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Phosphate Status Affects Phosphate Transporter Expression and Glyphosate Uptake and Transport in Grand Eucalyptus (Eucalyptus grandis)

Published online by Cambridge University Press:  03 October 2018

Fernanda Campos Mastrotti Pereira ,
Reuben Tayengwa ,
Pedro Luis Da Costa Aguiar Alves  and
Wendy Ann Peer Open the ORCID record for Wendy Ann Peer [Opens in a new window]
Fernanda Campos Mastrotti Pereira
Affiliation:
Doctoral Student, Department of Applied Biology to Agropecuary, São Paulo State University, Jaboticabal, Brasil; and Department of Environmental Science and Technology, University of Maryland, College Park, MD, USA
Reuben Tayengwa
Affiliation:
Postdoctoral Fellow, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA
Pedro Luis Da Costa Aguiar Alves
Affiliation:
Professor, Department of Applied Biology to Agropecuary, São Paulo State University, Jaboticabal, Brasil
Wendy Ann Peer*
Affiliation:
Assistant Professor, Department of Environmental Science and Technology, University of Maryland, College Park, MD, USA; and Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA; and Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA
*
Author for correspondence: W. A. Peer, Department of Environmental Science and Technology, University of Maryland, College Park, MD 20742. (Email: [email protected])
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Abstract

Soluble phosphate availability is a major limiting factor for plant growth, development, and yield. To assure a constant phosphorous supply, plants employ both high- and low-affinity phosphate acquisition mechanisms. Glyphosate is an herbicide widely used throughout the world, and previous studies have suggested that it can be transported across the plasma membrane via phosphate transporters in herbaceous species. The effects of phosphate status on glyphosate uptake were investigated in the tree grand eucalyptus (Eucalyptus grandis W. Hill ex. Maid.). Eucalyptus grandis’s putative phosphate transporters showed differential gene expression in leaves and roots in response to phosphate limitation. Overall, the expression of high-affinity phosphate transporters increased in phosphate-limiting treatments, particularly in roots. More [14C]glyphosate was absorbed and translocated in phosphate-limiting plants compared with control plants grown in phosphate-replete treatments. In leaf mesophyll protoplast assays, rapid uptake of [14C]glyphosate into protoplasts was observed, and addition of phosphate to the assays competed with [14C]glyphosate uptake. These data indicate that phosphate transporters represent one mechanism of glyphosate uptake in E. grandis. These results have implications for best management practices for weed control and glyphosate application under phosphate application regimes.

Keywords

Vijay Nandula, USDA–ARSNutrient managementphosphorous
Type
Research Article
Information
Weed Science , Volume 67 , Issue 1 , January 2019 , pp. 29 - 40
Copyright
© Weed Science Society of America, 2018 

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