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RAPD analysis of cultivated and wild yellow nutsedge (Cyperus esculentus L.)

Published online by Cambridge University Press:  12 June 2017

Paloma Abad ,
Bernardo Pascual ,
José V. Maroto ,
Salvador López-Galarza ,
María J. Vicente  and
José Alagarda
Paloma Abad
Affiliation:
Departamento Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera, 15, 46020 Valencia, Spain
José V. Maroto
Affiliation:
Departamento Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera, 15, 46020 Valencia, Spain
Salvador López-Galarza
Affiliation:
Departamento Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera, 15, 46020 Valencia, Spain
María J. Vicente
Affiliation:
Departamento Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera, 15, 46020 Valencia, Spain
José Alagarda
Affiliation:
Departamento Producción Vegetal, Universidad Politécnica de Valencia, Camino de Vera, 15, 46020 Valencia, Spain
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Abstract

Cultivated and weedy clones of yellow nutsedge were analyzed using random amplified polymorphic DNA (RAPD) markers to assess the polymorphism within the species and determine if this approach was suitable for identification of cultivar and wild populations. The RAPD markers unambiguously identified all studied clones. Nei-Li similarities were computed and used in an unweighted pair group method using arithmetic average (UPGMA) cluster analyses. Cultivated and weedy clones were clustered in two groups, but two cultivated clones were more closely related to weedy clones than to cultivated clones. The results showed a high level of genetic variability among the clones tested, particularly among the cultivated ones. Identification of yellow nutsedge cultivars and analysis of genetic diversity within and among weedy populations is possible by using only a small number of primers. In this study, seven selected primers discriminated among the 10 tested clones.

Keywords

CYPES genetic markersfingerprintingsimilarity coefficientcultivar identificationgenetic variation
Type
Weed Biology and Ecology
Information
Weed Science , Volume 46 , Issue 3 , June 1998 , pp. 318 - 321
Copyright
Copyright © 1998 by the Weed Science Society of America 

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