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Molecular Analysis of Hexazinone-Resistant Shepherd's-Purse (Capsella bursa-pastoris) Reveals a Novel psbA Mutation

Published online by Cambridge University Press:  20 January 2017

Alejandro Perez-Jones ,
Suphannika Intanon  and
Carol Mallory-Smith
Alejandro Perez-Jones
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Suphannika Intanon
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Carol Mallory-Smith*
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
*
Corresponding author's E-mail: [email protected]
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Abstract

A population of shepherd's-purse suspected to be resistant to the triazinone herbicide hexazinone, a photosystem II (PS II) inhibitor, was collected from an alfalfa field in 2007 in Oregon. A whole-plant, dose–response assay confirmed that the putative-resistant population was highly resistant to hexazinone. The resistant population was 22-fold more resistant to hexazinone than the susceptible population. However, the hexazinone-resistant population was susceptible to other PS II-inhibiting herbicides, including atrazine, diuron, and terbacil. DNA sequence analysis of the chloroplast psbA gene encoding the D1 protein of PS II, the target site of PS II inhibitors, identified a point mutation from Phe to Ile at position 255 in the hexazinone-resistant population. Single- and double-point mutations at position 255, which is located in the QB binding niche of the D1 protein, were previously reported in Chlamydomonas reinhardtii, Synechococcus species, and Synechocystis species after site-directed mutagenesis and were associated with decreased binding of PS II inhibitors. To our knowledge, this is the first report of a mutation of the psbA gene at Phe255 in a field-selected, herbicide-resistant plant.

Keywords

Atrazinediuronhexazinonemetribuzinterbacilalfalfa, Medicago sativa L. MEDSAshepherd's-purse, Capsella bursa-pastoris (L.) Medik CAPBP.PS II inhibitorsphotosynthesispsbAhexazinone-resistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 57 , Issue 6 , December 2009 , pp. 574 - 578
Copyright
Copyright © Weed Science Society of America 

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