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Survival of ganglion cells which form the retino-retinal projection during optic nerve regeneration in the frog

Published online by Cambridge University Press:  02 June 2009

M. Tennant ,
S. R. Bruce  and
L. D. Beazley
M. Tennant
Affiliation:
Department of Psychology, University of Western Australia
S. R. Bruce
Affiliation:
Department of Psychology, University of Western Australia
L. D. Beazley
Affiliation:
Department of Psychology, University of Western Australia
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Abstract

During optic nerve regeneration in the frog, axons transiently grow along the opposite optic nerve forming a retino-retinal projection. In the present study, we crushed the left optic nerve in the frog Litoria (Hyla) moorei and later applied horseradish peroxidase (HRP) or diamidino yellow (DY) to the right optic nerve. In one series, retinae were examined 3 days after application of the tracer. The retino-retinal projection was found to be maximal at 5 weeks, fell significantly by 7 weeks, and returned to close-to-normal levels by 24 weeks. In a second series, we applied DY at 5 weeks as before but did not sacrifice the frogs until 7 weeks. Numbers of labeled ganglion cells were not significantly different from those frogs in the first series labeled and examined at 5 weeks. We conclude that ganglion cells giving rise to the retino-retinal projection had not died in appreciable numbers, presumably being sustained by collateral axons in the brain.

Keywords

FrogOptic nerve regenerationRetino-retinal projection
Type
Research Articles
Information
Visual Neuroscience , Volume 10 , Issue 4 , July 1993 , pp. 681 - 686
Copyright
Copyright © Cambridge University Press 1993

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