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Inhibition of phospholipase C by U-73122 blocks one component of the receptor current in Limulus photoreceptor

Published online by Cambridge University Press:  02 June 2009

Károly Nagy  and
Klaus Contzen
Klaus Contzen
Affiliation:
Institut fur Biologie II, RWTH Aachen, Kopernikusstr. 16, D-52074 Aachen, Germany
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Abstract

In the ventral nerve photoreceptor of Limulus a short, intense flash evokes a receptor current consisting of three components. In contrast to other hypotheses, we suggested previously that only the second component of the current is activated by the phospholipase C pathway, which releases calcium from intracellular stores by inositol trisphosphate. The present paper gives further evidence to our suggestion. It is demonstrated that U-73122, a specific inhibitor for the phospholipase C, selectively blocks the second component. The first and third components are moderately affected and could still be activated after the complete block of the second one. Results support the idea that the first and third components areactivated by pathways operating independently of phospholipase C.

Keywords

Limulus photoreceptorLight-induced currentMultiple componentsPhospholipase C pathwayCurrent block
Type
Short Communication
Information
Visual Neuroscience , Volume 14 , Issue 5 , September 1997 , pp. 995 - 998
Copyright
Copyright © Cambridge University Press 1997

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