.Google Scholar
Owen, MDK, Zelaya, IA (2005) Herbicide-resistant crops and weed resistance to herbicides. Pest Manag Sci. 61:301–311 Google Scholar
Perez-Jones, A, Mallory-Smith, C (2010) Biochemical mechanisms and molecular basis of evolved glyphosate resistance in weed species. Pages 119–140 in Nandula, VK, ed. Glyphosate resistance in crops and weeds: history, development and management. New Jersey Wiley Google Scholar
Perez-Jones, A, Park, K-W, Polge, N, Colquhoun, J, Mallory-Smith, CA (2007) Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta. 226:395–404 Google Scholar
Powles, S, Yu, Q (2010) Evolution in action: plants reistant to herbicides. Ann Rev Plant Biol. 61:317–47Google Scholar
Pratley, J, Baines, P, Eberbach, R, Incerti, M, Broster, J (1996) Glyphosate resistance in annual ryegrass. Pages 126 in Proceedings of the 11th annual conference of the grasslands society of the New South Wales. Wagga Wagga, Australia The Grasslands Society Google Scholar
Preston, C, Wakelin, AM (2008) Resistance to glyphosate from altered herbicide translocation patterns. Pest Manag Sci. 64:372–376 Google Scholar
Sammons, DR, Shaner, DL, Remucal, D, Kretzmer, K, Dejarnette, R (2013) A rapid enzyme assay for shikimate to detect glyphosate resistance in the field. Pages 42 in Proceedings of the Global Herbicide Resistance challenge. Perth, Australia.Google Scholar
Sammons, R, Heering, D, Danicola, N, Glick, H, Elmore, G (2007) Sustainability and stewardship of glyphosate and glyphosate-resistant crops. Weed Technol. 21:347–354 Google Scholar
Shaner, D (2010) Testing methods for glyphosate resistance. Pages 93–118 in Nandula, VK, ed. Glyphosate resistance in crops and weeds: history, development and management. New Jersey Wiley Google Scholar
Shrestha, A, Hembreee, K, Va, N (2007) Growth stage influences level of resistance in glyphosate-resistant horseweed. California Agriculture. 61:67–70 Google Scholar
Steinrücken, HC, Amrhein, N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem Biophys Res Commun. 94:1207–1212 Google Scholar
Tranel, P, Riggins, C, Bell, M, Hager, A (2011) Herbicide resistances in Amaranthus tuberculatus: a call for new options. J Agric Food Chem. 59:5808–5812 Google Scholar
VanGessel, MJ (2001) Glyphosate-resistant horseweed from Delaware. Weed Sci. 49:703–705 Google Scholar
Vink, J, Soltani, N, Robinson, D, Tardif, F, Lawton, M, Sikkema, P (2012) Occurrence and distribution of glyphosate-resistant giant ragweed (Ambrosia trifida L.) in southwestern Ontario. Can J Plant Sci. 92:533–539 Google Scholar
Wakelin, AM, Preston, C (2006) A target-site mutation is present in a glyphosate resistant Lolium rigidum population. Weed Res. 46:432–440 Google Scholar
Westhoven, A, Davis, V, Gibson, K, Weller, S, Johnson, W (2008) Field presence of glyphosate-resistant horseweed (Conyza canadensis), common lambsquarters (Chenopodium album) and giant ragweed (Ambrosia trifida) biotypes with elevated tolerance to glyphosate. Weed Tech. 22:544–548 Google Scholar
Zelaya, IA, Owen, MDK (2005) Differential response of Amaranthus tuberculatus (Moq ex DC) JD Sauer to glyphosate. Pest Manag Sci. 61:936–950 Google Scholar