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Identification of a G-protein in depolarizing rod bipolar cells

Published online by Cambridge University Press:  02 June 2009

Noga Vardi ,
Diane F. Matesic ,
David R. Manning ,
Paul A. Liebman  and
Peter Sterling
Noga Vardi
Affiliation:
Department of Anatomy, University of Pennsylvania, Philadelphia
Diane F. Matesic
Affiliation:
Department of Pediatrics/Human Development, B240 Life Sciences, Michigan State University, E. Lansing
David R. Manning
Affiliation:
Department of Pharmacology, University of Pennsylvania, Philadelphia
Paul A. Liebman
Affiliation:
Department of Anatomy, University of Pennsylvania, Philadelphia
Peter Sterling
Affiliation:
Department of Anatomy, University of Pennsylvania, Philadelphia
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Abstract

Synaptic transmission from photoreceptors to depolarizing bipolar cells is mediated by the APB glutamate receptor. This receptor apparently is coupled to a G-protein which activates cGMP-phosphodiesterase to modulate cGMP levels and thus a cGMP-gated cation channel. We attempted to localize this system immunocytochemically using antibodies to various components of the rod phototransduction cascade, including Gt (transducin), phosphodiesterase, the cGMP-gated channel, and arrestin. All of these antibodies reacted strongly with rods, but none reacted with bipolar cells. Antibodies to a different G-protein, Go, reacted strongly with rod bipolar cells of three mammalian species (which are depolarizing and APB-sensitive). Also stained were subpopulations of cone bipolar cells but not the major depolarizing type in cat (b1). Go antibody also stained certain salamander bipolar cells. Thus, across a wide range of species, Go is present in retinal bipolar cells, and at least some of these are depolarizing and APB-sensitive.

Keywords

PhosphodiesterasecGMP-gated channelTransducinArrestinG-proteinRetinaRod bipolar
Type
Articles
Information
Visual Neuroscience , Volume 10 , Issue 3 , May 1993 , pp. 473 - 478
Copyright
Copyright © Cambridge University Press 1993

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