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Immunocytochemical localization of taurine and glial fibrillary acidic protein in human optic nerve

Published online by Cambridge University Press:  02 June 2009

Norma Lake ,
Carole Verdone-Smith  and
Seymour Brownstein
Norma Lake
Affiliation:
Department of Physiology, McGill University Department of Ophthalmology, McGill University
Carole Verdone-Smith
Affiliation:
Department of Physiology, McGill University
Seymour Brownstein
Affiliation:
Department of Ophthalmology, McGill University Department of Pathology, McGill University, Montreal, Quebec, Canada
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Abstract

Taurine immunoreactivity (IR) in 1 -μm sections of glutaraldehyde-fixed human optic nerve was observed using light microscopy and an antibody raised in rabbit to taurine conjugated to bovine serum albumin. Throughout the nerve, taurine-lR was prominent in glial cells, in their perinuclear regions, and in their numerous branching processes, some of which extended to the pial septa. The peripheral glial mantle (glia limitans) was densely stained, whereas axons and the pial septa showed relatively little or no taurine-IR. Immunoreactivity for glial fibrillary acidic protein (GFAP), an astrocyte-specific marker, was evaluated on adjacent sections. The pattern of GFAP-IR was highly similar to that for taurine, suggesting that a subset of taurine-immunoreactive glial cells are optic nerve astrocytes. To our knowledge, this is the first localization of taurine and GFAP in human optic nerve.

Keywords

TaurineGlial fibrillary acidic proteinHuman optic nerveGliaImmunocytochemistry
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 3 , March 1992 , pp. 251 - 255
Copyright
Copyright © Cambridge University Press 1992

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References

Bionami, A., Enc, L.F., Dahl, D. & Uyeda, C.T. (1972). Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Research 43, 429–435.CrossRefGoogle Scholar
Cocker, S.E. & Lake, N. (1987). Electroretinographic alterations and their reversal in rats treated with guanidinoethyl sulfonate, a taurine depletor. Vision Research 8, 389–401.Google Scholar
Gegoel, H.S., Ament, M.E., Heckenlively, J.R., Martin, D.A. & Kopple, J.D. (1985). Nutritional requirement for taurine in patients receiving long-term parenteral nutrition. New England Journal of Medicine 312, 142–146.CrossRefGoogle Scholar
Hocan, M.J., Alvarado, J.A. & Weddell, J.E. (1971). Histology of the Human Eye: An Atlas and Textbook. London: W.B. Saunders Co.Google Scholar
Jacobsen, J.G. & Smith, L.H. (1968). Biochemistry and physiology of taurine and taurine derivatives. Physiological Reviews 48, 424–511.CrossRefGoogle ScholarPubMed
Lake, N. & Malik, N. (1987). Retinal morphology in rats treated with a taurine transport antagonist. Experimental Eye Research 44, 331–346.CrossRefGoogle ScholarPubMed
Lake, N., Malik, N. & De Marte, L. (1988). Taurine depletion leads to loss of optic nerve axons. Vision Research 28, 1071–1076.CrossRefGoogle ScholarPubMed
Lake, N. & Verdone-Smith, C. (1989). Immunocytochemical localization of taurine in the mammalian retina. Current Eye Research 8, 163–173.CrossRefGoogle ScholarPubMed
Lake, N. & Verdone-Smith, C. (1990a). Immunocytochemical localization of taurine within glial cells in the optic nerve of adult albino rats. Current Eye Research 9, 1115–1120.CrossRefGoogle ScholarPubMed
Lake, N. & Verdone-Smith, C. (1990b). Immunocytochemical localization of taurine in the optic nerve of the developing rat. Investigative Ophthalmology and Visual Science (Suppl.) 31, ARVO 159.Google Scholar
Lake, N., Verdone-Smith, C. & Brownstein, S. (1991). Immunocytochemical localization of taurine in glial cells of the rat and human optic nerve. Investigative Ophthalmology and Visual Science (Suppl.) 32, ARVO 1307.Google Scholar
Neuringer, M., Sturman, J.A., Wen, G.Y. & Wisniewski, H.M. (1985). Dietary Taurine Is Necessary For Normal Retinal Development In Monkeys. In Taurine: Biological Actions and Clinical Perspectives, ed. Oja, S.S., Ahtee, L., Kontro, P. & Paasonen, M.K., pp. 53–62. New York: Alan R. Liss.Google Scholar
Pasantes-Morales, H. & Schousboe, A. (1988). Volume regulation in astrocytes: a role for taurine as an osmoeffector. Journal of Neuroscience Research 20, 505–509.CrossRefGoogle ScholarPubMed
Philibert, R.A., Rogers, K.L., Allen, A.J. & Dutton, G.R. (1988). Dose-dependent, K+-stimulated efflux of endogenous taurine from primary astrocyte cultures is Ca2+-dependent. Journal of Neurochemistry 51, 122–126.CrossRefGoogle ScholarPubMed
Schmidt, S.Y., Berson, E.L. & Hayes, K.C. (1976). Retinal degeneration in cats fed casein. 1. Taurine deficiency. Investigative Ophthalmology and Visual Science 15, 47–52.Google ScholarPubMed
Shain, W., Connor, J.N., Madelian, V. & Martin, D.L. (1989). Spontaneous and beta-adrenergic receptor-stimulated release from astroglial cells are independent of manipulations of intracellular calcium. Journal of Neuroscience 9, 2306–2312.CrossRefGoogle Scholar
Vaughan, D.K., Erikson, P.A. & Fisher, S.K. (1990). Glial fibrillary acidic protein (GFAP) immunoreactivity in rabbit retina: effect of fixation. Experimental Eye Research 50, 385–392.CrossRefGoogle ScholarPubMed
Voaden, M.J., Lake, N., Marshall, J. & Morjaria, B. (1977). Studies on the distribution of taurine and other neuroactive amino acids in the retina. Experimental Eye Research 25, 249–257.CrossRefGoogle ScholarPubMed
Voaden, M.J., Oraedu, A.C.I., Marshall, J. & Lake, N. (1981). Taurine in the retina. In The Effects of Taurine on Excitable Tissues, ed. Schaffer, S.W., Baskin, S.I. & Kocsis, J.J., pp. 145–160. New York: Spectrum Publications, Inc.CrossRefGoogle Scholar
Waxman, S.G. & Black, J.A. (1984). Freeze-fracture ultrastructure of the perinodal astrocyte and associated glial junctions. Brain Re-search 308, 77–87.CrossRefGoogle ScholarPubMed