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Characterization of the Orobanche–Arabidopsis system for studying parasite–host interactions

Published online by Cambridge University Press:  20 January 2017

James H. Westwood
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Abstract

Parasitization by Orobanche is a complex process, one that is mediated by host-derived chemical signals that control parasite seed germination and haustorium initiation and one that ultimately results in the union of two plant species. Experiments were conducted to characterize Orobanche parasitization of the model plant Arabidopsis thaliana and to begin to explore the role of host flavonoid metabolism in the interaction. Arabidopsis thaliana stimulated seed germination and allowed tubercle development of O. aegyptiaca and O. ramosa but did not significantly stimulate seeds of O. crenata, O. minor, or O. cernua. However, if Orobanche seeds were artificially stimulated, O. crenata and O. minor successfully established tubercles on A. thaliana. When compared to the recognized crop hosts, Daucus carota and Nicotiana tabacum, A. thaliana stimulated less O. aegyptiaca germination but allowed for formation of equivalent numbers of tubercles. These findings indicate that A. thaliana is not a large-scale producer of germination stimulant but is highly susceptible to the parasite once Orobanche seeds have germinated. Experiments comparing wild-type A. thaliana plants to mutant lines deficient in flavonoid biosynthesis revealed no differences in the ability to stimulate germination or to allow tubercle formation, indicating that host flavonoid production is not essential for Orobanche parasitization. The results of this work support the use of A. thaliana as a valuable host in understanding Orobanche parasitization.

Keywords

Arabidopsis thaliana L. Heynh., ARBTH, mouseearcressDaucus carota L. ‘Danver half long’, DAUCS, carrotNicotiana tabacum L. ‘Coker 319’, NIOTA, tobaccoOrobanche aegyptiaca Pers., ORAAE, Egyptian broomrapeOrobanche cernua Loefl., ORACE, nodding broomrapeOrobanche crenata Forsk., ORACR, crenate broomrapeOrobanche minor Smith, ORAMI, small broomrapeOrobanche ramosa L., ORARA, branched broomrapeParasitismhost specificityflavonoid mutantssusceptibility
Type
Research Article
Information
Weed Science , Volume 48 , Issue 6 , December 2000 , pp. 742 - 748
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Albrecht, H., Yoder, J. I., and Phillips, D. A. 1999. Flavonoids promote haustoria formation in the root parasite Triphysaria versicolor . Plant Physiol. 119:585–591.Google Scholar
Alonso, L. C. 1998. Resistance to Orobanche and resistance breeding: a review. Pages 233–257 In Wegmann, K., Musselman, L. J., and Joel, D. M., eds. Proceedings of the Fourth International Workshop on Orobanche . Albena, Bulgaria: Institute for Wheat and Sunflower “Dobroudja.”Google Scholar
Burbulis, I. E., Iacobucci, M., and Shirley, B. W. 1996. A null mutation in the first enzyme of flavonoid biosynthesis does not affect male fertility in Arabidopsis . Plant Cell 8:1013–1025.Google Scholar
Chang, M., Netzly, D. H., Butler, L. G., and Lynn, D. G. 1986. Chemical regulation of distance: characterization of the first natural host germination stimulant for Striga asiatica . J. Am. Chem. Soc. 108:7858–7860.CrossRefGoogle ScholarPubMed
Cook, C. E., Whichard, L. P., Turner, B., Wall, M. E., and Egley, G. H. 1966. Germination of witchweed (Striga lutea Lour.): isolation and properties of a potent stimulant. Science 154:1189–1190.CrossRefGoogle ScholarPubMed
Cook, C. E., Whichard, L. P., Wall, M. E., Egley, G. H., Coggon, P., Luhan, P. A., and McPhail, A. T. 1972. Germination stimulants. II. The structure of strigol—a potent seed germination stimulant for witchweed (Striga lutea Lour.). J. Am. Chem. Soc. 94:6198–6199.CrossRefGoogle Scholar
Cubero, J. I. 1991. Breeding for resistance to Orobanche species: a review. Pages 257–277 In Wegmann, K. and Musselman, L. J., eds. Progress in Orobanche Research. Tübingen, FRG: Eberhard-Karls-Universität.Google Scholar
Davies, K. R. and Hammerschmidt, R., eds. 1993. Arabidopsis thaliana as a Model for Plant-Pathogen Interactions. St. Paul, MN: APS Press. 134 p.Google Scholar
Estabrook, E. M. and Yoder, J. I. 1998. Plant-plant communications: rhizosphere signaling between parasitic angiosperms and their hosts. Plant Physiol. 116:1–7.Google Scholar
Foy, C. L., Jain, R., and Jacobsohn, R. 1989. Recent approaches for chemical control of broomrape (Orobanche spp.). Rev. Weed Sci. 4:123–152.Google Scholar
Goldwasser, Y., Kleifeld, Y., and Rubin, B. 1997. Variation in vetch (Vicia spp.) response to Orobanche aegyptiaca . Weed Sci. 45:756–762.CrossRefGoogle Scholar
Goldwasser, Y., Plakhine, D., and Yoder, J. L. 2000. Arabidopsis thaliana susceptibility to Orobanche spp. Weed Sci. 48:342–346.CrossRefGoogle Scholar
Graves, J. D. 1995. Host-plant responses to parasitism. Pages 206–225 In Press, M. C. and Graves, J. D., eds. Parasitic Plants. London: Chapman & Hall.Google Scholar
Hauck, C., Müller, S., and Schildknecht, H. 1992. A germination stimulant for parasitic flowering plants from Sorghum bicolor, a genuine host plant. J. Plant Physiol. 139:474–478.Google Scholar
Hoagland, D. R. and Arnon, D. I. 1950. The water-culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ. 347:1–32.Google Scholar
Joel, D. M. 1998. The angiospermous root parasite Orobanche L. (Orobanchaceae) induces expression of a pathogenesis related (PR) gene in susceptible tobacco roots. Ann. Bot. 81:779–781.Google Scholar
Joel, D. M. and Losner-Goshen, D. 1994. The attachment organ of the parasitic angiosperms Orobanche cumana and O. aegyptiaca and its development. Can. J. Bot. 72:564–574.Google Scholar
Lynn, D. G. and Chang, M. 1990. Phenolic signals in cohabitation: implications for plant development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41:497–526.CrossRefGoogle Scholar
Mangnus, E. M., Stommen, P.L.A., and Zwanenburg, B. 1992. A standardized bioassay for evaluation of potential germination stimulants for seeds of parasitic weeds. J. Plant Growth Regul. 11:91–98.CrossRefGoogle Scholar
Müller, S., Hauck, C., and Schildknecht, H. 1992. Germination stimulants produced by Vigna unguiculata Walp. cv Saunders Upright. J. Plant Growth Regul. 11:77–84.Google Scholar
Meinke, D. W., Cherry, J. M., Dean, C., Rounsley, S. D., and Koornneef, M. 1998. Arabidopsis thaliana: a model plant for genome analysis. Science 282:662–682.Google Scholar
Parker, C. and Riches, C. R. 1993. Parasitic Weeds of the World: Biology and Control. Wallingford, United Kingdom: CAB International.Google Scholar
Press, M. C., Graves, J. D., and Stewart, G. R. 1990. Physiology of the interaction of angiosperm parasites and their higher plant hosts. Plant Cell Environ. 13:91–104.Google Scholar
Sauerborn, J. 1991. The economic importance of the phytoparasites Orobanche and Striga . Pages 137–143 In Ransom, J. K., Musselman, L. J., Worsham, A. D., and Parker, C., eds. Proceedings of the 5th International Symposium of Parasitic Weeds. Nairobi, Kenya: CIMMYT.Google Scholar
Scheres, B. and Wolkenfelt, H. 1998. The Arabidopsis root as a model to study plant development. Plant Physiol. Biochem. 36:21–32.CrossRefGoogle Scholar
Shirley, B. W., Kubasek, W. L., Storz, G., Bruggemann, E., Koornneef, M., Ausubel, F. M., and Goodman, H. M. 1995. Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis. Plant J. 8:659–671.CrossRefGoogle ScholarPubMed
Smith, C. E., Dudley, M. W., and Lynn, D. G. 1990. Vegetative/parasitic transition: control and plasticity in Striga development. Plant Physiol. 93:208–215.Google Scholar
Stewart, G. R. and Press, M. C. 1990. The physiology and biochemistry of parasitic angiosperms. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41:127–151.CrossRefGoogle Scholar
Timko, M. P., Florea, C. S., and Riopel, J. L. 1989. Control of germination and early development in parasitic angiosperms. Pages 225–240 In Taylorson, R. B., ed. Recent Advances in the Development and Germination of Seeds. New York: Plenum.CrossRefGoogle Scholar
Westwood, J. H. and Foy, C. L. 1998. Arabidopsis thaliana can be a model host for Orobanche research. Pages 155–160 In Wegmann, K., Musselman, L. J., and Joel, D. M., eds. Current Problems of Orobanche Research, Proceedings of the Fourth International Workshop on Orobanche . Albena, Bulgaria: Institute for Wheat and Sunflower “Dobroudja.”Google Scholar
Westwood, J. H., Yu, X., Foy, C. L., and Cramer, C. L. 1998. Expression of a defense-related 3-hydroxy-3-methylglutaryl CoA reductase gene in response to parasitization by Orobanche spp. Mol. Plant-Microbe Interact 11:530–536.Google Scholar
Yoder, J. I. 1999. Parasitic plant responses to host plant signals: a model for subterranean plant-plant interactions. Curr. Opin. Plant Biol. 2:65–70.CrossRefGoogle Scholar
Yokota, T., Sakai, H., Okuno, K., Yoneyama, K., and Takeuchi, Y. 1998. Alectrol and orobanchol, germination stimulants for Orobanche minor, from its host red clover. Phytochemistry 49:1967–1973.Google Scholar