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Recovery from bleaching in photoreceptors promoted by biotin, pyruvate, and glucose

Published online by Cambridge University Press:  02 June 2009

K.N. Leibovic  and
J. Bandarchi
K.N. Leibovic
Affiliation:
State University of New York at Buffalo, Department of Biophysical Sciences, Buffalo
J. Bandarchi
Affiliation:
State University of New York at Buffalo, Department of Biophysical Sciences, Buffalo
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Abstract

In the rods of Bufo marinus and other species, bleaching of the rhodopsin in isolated cells leads to a loss of sensitivity and response amplitude and to a shortened response duration. These changes are permanent for cells bathed in Ringer&s solution. They are due to as yet unknown modulations in the transduction biochemistry. In this paper, we report that these changes can be partly or completely reversed by supplying biotin, pyruvate, and elevated glucose to the rod. The time course of this reversal and the substances which promote it imply that these are metabolically mediated effects. Based on the reported action of biotin and pyruvate on the one hand and on the changes of the response waveforms on the other hand, we believe that the phenomena we observe involve the later steps of the transduction cycle.

Keywords

Vertebrate rodsBleaching adaptationBiotinPyruvateGlucose
Type
Short Communication
Information
Visual Neuroscience , Volume 4 , Issue 5 , May 1990 , pp. 489 - 492
Copyright
Copyright © Cambridge University Press 1990

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