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Effects of the fungal protein Nep1 and Pseudomonas syringae on growth of Canada thistle (Cirsium arvense), common ragweed (Ambrosia artemisiifolia), and common dandelion (Taraxacum officinale)

Published online by Cambridge University Press:  20 January 2017

John W. Gronwald ,
Kathryn L. Plaisance  and
Bryan A. Bailey
Kathryn L. Plaisance
Affiliation:
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108
Bryan A. Bailey
Affiliation:
Alternate Crops and Systems Laboratory, USDA-ARS, Beltsville, MD 20705
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Abstract

The effects of the fungal protein Nep1 and Pseudomonas syringae pv. tagetis (Pst) applied separately or in combination on Canada thistle, common ragweed, and common dandelion were examined in growth chamber experiments. Experiments examined five treatments: (1) untreated control, (2) Silwet L-77 (0.3%, v/v) control, (3) Nep1 (5 μg ml−1) plus Silwet L-77 (0.3%, v/v), (4) Pst (109 colony-forming units [cfu] ml−1) plus Silwet L-77 (0.3%, v/v), and (5) Pst (109 cfu ml−1) and Nep1 (5 μg ml−1) plus Silwet L-77 (0.3%, v/v). Foliar treatments were applied at 28, 26, and 21 d after planting for Canada thistle, common dandelion, and common ragweed, respectively. For all three species, foliar application of Nep1 alone or in combination with Pst caused rapid desiccation and necrosis of leaves, with the greatest effect on recent, fully expanded (RFE) leaves. Within 4 to 8 h after treatment (HAT), 60 to 80% of RFE leaves of all three species were necrotic. Measured 72 HAT, Pst populations in Canada thistle leaves treated with Nep1 plus Pst were approximately 105 cfu cm−2 compared with 107 cfu cm−2 for leaves treated with Pst alone. Measured 2 wk after treatment, foliar application of Nep1 reduced shoot dry weight of the three weeds by 30 to 41%. Treatment with Pst reduced shoot growth of common ragweed, Canada thistle, and common dandelion by 82, 31, and 41%, respectively. The large suppression of common ragweed shoot growth caused by Pst treatment was associated with a high percentage (60%) of leaf area exhibiting chlorosis. Treatment with Pst plus Nep1 did not result in significant decreases in shoot dry weight for Canada thistle and common dandelion compared with either treatment alone. For common ragweed, shoot growth reduction caused by applying Pst and Nep1 together was not greater than that caused by Pst alone.

Keywords

Canada thistle, Cirsium arvense L. (Scop.) CIRARcommon ragweed, Ambrosia artemisiifolia L. AMBELcommon dandelion, Taraxacum officinale Weber in Wiggers TAROFBioherbicideweed biocontrol
Type
Weed Management
Information
Weed Science , Volume 52 , Issue 1 , February 2004 , pp. 98 - 104
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Bailey, B. A. 1995. Purification of a protein from culture filtrates of Fusarium oxysporum that induces ethylene and necrosis in leaves of Erythroxylum coca . Phytopathology 85:1250–1255.Google Scholar
Bailey, B. A., Apel-Birkhold, P. C., Akingbe, O. O., Ryan, J. L., O'Neill, N. R., and Anderson, J. D. 2000a. Nep1 protein from Fusarium oxysporum enhances biological control of opium poppy by Pleospora papaveracea . Phytopathology 90:812–818.Google Scholar
Bailey, B. A., Collins, R., and Anderson, J. D. 2000b. Factors influencing the herbicidal activity of Nep1, a fungal protein that induces the hypersensitive response in Centaurea maculosa . Weed Sci 48:776–785.Google Scholar
Bailey, B. A., Jennings, J. C., and Anderson, J. D. 1997a. The 24-kDa protein from Fusarium oxysporum f. sp. erythroxyli: occurrence in related fungi and the effect of growth medium on its production. Can. J. Microbiol 43:45–55.Google Scholar
Bailey, B. A., Jennings, J. C., and Anderson, J. D. 1997b. Sensitivity of coca (Erythroxylum coca var. coca) to ethylene and fungal proteins. Weed Sci 45:716–721.Google Scholar
Bailey, K. L., Boyetchko, S. M., Derby, J., Hall, W., Sawchyn, K., Nelson, T., and Johnson, D. R. 2000c. Evaluation of fungal and bacterial agents for biological control of Canada thistle. Pages 203–208 in Spencer, N. R. ed. Proceedings of the 10th International Symposium on Biological Control of Weeds. Bozeman, MT: Montana State University.Google Scholar
Christy, A. L., Herbst, K. A., Kostka, S. J., Mullen, J. P., and Carlson, P. S. 1993. Synergizing weed biocontrol agents with chemical herbicides. Pages 87–100 in Duke, S. O., Menn, J. J., and Plimmer, J. R. eds. ACS Symposium Series 524. Washington, DC: American Chemical Society.Google Scholar
Durbin, R. D. 1990. Biochemistry of non-host-selective phytotoxins. Pages 63–71 in Hoagland, R. E. ed. Microbes and Microbial Products as Herbicides. ACS Symposium Series 439. Washington, DC: American Chemical Society.CrossRefGoogle Scholar
Gronwald, J. W., Plaisance, K. L., Ide, D. A., and Wyse, D. L. 2002. Assessment of Pseudomonas syringae pv. tagetis as a biocontrol agent for Canada thistle. Weed Sci 50:397–404.Google Scholar
Hoagland, D. R. and Arnon, D. I. 1950. The Water-Culture Method for Growing Plants without Soil. University of California Agricultural Experiment Station Circular 347. 32 p.Google Scholar
Hoeft, E. V., Jordon, N., Zhang, J., and Wyse, D. L. 2001. Integrated cultural and biological control of Canada thistle in conservation tillage soybean. Weed Sci 49:642–646.Google Scholar
Jennings, J. C., Apel-Birkhold, P. C., Bailey, B. A., and Anderson, J. D. 2000. Induction of ethylene biosynthesis and necrosis in weed leaves by a Fusarium oxysporum protein. Weed Sci 48:7–14.Google Scholar
Jennings, J. C., Apel-Birkhold, P. C., Mock, N. M., Baker, C. J., Anderson, J. D., and Bailey, B. A. 2001. Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum . Plant Sci 161:891–899.Google Scholar
Johnson, D. R., Wyse, D. L., and Jones, K. J. 1996. Controlling weeds with phytopathogenic bacteria. Weed Technol 10:621–624.Google Scholar
Kado, C. I. and Heskett, M. G. 1970. Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas . Phytopathology 60:969–976.CrossRefGoogle ScholarPubMed
Keates, S. E., Kostman, T. A., Anderson, J. D., and Bailey, B. A. 2003. Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis. Plant Physiol. In press.Google Scholar
Koch, W., Wagner, C., and Seitz, H. U. 1998. Elicitor-induced cell death and phytoalexin synthesis in Daucus carota . L. Planta 206:523–532.Google Scholar
Lukens, J. H. and Durbin, R. D. 1985. Tagetitoxin affects plastid development in seedlings of wheat. Planta 165:311–321.Google Scholar
Mathews, D. E. and Durbin, R. D. 1990. Tagetitoxin inhibits RNA synthesis directed by RNA polymerases from chloroplasts and Escherichia coli . J. Biol. Chem 265:493–498.Google Scholar
Mathews, D. E. and Durbin, R. D. 1994. Mechanistic aspects of tagetitoxin inhibition of RNA polymerase from Escherichia coli . Biochemistry 33:11987–11992.Google Scholar
Rhodehamel, N. H. and Durbin, R. D. 1985. Host range of strains of Pseudomonas syringae pv. tagetis . Plant Dis 69:589–591.Google Scholar
Steinberg, T. H., Mathews, D. E., Durbin, R. D., and Burgess, R. R. 1990. Tagetitoxin: a new inhibitor of eukaryotic transcription by RNA polymerase III. J. Biol. Chem 265:499–505.Google Scholar
Styer, D. J. and Durbin, R. D. 1982. Common ragweed: a new host for Pseudomonas syringae pv. tagetis . Plant Dis 66:71.Google Scholar
Veit, S., Worle, J. M., Nurnberger, T., Koch, W., and Seitz, H. U. 2001. A novel protein elicitor (PaNie) from Pythium aphanidermatum induces multiple defense responses in carrot, Arabidopsis, and tobacco. Plant Physiol 127:832–841.Google Scholar