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Detecting Annual Bluegrass (Poa annua) Resistance to ALS-Inhibiting Herbicides Using a Rapid Diagnostic Assay

Published online by Cambridge University Press:  20 January 2017

Robert B. Cross ,
Lambert B. McCarty ,
Nishanth Tharayil ,
Ted Whitwell  and
William C. Bridges Jr.
Robert B. Cross*
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, Clemson University, Clemson, SC 29634
Lambert B. McCarty
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, Clemson University, Clemson, SC 29634
Nishanth Tharayil
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, Clemson University, Clemson, SC 29634
Ted Whitwell
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, Clemson University, Clemson, SC 29634
William C. Bridges Jr.
Affiliation:
Department of Mathematical Sciences, Clemson University, Clemson, SC 29634
*
Corresponding author's E-mail: [email protected]
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Abstract

Annual bluegrass is the most problematic winter annual weed in managed turfgrass. Acetolactate synthase (ALS)-inhibiting herbicides are effective for annual bluegrass control, but reliance on this mode of action can select for herbicide-resistant biotypes. Two annual bluegrass biotypes not controlled with ALS-inhibiting herbicides were reported at golf courses in South Carolina and Georgia. Research was initiated at Clemson University to verify the level of resistance of these biotypes to ALS inhibitors. Two ALS-susceptible (S) and suspected resistant (SCr, GAr) annual bluegrass biotypes were established in a greenhouse. Dose-response experiments were conducted on mature annual bluegrass plants using trifloxysulfuron, foramsulfuron, and bispyribac-sodium, all ALS-inhibiting herbicides. Additionally, a rapid diagnostic ALS activity assay was optimized and conducted using the same herbicides. For dose-response experiments, the rate of herbicide that reduced shoot biomass 50% (I50) values for the S biotypes were 13.6 g ai ha−1 for trifloxysulfuron, 7.0 g ai ha−1 for foramsulfuron, and 38.3 g ai ha−1 for bispyribac-sodium. Fifty percent shoot biomass reduction was not observed in either the SCr or GAr biotypes at eight times the labeled field rate of all ALS-inhibiting herbicides tested. For in vivo tests of ALS activity, the SCr biotype yielded I50 (concentration of herbicide that reduced ALS activity 50%) values 3,650, 3,290, and 13 times the S biotypes following treatment with trifloxysulfuron, foramsulfuron, and bispyribac-sodium, respectively. Similarly, I50 values for the GAr biotype were 316, 140, and 64 times greater than the S biotypes following the same herbicide treatments. This research indicates high levels of annual bluegrass resistance to multiple ALS-inhibiting herbicides in South Carolina and Georgia. Future research should focus on the mechanisms of ALS resistance in these annual bluegrass biotypes as well as alternative options for control not targeting the ALS enzyme.

Keywords

Bispyribac-sodiumforamsulfurontrifloxysulfuronannual bluegrass, Poa annua LBermudagrassCynodon dactylon (L.) Persgolf courseherbicide resistanceturfgrass
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 3 , September 2013 , pp. 384 - 389
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Anonymous, . 2007. Revolver herbicide label. Research Triangle Park, NC Bayer Environmental Science. Publication No. 061017C. 10 p.Google Scholar
Anonymous, . 2009. Monument 75 WG herbicide label. Greensboro, NC Syngenta Crop Protection, Inc. Publication No. SCP 1134A-L1C 0909 302209. 24 p.Google Scholar
Anonymous, . 2010. Velocity SG herbicide label. Walnut Creek, CA Valent U.S.A. Corporation. Publication No. 2010-Vel-0001. 10 p.Google Scholar
Beard, J. B. 1970. An ecological study of annual bluegrass. U. S. Golf Assoc. Green Sect. Rec. 8:13–18.Google Scholar
Beard, J. B., Rieke, P. E., Turgeon, A. J., and Vargas, J. M. 1978. Annual bluegrass (Poa annua L.) description, adaptation, culture, and control. Research Report 352. Michigan State University Agricultural Experiment Station. East Lansing, MI., 32 p.Google Scholar
Brosnan, J. T., Breeden, G. K., and Mueller, T. C. 2012. A glyphosate-resistant biotype of annual bluegrass in Tennessee. Weed Sci. 60:97–100.Google Scholar
Christopher, J. T., Powles, S. B., and Holtum, J.A.M. 1992. Resistance to acetolactate synthase–inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol. 100:1909–1913.Google Scholar
Duggleby, R. G., McCourt, J. A., and Guddat, L. W. 2008. Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol. Biochem. 46:309–324.Google Scholar
Duggleby, R. G., Pang, S. S., Yu, H., and Guddat, L. W. 2003. Systematic characterization of mutations in yeast acetohydroxyacid synthase: interpretation of herbicide-resistance data. Eur. J. Biochem. 270:2895–2904.Google Scholar
Gerwick, C. B., Mireles, L. C., and Eilers, R. J. 1993. Rapid diagnosis of ALS/AHAS-resistant weeds. Weed Technol. 7:519–524.Google Scholar
Heap, I. 2013. The International Survey of Herbicide Resistant Weeds. www.weedscience.org. Accessed January 24, 2013.Google Scholar
Isgrigg, J. III, Yelverton, F. H., Brownie, C., and Warren, L. S. Jr. 2002. Dinitroaniline resistant annual bluegrass in North Carolina. Weed Sci. 50:86–90.Google Scholar
Kelly, S. T., Coats, G. E., and Luthe, D. S. 1999. Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technol. 13:747–752.CrossRefGoogle Scholar
Kuk, Y. I., Jung, H. I., Kwon, O. D., Lee, D. J., Burgos, N. R., and Guh, J. O. 2003. Rapid diagnosis of resistance to sulfonylurea herbicides in monochoria (Monochoria vaginalis). Weed Sci. 51:305–311.Google Scholar
Lush, W. M. 1989. Adaptation and differentiation of golf course populations of annual bluegrass (Poa annua). Weed Sci. 37:54–59.Google Scholar
Mallory-Smith, C. A., Thill, D. C., and Dial, M. J. 1990. Identification of sulfonylurea herbicide–resistant prickly lettuce (Lactuca serriola). Weed Tech. 4:163–168.Google Scholar
Mao, Q. and Huff, D. R. 2012. The evolutionary origin of Poa annua L. Crop Sci. 52:1910–1922.Google Scholar
McCarty, L. B. 2011. Turfgrass weeds. Pp. 586–620 in McCarty, L. B., ed. Best Golf Course Management Practices. 3rd ed. Upper Saddle River, NJ Prentice Hall.Google Scholar
McElroy, J. S., Breeden, G. K., and Wehtje, G. 2011. Evaluation of annual bluegrass control programs for bermudagrass turf overseeded with perennial ryegrass. Weed Technol. 25:58–63.Google Scholar
McElroy, J. S., Flessner, M. L., Wang, Z., Dane, F., Walker, R. H., and Wehtje, G. R. 2013. A Trp574 to Leu amino acid substitution in the ALS gene of annual bluegrass (Poa annua) is associated with resistance to ALS-inhibiting herbicides. Weed Sci. 61:21–25.Google Scholar
Primiani, M. M., Cotterman, J. C., and Saari, L. L. 1990. Resistance of kochia (Kochia scoparia) to sulfonylurea and imidazolinone herbicides. Weed Technol. 4:169–172.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218–227.CrossRefGoogle Scholar
Shaner, D. L. 1991. Physiological effects of the imidazolinone herbicides. Pp. 129–138 in Shaner, D. L., and O'Connor, S. L., eds. The Imidazolinone Herbicides. Ann Arbor, MI Lewis.Google Scholar
Toler, J. E., Willis, T. G., Estes, A. G., and McCarty, L. B. 2007. Postemergent annual bluegrass control in dormant nonoverseeded bermudagrass turf. HortScience 42:670–672.Google Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700–712.Google Scholar
Uchino, A., Watanabe, H., Wang, G., and Itoh, K. 1999. Light requirement in rapid diagnosis of sulfonylurea-resistant weeds of Lindernia spp. (Scrophulariaceae). Weed Technol. 13:680–684.Google Scholar
Umbarger, H. E. 1978. Amino acid biosynthesis and its regulation. Annu. Rev. Bioch. 47:533–606.CrossRefGoogle ScholarPubMed
Westerfeld, W. W. 1945. A colorimetric determination of blood acetoin. J. Biol. Chem. 161:495–502.Google Scholar
Yu, Q., Han, H., Vila-Aiub, M. M., and Powles, S. B. 2010. AHAS herbicide resistance endowing mutations: effect on AHAS functionality and plant growth. J. Exp. Bot. 61:3925–3934.Google Scholar