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The Basis for Glyphosate Resistance in Rigid Ryegrass (Lolium rigidum) from California

Published online by Cambridge University Press:  20 January 2017

Marulak Simarmata  and
Donald Penner
Marulak Simarmata
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824
Donald Penner*
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824
*
Corresponding author's E-mail: [email protected]
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Abstract

The occurrence of glyphosate-resistant weeds has been reported after more than 20 yr of extensive use. Rigid ryegrass that evolved resistance to glyphosate was found in Australia and in California. Glyphosate-resistant rigid ryegrass plants were collected from northern California and selected through generations 8 and 5 to segregate the most resistant (R) and sensitive (S) biotypes. The eighth generation of R and the fifth generation of S biotypes survived 6.72 and died from 0.11 kg ae ha−1 glyphosate, respectively. The objectives of this study were to evaluate the role of metabolism in the observed resistance, to study the effect of glyphosate on the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), and to characterize the EPSPS gene in R and S rigid ryegrass. Neither quantitative nor qualitative difference was observed in the metabolism of 14C-glyphosate between the biotypes. Activity of constitutive EPSPS decreased more significantly in the S than R biotype in the presence of 5, 50, 500, and 5,000 µM glyphosate. Inhibition of 50% (I50) of the EPSPS activity by glyphosate was more than 90-fold in S compared to R biotype. Decreased EPSPS sensitivity in the R biotype appeared to be a major contributor to glyphosate resistance in rigid ryegrass from California. Fragments of the EPSPS gene containing 1,320 nucleotides were isolated from mRNA of S and R biotypes. A single nucleotide mutation from cytosine (C) to thymine (T) was identified at nucleotide 301 of the truncated EPSPS gene of the R biotype. This mutation changed the amino acid code from proline (Pro) to serine (Ser), which was similar to that reported for the glyphosate-resistant goosegrass from Malaysia and correlated with glyphosate insensitivity of EPSPS.

Keywords

Glyphosategoosegrass, Eleusine indica (L.) Gaertn. ELEINrigid ryegrass, Lolium rigidum Gaud. LOLRIEPSPSglyphosatemetabolismherbicide resistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 2 , April 2008 , pp. 181 - 188
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Baerson, S. R., Rodriguez, D. J., Tran, M., You, J., Kreuger, R. W., Dill, G. M., Pratley, J. E., and Gruys, K. J. 2002a. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 50:721–730.Google Scholar
Baerson, S. R., Rodriguez, D. J., Tran, M., Feng, Y., Biest, N. A., and Dill, G. M. 2002b. Glyphosate resistance goosegrass: identification of mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265–1275.Google Scholar
Boerboom, C. M., Wyse, D. L., and Somers, D. A. 1990. Mechanism of glyphosate tolerance in birdsfoot trefoil (Lotus corniculatus). Weed Sci. 38:463–467.Google Scholar
Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72:248–254.Google Scholar
Chomczynski, P. and Sacchi, N. 1987. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.Google Scholar
Don, R. H., Cox, P. T., Wainwright, B. J., Baker, K., and Mattick, J. S. 1991. Touchdown PCR circumvents spurious priming during gene amplification. Nucleic Acids Res. 19 (14):4008.CrossRefGoogle ScholarPubMed
Feng, P. C. C., Pratley, J. E., and Bohn, J. A. 1999. Resistance to glyphosate in Lolium rigidum. II. Uptake, translocation, and metabolism. Weed Sci. 47:412–415.Google Scholar
Feng, P. C. C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and CaJacob, C. A. 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci. 52:498–505.Google Scholar
Heap, I. and LeBaron, H. 2001. Introduction and overview of resistance. Pages 1–22. in Powles, S. B. and Shaner, D. L. Herbicide Resistance and World Grains. New York CRC.Google Scholar
Lorraine-Colwill, D. F., Hawkes, T. R., Williams, P. H., Warner, S. A. J., Sutton, P. B., Powles, S. B., and Preston, C. 1999. Resistance to glyphosate in Lolium rigidum . Pestic. Sci. 55:486–503.Google Scholar
Lorraine-Colwill, D. F., Powles, S. B., Hawkes, T. R., Hollinshead, P. H., Warner, S. A. J., and Preston, C. 2003. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol. 74:62–72.Google Scholar
Malik, J., Barry, G., and Kishore, G. 1989. The herbicide glyphosate. Biofactors. 2:17–25.Google Scholar
[NCBI] National Center of Biotechnology Information 2007. GenBank: Lolium rigidum EPSPS mRNA, Partial Codons, Locus AF349754. http://www.ncbi.nlm.nih.gov. Accessed: January 20, 2007.Google Scholar
Ng, C. H., Wickneswari, R., Salmijah, S., Teng, Y. T., and Ismail, B. 2003. Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Res. 43:107–115.Google Scholar
Perez-Jones, A., Park, K. W., Colquhoun, J., Mallory-Smith, C., and Shaner, D. 2005. Identification of glyphosate-resistant Italian ryegrass (Lolium multiflorum) in Oregon. Weed Sci. 53:775–779.Google Scholar
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci. 46:604–607.Google Scholar
Pratley, J., Baines, P., Eberbach, R., Incerti, M., and Broster, J. 1996. Glyphosate resistance in annual ryegrass. Abstract 126 in Virgona, J. and Michalk, D. Proceedings of the 11th Annual Conference. Wagga Wagga, Australia Grassland Society of New South Wales.Google Scholar
Sandberg, C. L., Meggitt, W. F., and Penner, D. 1980. Absorption, translocation, and metabolism of 14C-glyphosate in several weed species. Weed Res. 20:195–200.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218–225.Google Scholar
Simarmata, M., Kaufmann, J. E., and Penner, D. 2001. Progress in determining the origin of the glyphosate-resistant ryegrass in California. Abstract 232 in the 41st Meeting. Greensboro, NC Weed Science Society of America.Google Scholar
Simarmata, M., Kaufmann, J. E., and Penner, D. 2003. Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci. 51:678–682.Google Scholar
Sprankle, P., Sandberg, C. L., Meggitt, W. F., and Penner, D. 1978. Separation of glyphosate and possible metabolites by thin-layer chromatography. Weed Sci. 26:673–674.Google Scholar
Tran, M., Baerson, S., and Brinker, R., et al. 1999. Characterization of glyphosate-resistant Eleusine indica biotypes from Malaysia. Pages 527–536. in. Proceedings of the 17th Conference. Bangkok, Thailand Asian-Pacific Weed Science Society.Google Scholar
Wakelin, A. M. and Preston, C. 2006. A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432–440.Google Scholar
Westwood, J. H. and Weller, S. C. 1997. Cellular mechanisms influence differential glyphosate sensitivity in field bindweed (Convolvulus arvensis) biotypes. Weed Sci. 45:2–11.Google Scholar