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Comparative Detoxification of Chlorsulfuron in Leaf Disks and Cell Cultures of Two Perennial Weeds

Published online by Cambridge University Press:  12 June 2017

Beth A. Swisher  and
Monte R. Weimer
Beth A. Swisher
Affiliation:
Dep. Agron., Univ. Nebraska, Lincoln, NE 68583
Monte R. Weimer
Affiliation:
Dep. Agron., Univ. Nebraska, Lincoln, NE 68583
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Abstract

Phytotoxicity of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino] carbonyl] benzenesulfonamide} in cell cultures of Canada thistle [Cirsium arvense (L.) Scop. # CIRAR] was approximately 30-fold greater than in leafy spurge (Euphorbia esula L. # EPHES) cultures. Differences in chlorsulfuron phytotoxicity in these two species were attributed to large differences in cellular metabolism of the herbicide. Leafy spurge cells metabolized all of the applied 14C-chlorsulfuron within 72 h of treatment, while Canada thistle metabolized less than 2%. Acid hydrolysis of the metabolic products isolated from leaf disks and cell cultures of leafy spurge yielded a radioactive product that cochromatographed with a 2-chlorobenzenesulfonamide standard. Therefore, metabolic transformation occurred on the heterocyclic portion of the molecule. A major metabolite was further characterized as 2-chloro-N-[[4-(hydroxymethyl)-6-methoxy-1,3,5-triazin-2-yl]aminocarbonyl]-benzenesulfonamide by cochromatography with the authentic standard.

Keywords

Herbicide detoxificationherbicide metabolismpesticide metabolismCirsium arvenseEuphorbia esulaCIRAREPHES
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 34 , Issue 4 , July 1986 , pp. 507 - 512
Copyright
Copyright © 1986 by the Weed Science Society of America 

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