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Phytoene Desaturase, the Essential Target for Bleaching Herbicides

Published online by Cambridge University Press:  12 June 2017

Gerhard Sandmann ,
Arno Schmidt ,
Hartmut Linden  and
Peter Böger
Gerhard Sandmann
Affiliation:
Univ. Konstanz, Konstanz, Germany
Arno Schmidt
Affiliation:
Univ. Konstanz, Konstanz, Germany
Hartmut Linden
Affiliation:
Univ. Konstanz, Konstanz, Germany
Peter Böger
Affiliation:
Univ. Konstanz, Konstanz, Germany
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Abstract

Many bleaching herbicides with different core structures inhibit phytoene desaturase (PD), a membrane-bound enzyme in the carotenogenic pathway catalyzing the hydrogen abstraction step at the first C40 precursor of β-carotene. Prospects are good that new PD-active herbicides will be discovered by screening for bleaching activity. Accordingly, interest in PD enzymology and molecular genetics has increased. Although active carotenogenic cell-free systems are available, no isolation of PD has been achieved since the enzyme cannot be detected in its isolated form due to complete loss of activity. A portion of the Rhodobacter PD gene was incorporated into an appropriate plasmid which could be expressed in E. coli. This system was used to produce an antibody specific against PD from higher plants as well as Rhodobacter. All PDs assayed had an apparent molecular weight of 52 to 55 kDa. A Rhodobacter gene probe hybridized with a 3.1 kbBamH I fragment from Aphanocapsa which allowed us to sequence the PD gene from this cyanobacterium. Its DNA sequence matched with the apparent molecular weight of the PD band in the western blot, and a fusion-gene product was found to be immunoreactive with the Rhodobacter PD antibody, Anacystis mutants were produced exhibiting cross-resistance against norflurazon and fluorochloridone. Apparently, this resistance is due to an altered PD with concurrent decrease of inhibitor binding affinity. Cloning of the resistant gene into the wild type is in progress.

Keywords

Herbicide resistancecell-free carotenogenic assaysphytoene desaturase
Type
Special Topics
Information
Weed Science , Volume 39 , Issue 3 , September 1991 , pp. 474 - 479
Copyright
Copyright © 1991 by the Weed Science Society of America 

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References

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