Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-22T16:05:25.787Z Has data issue: false hasContentIssue false
  • English
  • Français

Cowpea (Vigna unguiculata) Seed Protein Response to Glyphosate

Published online by Cambridge University Press:  12 June 2017

Antonio L. Cerdeira ,
A. Wayne Cole  and
Dawn S. Luthe
Antonio L. Cerdeira
Affiliation:
Dep. of Plant Pathol. and Weed Sci.
A. Wayne Cole
Affiliation:
Dep. of Plant Pathol. and Weed Sci.
Dawn S. Luthe
Affiliation:
Dep. of Biochem., Mississippi State Univ., Mississippi State, MS 39762
Get access Rights & Permissions [Opens in a new window]

Abstract

Electrophoretic analysis of storage protein accumulation in developing cowpea [Vigna unguiculata (L.) Walp. ‘Mississippi Purple’] seed indicated that a large increase in protein content per seed occurred between 10 and 11 days after flowering (DAF). Polypeptides with molecular weights of 54, 49, and 41 kilodaltons (kD) accumulated first, and one with a molecular weight of 59 kD appeared 2 days later at 13 DAF. Treatment of plants with glyphosate [N-(phosphonomethyl)glycine] when pods were 7 and 10 DAF prevented accumulation of the major storage protein polypeptides. The accumulation of these polypeptides was not inhibited as much when pods were 11, 12, or 13 DAF at the time of treatment with glyphosate. Pod length and seed fresh weight were inhibited by glyphosate treatment of plants bearing pods 7, 10, 11, and 12 DAF. Pod width, seed dry weight, and seed length were inhibited by glyphosate when plants bearing pods 7, 10, and 11 DAF were treated.

Keywords

Electrophoresispolypeptidesseed weight
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 33 , Issue 1 , January 1985 , pp. 1 - 6
Copyright
Copyright © 1985 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Amrhein, N., Schab, J., and Steinrucken, H.C. 1980. The mode of action of the herbicide glyphosate. Naturwissenschaften 67:356–357.CrossRefGoogle Scholar
2. Bovey, R. W. 1975. Preharvest desiccation of grain sorghum with glyphosate. Agron. J. 67:618–621.Google Scholar
3. Carasco, V. F., Croy, R., Derbyshire, E., and Boulter, D. 1978. The isolation and characterization of the major polypeptides of the seed globulin of cowpea (Vigna unquiculata L. Walp.) and their sequential synthesis in developing seeds. J. Exp. Bot. 29:309–323.Google Scholar
4. Cole, A. W. and Cerdeira, A. L. 1982. Southernpea response to glyphosate desiccation. HortScience 17(2):244–246.CrossRefGoogle Scholar
5. Islam Kahn, M. R., Gatehouse, J. A., and Boulter, D. 1980. The seed proteins of cowpea (Vigna unquiculata L. Walp.). J. Exp. Bot. 31:1599–1611.Google Scholar
6. Klingman, D. L. and Murray, J. J. 1976. Germination of seeds of turfgrasses as affected by glyphosate and paraquat. Weed Sci. 24:191–193.Google Scholar
7. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685.Google Scholar
8. Murray, D. R., MacKenzie, K. F., Vairinhos, F., Peoples, M. B., Atkins, C. A., and Pate, J. S. 1983. Electrophoretic studies of the seed protein of cowpea, Vigna unquiculata (L.) Walp. Z. Pflanzenphysiol. 109:363–370.Google Scholar
9. Steinrucken, H. C. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic-acid-3-phosphate synthase. Biochem. Biophys. Res. Comm. 94:1207–1212.CrossRefGoogle ScholarPubMed
10. Weed Science Society of America. 1983. Herbicide Handbook. Weed Science Society of America, Champaign, IL. 515 pp.Google Scholar