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Immunocytochemical localization of glycine in the retina of the turtle (Pseudemys scripta)

Published online by Cambridge University Press:  02 June 2009

William D. Eldred  and
Kristin Cheung
William D. Eldred
Affiliation:
Department of Biology, Boston University, Boston
Kristin Cheung
Affiliation:
Department of Biology, Boston University, Boston
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Abstract

We have localized glycine-like immunoreactivity to provide new anatomical detail about glycinergic neurons in the turtle retina. A rabbit antiserum directed against a glycine/albumin conjugate was used with standard fluorescent and avidin-biotin labeling techniques. Some processes in the outer plexiform layer and many processes in the inner plexiform layer, numerous somata in the inner nuclear layer, and isolated somata in the ganglion cell layer were immunoreactive.

The vast majority of labeled neurons were amacrine cells. One class of amacrine cells had well-labeled somata near the inner nuclear/inner plexiform layer border, which gave rise to thick primary processes that entered the inner plexiform layer and arborized near the border of strata 1 and 2 and in stratum 3. A second class of glycinergic neurons, consisting of putative interplexiform cells, was unique in that it gave rise to dendritic arborizations in both the outer plexiform layer and the inner plexiform layer. Some of the immunoreactive neurons in the ganglion cell layer were apparently displaced amacrine cells, while others were probably true ganglion cells because they gave rise to labeled axons, and many labeled axons were visible in the ganglion cell axon layer. These results suggested that glycine played an extensive role in the turtle retina, and that it was involved in many diverse synaptic interactions in both the outer plexiform layer and the inner plexiform layer.

Keywords

GlycineImmunocytochemistryRetinaTurtle
Type
Research Article
Information
Visual Neuroscience , Volume 2 , Issue 4 , April 1989 , pp. 331 - 338
Copyright
Copyright © Cambridge University Press 1989

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References

Ball, A.K. & Brandon, C. (1986). Localization of [3H]-GABA, -muscimol, and -glycine in goldfish retinas stained for glutamate decarboxylase. Journal of Neuroscience 6, 1621–1627.CrossRefGoogle ScholarPubMed
Cajal, S.R.Y. (1933). Die Retina der Wirbeltiere. Weisbaden: Bergmann. (Translated by Thorpe, S.A. & Glickstein, M. (1972). The Structure of the Retina. Springfield: Thomas.)Google Scholar
Cohen, E. & Sterling, P. (1986). Accumulation of [3H]-glycine by cone bipolar neurons in the cat retina. Journal of Comparative Neurology 250, 1–7.CrossRefGoogle ScholarPubMed
Eldred, W.D. & Karten, H.J. (1983). Characterization and quantification of peptidergic amacrine cells in the turtle retina: enkephalin, neurotensin, and glucagon. Journal of Comparative Neurology 221, 371–381.CrossRefGoogle ScholarPubMed
Eldred, W.D. & Carraway, R.E. (1987). Neurocircuitry of two types of neurotensin-containing amacrine cells in the turtle retina. Neuroscience 21, 603–618.CrossRefGoogle ScholarPubMed
Jager, J. & Wassle, H. (1987). Localization of glycine uptake and receptors in the cat retina. Neuroscience Letters 75, 147–151.CrossRefGoogle ScholarPubMed
Kleinschmidt, J. & Yazulla, S. (1984). Uptake of [3H]-glycine in the outer plexiform layer of the retina of the toad (Bufo marinus). Journal of Comparative Neurology 230, 352–360.CrossRefGoogle ScholarPubMed
Kolb, H. (1982). The morphology of the bipolar cells, amacrine cells, and ganglion cells in the retina of the turtle (Pseudemys scripta elegans). Philosophical Transactions of the Royal Society B (London) 298, 355–393.Google ScholarPubMed
Kolb, H. (1988). Neural organization of the retina of the turtle (Mauremys caspica): a light microscopic and Golgi study. Visual Neuroscience 1, 47–72.CrossRefGoogle ScholarPubMed
Kolb, H. & Nelson, R. (1984). Neural architecture of the cat retina. In Progress in Retinal Research, Vol. 3, ed. Osborne, N. & Chader, J., pp. 21–60. New York: Pergamon Press.Google Scholar
Kolb, H., Nelson, R. & Mariani, A. (1981). Amacrine cells, bipolar cells, and ganglion cells of the cat retina: a Golgi study. Vision Research 21, 1081–1114.CrossRefGoogle ScholarPubMed
Marc, R.E. (1982). Spatial organization of neurochemically classified interneurons of the goldfish retina. I. Local patterns. Vision Research 22, 589–608.CrossRefGoogle ScholarPubMed
Marc, R.E. (1985). The role of glycine in retinal circuitry. In Retinal Transmitters and Modulators: Models for the Brain, ed. MORGAN, W.W., pp. 119–158. Boca Raton: CRC Press.Google Scholar
Marc, R.E. & Lam, D.M.-K. (1981). Glycinergic pathways in goldfish retina. Journal of Neuroscience 1, 152–165.CrossRefGoogle ScholarPubMed
Marc, R.E. & Liu, W.-L.S. (1985). [3H]-glycine-accumulating neurons of the human retina. Journal of Comparative Neurology 232, 241–260.CrossRefGoogle Scholar
Massey, S.C. & Redburn, D.A. (1987). Transmitter circuits in the vertebrate retina. Progress in Neurobiology 28, 55–96.CrossRefGoogle ScholarPubMed
Pourcho, R.G. (1980). Uptake of [3H]-glycine and [3H]-GABA by amacrine cells in the cat retina. Brain Research 198, 333–346.CrossRefGoogle ScholarPubMed
Pourcho, R.G. & Goebel, D.J. (1985). Immunocytochemical demonstration of glycine in retina. Brain Research 348, 187–191.CrossRefGoogle ScholarPubMed
Pourcho, R.G. & Goebel, D.J. (1987). Visualization of endogenous glycine in cat retina: an immunocytochemical study with Fab fragments. Journal of Neuroscience 7, 1189–1197.CrossRefGoogle ScholarPubMed
Priest, T.D., Robbins, J. & Ikeda, H. (1985). The action of inhibitory neurotransmitters, γ-aminobutyric acid and glycine may distinguish between the area centralis and the peripheral retina in cats. Vision Research 25, 1761–1770.CrossRefGoogle ScholarPubMed
Rayborn, M.E., Sarthy, P.V., Lam, D.M.-K. & Hollyfield, J.G. (1981). The emergence, localization, and maturation of neurotrans-mitter systems during the development of the retina in Xenopus laevis. II. Glycine. Journal of Comparative Neurology 195, 585–593.CrossRefGoogle Scholar
Storm-Mathisen, J., Leknes, A.K., Bore, A.T., Vaaland, J.L., Edminson, P., Haug, F.M.S. & Otterson, O.P. (1983). First visualization of glutamate and GABA in neurons by immunocyto-chemistry. Nature 301, 517–520.CrossRefGoogle Scholar
Watt, C.B., Li, H.B. & Lam, D.M.-K. (1985). The presence of three neuroactive peptides in putative glycinergic amacrine cells in avian retina. Brain Research 348, 187–191.CrossRefGoogle ScholarPubMed
Weiler, R. & Ball, A.K. (1984). Co-localization of neurotensin-like immunoreactivity and [3H]-glycine uptake in sustained amacrine cells of turtle retina. Nature 311, 759–761.CrossRefGoogle ScholarPubMed
Weiler, R. & Schutte, M. (1985). Morphological and pharmacological analysis of putative serotonergic bipolar and amacrine cells in the retina of a turtle (Pseudemys scripta elegans). Cell and Tissue Research 241, 373–382.CrossRefGoogle ScholarPubMed
Wenthold, R.J., Hum, D., Altschuler, R.A. & Reeks, K.A. (1987). Glycine immunoreactivity localized in the cochlear nucleus and superior olivary complex. Neuroscience 11, 897–912.CrossRefGoogle Scholar
Yang, C.-Y. & Yazulla, S. (1988). Light-microscopic localization of putative glycinergic neurons in the larval tiger salamander retina by immunocytochemical and autoradiographical methods. Journal of Comparative Neurology 272, 343–357.CrossRefGoogle ScholarPubMed