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Effects of Haloxyfop and CGA-82725 on Cell Cycle and Cell Division of Oat (Avena sativa) Root Tips

Published online by Cambridge University Press:  12 June 2017

Jae C. Kim  and
Leo E. Bendixen
Jae C. Kim
Affiliation:
Dep. Agron., Ohio State Univ., Columbus, OH 43210
Leo E. Bendixen
Affiliation:
Dep. Agron., Ohio State Univ., Columbus, OH 43210
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Abstract

The mode of action of two experimental pyridinyloxyphenoxy propionate herbicides, haloxyfop {2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl]oxy] phenoxy] propanoic acid} and CGA-82725 {2-propynyl-2-[4-[(3,5-dichloro-2-pyridinyl)oxy] phenoxy] propanoic acid} was studied. Oat (Avena sativa L.) roots showed a rate-dependent sensitivity to both herbicides. Consequently, a series of assays was performed to study cell division, cell cycle dynamics, and nucleic acid and protein synthesis in oat root tips in order to characterize growth inhibition. Both herbicides inhibited cell division, apparently by inhibiting protein synthesis in the G2 stage of interphase. Supporting evidence for this assumption comes from the following observations: a) There was almost complete absence of leucine incorporation within 1 h of exposure to 2.7 × 10−4 M of either herbicide; b) the number of 3H-labeled dividing cells was reduced to about 20% of the control within 4 h, and essentially to zero within 8 h, after exposure to 2.7 × 10−4 M of herbicide; c) the number of unlabeled cells was reduced to less than 10% of the control within 8 h of exposure to 2.7 × 10−4 M of herbicide; d) during this 8-h period, the number of 3H-labeled cells in interphase which had been exposed to herbicide greatly exceeded the control; e) thymidine incorporation into DNA was inhibited but was not proportional to reduction in cell division; f) uridine incorporation was enhanced during the first 8 h of exposure before a reduction was evident at 12 h; and g) an oat coleoptile elongation bioassay showed that cell enlargement was not inhibited at herbicide concentrations less than 2.7 × 10−4 M.

Keywords

DNA synthesisRNA synthesisprotein synthesisleucine incorporationthymidine incorporationuridine incorporation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 35 , Issue 6 , November 1987 , pp. 769 - 774
Copyright
Copyright © 1987 by the Weed Science Society of America 

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References

Literature Cited

1. Bendixen, L. E. 1987. Soybean (Glycine max) competition helps herbicides control johnsongrass (Sorghum halepense). Weed Technol. (In press).CrossRefGoogle Scholar
2. Buhler, D. D., Swisher, B. A., and Burnside, O. C. 1985. Behavior of 14C-haloxyfop-methyl in intact plants and cell cultures. Weed Sci. 33:291–299.CrossRefGoogle Scholar
3. Cho, H. Y., Widholm, J. M., and Slife, F. W. 1986. Effects of haloxyfop on corn (Zea mays) and soybean (Glycine max) cell suspension cultures. Weed Sci. 34:496–501.CrossRefGoogle Scholar
4. Deal, L. M. and Hess, F. D. 1980. An analysis of the growth inhibitory characteristics of alachlor and metolachlor. Weed Sci. 28:168–175.CrossRefGoogle Scholar
5. Gimenez-Martin, G., de la Torre, C., and Lopez-Saez, J. F. 1977. Cell division in higher plants. Pages 261–307 in Rost, T. L. and Gifford, E. M. Jr. Mechanisms and control of cell division. Dowden, Hutchinson & Ross, Inc., Stroudsburg, PA.Google Scholar
6. Gronwald, J. W. 1986. Effect of haloxyfop and haloxyfop-methyl on elongation and respiration of corn (Zea mays) and soybean (Glycine max) roots. Weed Sci. 34:196–202.CrossRefGoogle Scholar
7. Harkes, P.P.A. 1973. Structure and dynamics of the root cap of Avena sativa L. Acta Bot. Neerl. 22(4): 321–328.CrossRefGoogle Scholar
8. Hendley, P., Dicks, J. W., Monaco, T. J., Slyfield, S. M., Tummon, O. J., and Barrett, J. C. 1985. Translocation and metabolism of pyridinyloxyphenoxypropionate herbicides in rhizomatous quackgrass (Agropyron repens). Weed Sci. 33:11–24.CrossRefGoogle Scholar
9. Peregoy, R. S. and Glenn, S. 1985. Physiological responses to fluazifop-butyl in tissue of corn (Zea mays) and soybean (Glycine max). Weed Sci. 33:443–446.CrossRefGoogle Scholar
10. Ray, T. B. 1982. The mode of action of chlorsulfuron: A new herbicide for cereals. Pestic. Biochem. Physiol. 17:10–17.CrossRefGoogle Scholar
11. Rost, T. L. and Bayer, D. E. 1976. Cell cycle population kinetics of pea root tip meristems treated with propham. Weed Sci. 24: 81–87.CrossRefGoogle Scholar
12. Setterfield, G., Schreiber, R., and Woodard, J. 1954. Mitotic frequency determinations and microphotometric feulgen dye measurements in root tips. Stain Technol. 29(3): 113–120.CrossRefGoogle ScholarPubMed
13. Hof, J. Van't 1966. Experimental control of DNA synthesizing and dividing cells in excised root tips of Pisum . Am. J. Bot. 53:970–976.CrossRefGoogle Scholar
14. Hof, J. Van't 1968. Experimental procedure for measuring cell population kinetics parameters in plant root meristems. Pages 195–217 in D. Prescott Methods in Cell Physiology V. 3rd ed. Google Scholar
15. Hof, J. Van't and Kovacs, C. J. 1972. Mitotic cycle regulation in the meristem of cultured roots: The principal control point hypothesis. Adv. Exp. Med. Biol. 18:15–32.Google Scholar
16. Webster, P. L. and Hof, J. Van't 1970. DNA synthesis and mitosis in meristems: Requirements for RNA and protein synthesis. Am. J. Bot. 57:130–139.Google Scholar