Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-09T20:27:45.007Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of rust-causing pathogen (Puccinia thlaspeos) on auxin-like and cytokinin-like activity in dyer's woad (Isatis tinctoria)

Published online by Cambridge University Press:  20 January 2017

Wendy A. Stirk ,
Sherman V. Thomson  and
Johannes van Staden
Sherman V. Thomson
Affiliation:
Department of Biology, Utah State University, Logan, UT 84322-5305
Johannes van Staden
Affiliation:
Research Centre for Plant Growth and Development, University of KwaZulu-Natal Pietermaritzburg, P/Bag X01 Scottsville 3209, South Africa
Get access Rights & Permissions [Opens in a new window]

Abstract

The rust pathogen, Puccinia thlaspeos, is used for biocontrol of dyer's woad. Rust infection results in chlorotic leaves and abnormal flower and seed production of this noxious weed during its second year of growth. Auxin-like and cytokinin-like activity of healthy and infected plants (roots, shoots, and leaves) were determined during different growth stages. These samples were analyzed by use of the mung-bean rooting bioassay (auxin-like activity) and the soybean-callus bioassay (cytokinin-like activity). Rooting activity in the mung-bean bioassay was significantly higher in the infected-root extracts from Harvest 1 (rosette stage) compared with the healthy-root extracts and significantly higher in the healthy-shoot extracts from Harvest 2 (bolting plants) compared with infected-shoot extracts. Infected plants collected during Harvest 1 had greatly reduced cytokinin-like activity in the roots, shoots, and leaves compared with the healthy plants. Cytokinin-like activity increased slightly in the infected plants collected in subsequent harvests, with the highest activity recorded in root extracts. The results suggest potential ways that the rust-causing fungal pathogen is able to influence some physiologic processes in dyer's woad and so affect its growth.

Keywords

Dyer's woad, Isatis tinctoria L., ISCRUrust, Puccinia thlaspeos C. Shubmung bean, Vigno mungo L.soybean, Glycine max (L.) MerrFloweringmung-bean bioassaysoybean-callus bioassay
Type
Research Article
Information
Weed Science , Volume 54 , Issue 5 , October 2006 , pp. 815 - 820
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Banowetz, G. M. 1997. Cultivars of hexaploid wheat of contrasting stature and chlorophyll retention differ in cytokinin content and responsiveness. Ann. Bot. 79:185–190.CrossRefGoogle Scholar
Callihan, R. H., Dewy, S. A., Patton, J. E., and Thill, D. C. 1984. Distribution, biology and habitat of dyers woad (Isatis tinctoria L.) in Idaho. J. Idaho Acad. Sci. 20:18–31.Google Scholar
Crouch, I. J. and van Staden, J. 1991. Evidence for rooting factors in a seaweed concentrate prepared from Ecklonia maxima . J. Plant Physiol. 137:319–322.CrossRefGoogle Scholar
Daly, J. M. and Deverall, B. J. 1963. Metabolism of indoleacetic acid in rust diseases. I. Factors influencing rates of decarboxylation. Plant Physiol. 38:741–750.CrossRefGoogle Scholar
Dobrev, P. I. and Kamínek, M. 2002. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. J. Chromat. A. 950:21–29.CrossRefGoogle ScholarPubMed
Evans, J. O. and Chase, R. L. 1981. Dyers woad control. Utah State Univ. Extension Bull. EL:199.Google Scholar
Farah, K. O., Tanaca, A. F., and West, N. F. 1988. Autoecology and population biology of dyers woad (Isatis tinctoria). Weed Sci. 36:186–193.CrossRefGoogle Scholar
Flint, K. M. and Thomson, S. V. 2000. Seasonal infection of the weed dyer's woad by a Puccinia sp. rust used for biocontrol, and effects of temperature on basidiospore production. Plant Dis. 84:753–759.CrossRefGoogle ScholarPubMed
Greene, E. M. 1980. Cytokinin production by microorganisms. Bot. Rev. 46:25–74.CrossRefGoogle Scholar
Gruen, H. E. 1959. Auxins and fungi. Annu. Rev. Plant Physiol. 10:405–440.CrossRefGoogle Scholar
Horgan, R. 1984. Cytokinins. Pages 53–75 in Wilkins, M. B. ed. Advanced Plant Physiology. Essex, UK: Longman.Google Scholar
Kamínek, M. 1992. Progress in cytokinin research. Trends Biotechnol. 10:159–164.CrossRefGoogle Scholar
Kropp, B. R., Albee, S., Flint, K. M., Zambino, P., Szabo, L., and Thomson, S. V. 1995. Early detection of systemic rust infections of dyers woad (Isatis tinctoria) using the polymerase chain reaction. Weed Sci. 43:467–472.CrossRefGoogle Scholar
Miller, C. O. 1965. Evidence for the natural occurrence of zeatin and derivatives: compounds from maize which promote cell division. Proc. Natl. Acad. Sci. U.S.A. 54:1052–1058.CrossRefGoogle ScholarPubMed
Nordström, A., Tarkowski, P., Tarkowska, D., Norbaek, R., Åstot, C., Dolezal, K., and Sandberg, G. 2004. Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development. Proc. Natl. Acad. Sci. U.S.A. 101:8039–8044.CrossRefGoogle ScholarPubMed
Normanly, J. 1997. Auxin metabolism. Physiol. Plant. 100:431–442.CrossRefGoogle Scholar
Srivastava, H. S., Ormrod, D. P., and Hale, B. A. 1994. Cytokinins affect the responses of greening and green bean leaves to nitrogen dioxide and nutrient nitrate supply. J. Plant Physiol. 144:156–160.CrossRefGoogle Scholar
Taylor, N. J. and van Staden, J. 2006. Towards an understanding of the manipulation of in vitro flowering. Pages 1–22 in Teixeira da Silva, J. A. ed. Floriculture, Ornamental and Plant Biotechnology, vol. 3. London, UK: Global Science Books.Google Scholar
Young, J. A. and Evans, R. A. 1971. Germination of dyers woad. Weed Sci. 19:76–78.CrossRefGoogle Scholar