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Genetic Variation in Invasive Populations of Yellow Toadflax (Linaria vulgaris) in the Western United States

Published online by Cambridge University Press:  20 January 2017

Sarah M. Ward ,
Scott D. Reid ,
Judy Harrington ,
Jason Sutton  and
K George Beck
Sarah M. Ward*
Affiliation:
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523
Scott D. Reid
Affiliation:
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523
Judy Harrington
Affiliation:
Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523
Jason Sutton
Affiliation:
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523
K George Beck
Affiliation:
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523
*
Corresponding author's E-mail: [email protected]
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Abstract

Intraspecific genetic variation may contribute significantly to invasiveness and control problems, but has been characterized to date in relatively few invasive weed species. We examined 56 intersimple sequence repeat (ISSR) loci in 220 individuals from 11 invading populations of yellow toadflax sampled across five western states. All populations showed high levels of genetic diversity. Estimated values for Shannon's diversity measure ranged from 0.217 to 0.388, and for expected heterozygosity from 0.178 to 0.260. Nei's total gene diversity index (HT), on the basis of all individuals across all populations, was 0.267. Partitioning of genetic variance using analysis of molecular variance revealed 1.7% of genetic variation among regional population groups, 29.1% among populations within groups, and 69.2% within populations, consistent with expectations for an outcrossing species but suggesting little geographic differentiation. Pairs of adjacent individuals identical at all ISSR loci that appeared to be ramets of a single clone were detected in only one population. This indicates that patch expansion in yellow toadflax is driven more by sexual reproduction via seed than by rhizomatous clonal spread, at least at the spatial scale of sampling for this study. Eight populations had significant values for Mantel's R at P = 0.05, suggesting some fine-scale positive genetic structuring, possibly from restricted gene flow. Population clustering on the basis of Nei's genetic distance between populations and unweighted pair group method with arithmetic mean did not reflect geographic location. It is likely that multiple introductions of this species have occurred across the Intermountain West, followed by extensive genetic recombination. High levels of genetic diversity within yellow toadflax populations pose management challenges, as already seen in reports of variable response to herbicide application and limited impacts of biocontrol agent releases.

Keywords

Yellow toadflax, Linaria vulgaris Miller.Genetic diversityintersimple sequence repeatsISSRinvasive plant
Type
Weed Biology and Ecology
Information
Weed Science , Volume 56 , Issue 3 , June 2008 , pp. 394 - 399
Copyright
Copyright © Weed Science Society of America 

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