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Responses of rod bipolar cells isolated from dogfish retinal slices to concentration-jumps of glutamate

Published online by Cambridge University Press:  02 June 2009

R.A. Shiells  and
G. Falk
R.A. Shiells
Affiliation:
Department of Physiology, University College London, Gower St., London WC1E 6BT, UK
G. Falk
Affiliation:
Department of Physiology, University College London, Gower St., London WC1E 6BT, UK
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Abstract

Rod on-bipolar cell light responses are mediated by a class of metabotropic glutamate receptor which is coupled via a G-protein to the control of a cGMP cascade, with cGMP acting to open cation channels, whilst off-bipolar cells possess ionotropic glutamate receptors. Whole-cell voltage-clamp recordings were obtained from on- and off-bipolar cells of dark-adapted dogfish retinal slices, identified by their light responses. Isolated cells were exposed to concentration-jumps of glutamate. At negative voltage-clamp potentials, on-bipolar cells responded to glutamate with outward currents with a mean delay of 10.8 ms, whilst off-bipolar cells responded with inward currents without any delay. Neither cell type showed desensitization to applied steps of glutamate. The dose-response relation for on-bipolar cells showed no gradual saturation, but increased linearly with a sharp cutoff above 200 μM glutamate. This dose-response relation could be fitted with a theoretical expression assuming Michaelis-Menten kinetics for the action of glutamate on receptors and a linear relation between the concentration of receptors bound to glutamate and the fall in cGMP this induces. The dose-response relation of off-bipolar cells showed saturation with a limiting slope of 2 at low glutamate concentrations, suggesting that two molecules of glutamate are required to open each channel by a cooperative mechanism. The glutamate receptor coupled cGMP cascade of rod on-bipolar cells can account for high synaptic voltage gain.

Keywords

Bipolar cellRetinaGlutamate receptorCyclic GMPConcentration-jump
Type
Research Articles
Information
Visual Neuroscience , Volume 11 , Issue 6 , November 1994 , pp. 1175 - 1183
Copyright
Copyright © Cambridge University Press 1994

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