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Effects of Clomazone on IPP Isomerase and Prenyl Transferase Activities in Cell Suspension Cultures and Cotyledons of Solanaceous Species

Published online by Cambridge University Press:  12 June 2017

Jon E. Scott ,
Leslie A. Weston ,
Joseph Chappell  and
Kathleen Hanley
Jon E. Scott
Affiliation:
Dep. of Hortic. and Landscape Arch., Univ. Kentucky, Lexington, KY 40546
Leslie A. Weston
Affiliation:
Dep. of Agron., Univ. Kentucky, Lexington, KY 40546
Joseph Chappell
Affiliation:
Dep. of Agron., Univ. Kentucky, Lexington, KY 40546
Kathleen Hanley
Affiliation:
Biosource Genetics Co., Bowman Gray Tech. Ctr., Bldg. 611, Winston Salem, NC 27102
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Abstract

Laboratory assays were conducted to determine the sensitivity of tomato and tobacco cell suspension cultures and tomato and pepper cotyledons to clomazone. A comparison of fresh weight and carotenoid content indicated up to a three-fold difference between the clomazone-tolerant tobacco and clomazone-susceptible tomato cell suspension cultures. In contrast, an approximate 60-fold difference between the tolerant pepper and susceptible tomato cotyledons was observed when total chlorophyll and carotenoid contents were measured. The effect of clomazone and its possible metabolites on in vivo and in vitro extractable IPP isomerase (EC 5.3.3.2) and prenyltransferase (EC 2.5.1.29) activity was investigated. There was no clear inhibitory effect of clomazone or possible clomazone metabolites upon enzyme activity in tomato or tobacco cell suspension cultures or on light or dark grown tomato or pepper cotyledons. No specific enzymatic target site of clomazone was identified in correlation with the reduction in total chlorophyll or carotenoid content.

Keywords

Clomazone, 2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinonepepper, Capsicum annum L. cv Yolo Wonder L.tobacco, Nicotiana tabacum L. ‘KY-14’tomato, Lycopersicon esculentum Mill. ‘Niagara.’CarotenoidschlorophylldimethazoneFMC 57020selectivity
Type
Physiology, Chemistry, and Biochemostry
Information
Weed Science , Volume 42 , Issue 4 , December 1994 , pp. 509 - 516
Copyright
Copyright © 1994 by the Weed Science Society of America 

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