Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2025-01-03T16:54:51.520Z Has data issue: false hasContentIssue false
  • English
  • Français

Glyphosate-Resistant Italian Ryegrass (Lolium multiflorum) in California: Distribution, Response to Glyphosate, and Molecular Evidence for an Altered Target Enzyme

Published online by Cambridge University Press:  20 January 2017

Marie Jasieniuk ,
Riaz Ahmad ,
Anna M. Sherwood ,
Jeffrey L. Firestone ,
Alejandro Perez-Jones ,
W. Thomas Lanini ,
Carol Mallory-Smith  and
Zachary Stednick
Marie Jasieniuk*
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
Riaz Ahmad
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
Anna M. Sherwood
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
Jeffrey L. Firestone
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
Alejandro Perez-Jones
Affiliation:
Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002
W. Thomas Lanini
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
Carol Mallory-Smith
Affiliation:
Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002
Zachary Stednick
Affiliation:
Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616-8780
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Selection by herbicides has resulted in widespread evolution of herbicide resistance in agricultural weeds. In California, resistance to glyphosate was first confirmed in rigid ryegrass in 1998. Objectives of this study were to determine the current distribution and level of glyphosate resistance in Italian ryegrass, and to assess whether resistance could be due to an altered target site. Seeds were sampled from 118 populations and seedlings were treated with glyphosate at 866 g ae ha−1. Percentage of survivors ranged from 5 to 95% in 54 populations. All plants from 64 populations died. One susceptible (S) population, four putatively resistant (R) populations, and one S accession from Oregon were used for pot dose–response experiments, shikimic acid analyses, and DNA sequencing. Seedlings were treated with glyphosate at eight rates, ranging from 108 to 13,856 g ae ha−1. Shoot biomass was evaluated 3 wk after treatment and fit to a log-logistic regression equation. On the basis of GR50 (herbicide rate required to reduce growth by 50%) values, seedlings from putatively R populations were roughly two to 15 times more resistant to glyphosate than S plants. Shikimic acid accumulation was similar in all plants before glyphosate treatment, but at 4 and 7 DAT, S plants from California and Oregon accumulated approximately two and three times more shikimic acid, respectively, than R plants. Sequencing of a cDNA fragment of the EPSPS coding region revealed two different codons, both of which encode proline at amino acid position 106 in S individuals. In contrast, all R plants sequenced exhibited missense mutations at site 106. Plants from one population revealed a mutation resulting in a proline to serine substitution. Plants from three R populations exhibited a mutation corresponding to replacement of proline with alanine. Our results indicate that glyphosate resistance is widespread in Italian ryegrass populations of California, and that resistance is likely due to an altered target enzyme.

Keywords

GlyphosateItalian ryegrass, Lolium multiflorum Lam. LOLMUrigid ryegrass, Lolium rigidum Gaud. LOLRIGlyphosate resistanceherbicide resistance5-enolpyruvylshikimate-3-phosphate synthaseEPSPSshikimic acidtarget site
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 4 , August 2008 , pp. 496 - 502
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Baerson, S. R., Rodriguez, D. J., Tran, M., Feng, Y., Biest, N. A., and Dill, G. M. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265–1275.CrossRefGoogle ScholarPubMed
Baylis, A. D. 2000. Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag. Sci. 56:299–308.3.0.CO;2-K>CrossRefGoogle Scholar
Christoffers, M. J. 1999. Genetic aspects of herbicide-resistant weed management. Weed Technol. 13:647–652.CrossRefGoogle Scholar
Comai, L., Sen, L. C., and Stalker, D. M. 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science. 221:370–371.CrossRefGoogle Scholar
Cromartie, T. H. and Polge, N. D. 2000. An improved assay for shikimic acid and its use as a monitor for the activity of sulfosate. Proc. Weed Sci. Soc. Am. 40:291.Google Scholar
Gressel, J. 2002. Evolution of resistance to herbicides. Pages 78–121. in Gressel, J. Molecular Biology of Weed Control. New York Taylor & Francis.CrossRefGoogle Scholar
Heap, I. 2007. International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: November 6, 2007.Google Scholar
Herrmann, K. M. and Weaver, L. M. 1999. The shikimate pathway. Annu. Rev. Plant Physiol. Mol. Biol. 50:473–503.CrossRefGoogle ScholarPubMed
Jasieniuk, M., Brûlé-Babel, A. L., and Morrison, I. N. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Sci. 44:176–193.CrossRefGoogle Scholar
Jaworski, E. G. 1982. Mode of action of N-phosphonomethylglycine: inhibition of aromatic amino acid biosynthesis. J. Agric. Food Chem. 20:1195–1198.CrossRefGoogle Scholar
Lee, L. J. and Ngim, J. 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica (L) Gaertn) in Malaysia. Pest Manag. Sci. 56:336–339.3.0.CO;2-8>CrossRefGoogle Scholar
Lorraine-Colwill, D. F., Powles, S. B., Hawkes, T. R., Hollinshead, P. H., Warner, S. A. J., and Preston, C. 2002. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol. 74:62–72.CrossRefGoogle Scholar
Maxwell, B. D., Roush, M. L., and Radosevich, S. R. 1990. Predicting the evolution and dynamics of herbicide resistance in weed populations. Weed Technol. 4:2–13.CrossRefGoogle Scholar
Ng, C. H., Wickneswary, R., Salmijah, S., Teng, Y. T., and Ismail, B. S. 2003. Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Res. 43:108–115.CrossRefGoogle Scholar
Ng, C. H., Wickneswary, R., Salmijah, S., Teng, Y. T., and Ismail, B. S. 2004. Glyphosate resistance in Eleusine indica (L.) Gaertn. from different origins and polymerase chain reaction amplification of specific alleles. Aust. J. Agric. Res. 55:407–414.CrossRefGoogle Scholar
Padgette, S. R., Re, D. B., Gasser, C. S., Eichholtz, D. A., Frazier, R. B., Hironaka, C. M., Levine, E. B., Shah, D. M., Fraley, R. T., and Kishore, G. M. 1991. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. J. Biol. Chem. 266:22,364–22,369.CrossRefGoogle ScholarPubMed
Pedersen, B. P., Neve, P., Andreasen, C., and Powles, S. B. 2007. Ecological fitness of a glyphosate-resistant Lolium rigidum population: growth and seed production along a competition gradient. Basic Appl. Ecol. 8:258–268.CrossRefGoogle Scholar
Perez, A. and Kogan, M. 2003. Glyphosate-resistant Lolium multiflorum in Chilean orchards. Weed Res. 43:12–19.CrossRefGoogle Scholar
Perez-Jones, A., Park, K-W., Colquhoun, J., Mallory-Smith, C., and Shaner, D. 2005. Identification of glyphosate-resistant Italian ryegrass (Lolium multiflorum) in Oregon. Weed Sci. 53:775–779.CrossRefGoogle Scholar
Perez-Jones, A., Park, K-W., Polge, N., Colquhoun, J., and Mallory-Smith, C. A. 2007. Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta. 226:395–404.CrossRefGoogle ScholarPubMed
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci. 46:604–607.CrossRefGoogle Scholar
Powles, S. B. and Preston, C. 2006. Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technol. 20:282–289.CrossRefGoogle Scholar
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci. 47:405–411.CrossRefGoogle Scholar
Preston, C. and Wakelin, A. M. 2008. Resistance to glyphosate from altered herbicide translocation patterns. Pest Manag. Sci. Early View, DOI: 10.1002/ps.1489.CrossRefGoogle Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose–response relationships. Weed Technol. 9:218–225.CrossRefGoogle Scholar
Siehl, D. L. 1997. Inhibitors of EPSPS synthase, glutamine synthetase and histidine synthesis. Pages 37–67. in Roe, R. M., Burton, J. D., and Kuhr, R. J. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam IOS.Google Scholar
Simarmata, M., Kaufmann, J. E., and Penner, D. 2003. Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci. 51:678–682.CrossRefGoogle Scholar
Singh, B. K. and Shaner, D. L. 1998. Rapid determination of glyphosate injury to plants and identification of glyphosate-resistant plants. Weed Technol. 12:527–530.CrossRefGoogle Scholar
Steinrücken, H. C. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid 3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:1207–1212.CrossRefGoogle ScholarPubMed
Streibig, J. C. 1988. Herbicide bioassay. Weed Res. 28:479–484.CrossRefGoogle Scholar
Streibig, J. C., Rudemo, M., and Jensen, J. E. 1993. Dose–response curves and statistical models. Pages 29–56. in Streibig, J. C. and Kudsk, P. Herbicide Bioassays. Boca Raton, FL CRC.Google Scholar
Vila-Aiub, M. M., Balbi, M. C., Gundel, P. E., Ghersa, C. M., and Powles, S. B. 2007. Evolution of glyphosate-resistant Johnsongrass (Sorghum halepense) in glyphosate-resistant soybean. Weed Sci. 55:566–571.CrossRefGoogle Scholar
Wakelin, A. M., Lorraine-Colwill, D. F., and Preston, C. 2004. Glyphosate resistance in four different populations of Lolium rigidum is associated with reduced translocation of glyphosate to meristematic zones. Weed Res. 44:453–459.CrossRefGoogle Scholar
Wakelin, A. M. and Preston, C. 2006. A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432–440.CrossRefGoogle Scholar
Woodburn, A. T. 2000. Glyphosate: production, pricing and use worldwide. Pest Manag. Sci. 56:309–312.3.0.CO;2-C>CrossRefGoogle Scholar
Yu, Q., Cairns, A., and Powles, S. 2007. Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta. 225:499–513.CrossRefGoogle Scholar