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Characterization of a norflurazon-resistant mutant of Chlamydomonas reinhardtii

Published online by Cambridge University Press:  12 June 2017

Varsha Vartak  and
Sujata Bhargava
Varsha Vartak
Affiliation:
Department of Botany, University of Pune, Pune 411 007, India
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Abstract

A norflurazon-resistant mutant has been isolated from Chlamydomonas reinhardtii that showed a three-fold factor of resistance over wild type cultures. In comparison to wild type cultures, the mutant showed better retention of chlorophylls and carotenoids when grown in light in the presence of norflurazon. When grown in the dark, chlorophyll losses were similar, while carotenoid losses were lower than in the wild type cultures. Higher levels of phytoene accumulated in the wild type cultures in the presence of norflurazon than in the resistant cultures. The resistant cultures also showed cross tolerance to EMD-IT 5914, a herbicide with a similar mode of action. Norflurazon resistance in this alga appears to arise from alterations in the target enzyme phytoene desaturase.

Keywords

Norflurazon, 4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3(2H)-pyridazinoneEMD-IT 5914, 5-(dimethyl-aminomethylene)-2-oxo-4-phenyl-2,5-dihydrofurane-carbonitrile-(3)Carotenoidsphytoene desaturaseEMD-IT 5914
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 45 , Issue 3 , June 1997 , pp. 374 - 377
Copyright
Copyright © 1997 by the Weed Science Society of America 

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References

Literature Cited

Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 20: 1–10.Google Scholar
Chamowitz, D., Pecker, I., and Hirschberg, J. 1991. The molecular basis of resistance to herbicide norflurazon. Plant Mol. Biol. 16: 967–974.Google Scholar
Linden, H., Sandmann, G., Chamowitz, D., Hirschberg, J., and Boeger, P. 1990. Generation and biochemical characterisation of Synechococcus mutants selected against the bleaching herbicide norflurazon. Pestic. Biochem. Physiol. 36: 46–51.Google Scholar
Misawa, N., Shigoyuki, Y., de Filipe Hartmut, L., Maria, R., Mercedes, L., Hiroshi, I., and Sandmann, G. 1993. Functional expression of Erwinia uredovora carotenoid biosynthesis gene crtI in transgenic plants showing an increase of beta carotene synthesis activity and resistance to bleaching herbicide norflurazon. Plant J. 4: 833–840.Google Scholar
Sandmann, G. 1993. Spectral determination of carotenoid precursors in Scenedesmus cells treated with bleaching herbicides. in Boeger, P. and Sandmann, G., eds. Target Assays for Modern Herbicides and Related Phytotoxic Compounds. London: Levis Publishers, pp. 3–8.Google Scholar
Sandmann, G. and Boeger, P. 1989. Inhibition of carotenoid biosynthesis by herbicides. in Sandmann, G. and Boeger, P., eds. Target Sites of Herbicide Action. Boca Raton, FL: CRC Press, pp. 25–44.Google Scholar
Sandmann, G. and Kowalczk, S. 1989. In vitro carotenogenesis and characterisation of phytoene desaturase reaction in Anacystis . Biochem. Biophysic. Res. Com. 169: 916–921.Google Scholar
Seber, G.A.F. and Wild, C. J. 1989. Nonlinear Regression. New York: J. Wiley, p. 182.Google Scholar
Snedecor, G. W. and Cochran, W. G. 1994. Statistical Methods. New Delhi: East-West Press, pp. 117–120, 235-236.Google Scholar
Spreitzer, R. J. and Mets, L. 1981. Photosynthesis deficient mutants of Chlamydomonas reinhardtii with associated light sensitive phenotypes. Plant Physiol. 67: 565–569.Google Scholar
Thayer, S. S. and Bjorkman, O. 1990. Leaf xanthophyll content and composition in sun and shade determined by HPLC. Photosynth. Res. 23: 331–343.Google Scholar
Urbach, D., Suchanka, H., and Urbach, W. 1976. Effect of substituted pyridazinone herbicide and difunone (EMD-IT 5914) on carotenoid biosynthesis in green algae. Z. Naturforsch. 31c: 652–655.Google Scholar
Young, A. J. 1991. Inhibition of carotenoid biosynthesis. in Baker, N. R. and Percival, M. P., eds. Herbicides. Amsterdam: Elsevier, pp. 163–168.Google Scholar